Fermentation technology

Stanbury, Peter F.

doi:10.1039/9781847551498-00001

Cited by 42 publications

(49 citation statements)

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“…The most effectual fermentation variables, which influence the protease production maximally can be identified by using the traditional 'one-variableat-a-time' approach or by PB designed experiments (Bajaj and Wani 2011;Mukherjee et al 2012). Since the PB design study is fast, less laborious, efficient and reliable, is widely preferred choice (Stanbury et al 1995). Among a total of six fermentation variables examined, four (incubation time, soybean meal, mustard cake and molasses) influenced the protease production maximally by B. subtilis I-2.…”

Section: Discussionmentioning

confidence: 99%

Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues

Bajaj¹,

Singh²,

Khullar³

et al. 2014

Braz. arch. biol. technol.

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Section: Discussionmentioning

confidence: 99%

Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues

Bajaj¹,

Singh²,

Khullar³

et al. 2014

Braz. arch. biol. technol.

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“…Similar findings were reported earlier by Nair et al (2013) and Kalyanasundaram et al (2015). At longer incubation periods, the enzyme activity decreased which might be due to depletion of nutrients, accumulation of toxic end products and change in the pH of the medium or loss of moisture content (Stanbury et al, 1997).…”

Section: Effect Of Different Incubation Periodsmentioning

confidence: 99%

Production of Extracellular Anti-leukemic Enzyme L-asparaginase from Fusarium solani AUMC 8615 Grown under Solid-State Fermentation conditions: Purification and Characterization of The Free and Immobilized Enzyme

2016

Egypt. J. Bot.

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OTENTIALITIES of twenty four fungal isolates were investigated for their ability to produce L-asparaginase using modified Czapek's Dox medium. Fusarium solani AUMC 8615 was selected as the most potent fungal strain for the enzyme production under solid state fermentation (SSF) using agro-industrial residues. Wheat bran supported maximum enzyme production followed by rice bran and corn cob. The optimum conditions for maximum production of L-asparaginase under SSF occurred on the fifth day of incubation at 30°C, pH 8.0, 60% of initial moisture content and supplementation with 0.2% (w/v) glucose and 0.5% (w/v) ammonium sulphate. Lasparaginase was purified to homogeneity by ammonium sulphate precipitation, gel filtration and ion exchange chromatography giving 299.58 purification fold. SDS-PAGE showed that the purified enzyme consists of two subunits of molecular weights 70 and 80 kDa, respectively. The enzyme was efficiently immobilized by covalent binding with activated charcoal giving an immobilization yield of 80.40%. Optimum pH values were 9.0 and 8.0 for free and immobilized enzyme, respectively. While, Optimum reaction temperatures were 37°C and 45°C for free and immobilized enzyme, respectively. After incubation for 2 hr at 37°C, the relative activity of free enzyme decreases to 35% whereas for the immobilized enzyme decreased only to 57% at 45°C.

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“…There are several ways that cultures of H. bacteriophora can be grown: shake flasks, stirred bioreactors [54], airlift bioreactors [55] and in vivo production. Nematode inoculum densities can vary between 300-4,000 nematodes per milliliter.…”

Section: Mass Production In Liquid Mediamentioning

confidence: 99%

Mass Production of the Beneficial Nematode Heterorhabditis bacteriophora and Its Bacterial Symbiont Photorhabdus luminescens

2012

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Entomoparasitic nematodes (EPNs) are being commercialized as a biocontrol measure for crop insect pests, as they provide advantages over common chemical insecticides. Mass production of these nematodes in liquid media has become a major challenge for commercialization. Producers are not willing to share the trade secrets of mass production and by doing so, have made culturing EPNs extremely difficult to advance existing technologies. Theoretically, mass production in liquid media is an ideal culturing method as it increases cost efficiency and nematode quantity. This paper will review current culturing methodologies and suggest basic culturing parameters for mass production. This review is focused on Heterorhabditis bacteriophora; however, this information can be useful for other nematode species.

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Fermentation technology

Cited by 42 publications

References 0 publications

Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues

Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues

Production of Extracellular Anti-leukemic Enzyme L-asparaginase from Fusarium solani AUMC 8615 Grown under Solid-State Fermentation conditions: Purification and Characterization of The Free and Immobilized Enzyme

Mass Production of the Beneficial Nematode Heterorhabditis bacteriophora and Its Bacterial Symbiont Photorhabdus luminescens

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