Fermentation strategies to improve propionic acid production with<i>propionibacterium</i>ssp.: a review

Collograi, Karen Cristina; Costa, Aline Carvalho da; Ienczak, Jaciane Lutz

doi:10.1080/07388551.2021.1995695

Cited by 6 publications

(1 citation statement)

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“…To date, high-level PA production has been achieved through the Wood-Werkman cycle of several candidates belonging to the genus Propionibacteria and the reductive acrylate pathway of Clostridium propionicum under anaerobic conditions using glucose or glycerol as the carbon source (Gonzalez-Garcia et al, 2017a;Collograi et al, 2022). However, Propionibacteria or Clostridium-based PA production has several limitations, including slow growth rates and costly downstream processing due to the high amount of by-products generated during fermentation (Ranaei et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Bioproduction of propionic acid using levulinic acid by engineered Pseudomonas putida

Tiwari

Sathesh‐Prabu²,

Lee³

2022

Front. Bioeng. Biotechnol.

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The present study elaborates on the propionic acid (PA) production by the well-known microbial cell factory Pseudomonas putida EM42 and its capacity to utilize biomass-derived levulinic acid (LA). Primarily, the P. putida EM42 strain was engineered to produce PA by deleting the methylcitrate synthase (PrpC) and propionyl-CoA synthase (PrpE) genes. Subsequently, a LA-inducible expression system was employed to express yciA (encoding thioesterase) from Haemophilus influenzae and ygfH (encoding propionyl-CoA: succinate CoA transferase) from Escherichia coli to improve the PA production by up to 10-fold under flask scale cultivation. The engineered P. putida EM42:ΔCE:yciA:ygfH was used to optimize the bioprocess to further improve the PA production titer. Moreover, the fed-batch fermentation performed under optimized conditions in a 5 L bioreactor resulted in the titer, productivity, and molar yield for PA production of 26.8 g/L, 0.3 g/L/h, and 83%, respectively. This study, thus, successfully explored the LA catabolic pathway of P. putida as an alternative route for the sustainable and industrial production of PA from LA.

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Section: Introductionmentioning

confidence: 99%