Fermentation of Waste Fruits for Bioethanol Production

Jadhav

Int. J. Environ. Sci. Technol.

2015

The potential environmental benefits that can be obtained from replacing petroleum fuels with biofuels derived from renewable biomass sources are the main driving forces for promoting the production and use of biofuels. Due to depletion of fossil fuels, ethanol, which can be obtained via the bioconversion of renewable feedstock, is widely regarded as an efficient alternative for gasoline as transportation fuel. Biomass energy can play an important role in reducing greenhouse gas emissions. Rice bran is a by-product of milling process of rice, and due to its carbohydrate contents, it may serve as good source for bioethanol production. The present study deals with bioethanol production from rice bran and screening of bioethanol-producing bacteria from rice bran. In the screening process, three fermentative bacteria were obtained; they were studied on the basis of morphology, biochemical characteristics and maximum bioethanol production. The maximum bioethanol-producing bacteria was identified by sequencing method. The bacteria thus identified as Bacillus cereus strain McR-3 is a novel bacteria reported in bioethanol production from rice bran substrate. Different parameters like temperature and pH also affects the production of bioethanol. It was observed that optimum temperature and pH for maximum bioethanol production was 37°C and 5, respectively.

Section: Introductionmentioning

confidence: 99%

Bioethanol production from rice bran with optimization of parameters by Bacillus cereus strain McR-3

Jadhav

Int. J. Environ. Sci. Technol.

2015

J microb biotech food sci

“…Candida albicans, Kluyveromyces lactis, K. marxianus, Saccharomyces cerevisiae, Pichia stipites, Lipomyces starkeyi, Hansenula polymorpha, and C. utilis were the few yeast species containing ADH enzyme (Karaoğlan et al, 2020;Suwannarangsee et al, 2010;Mazzoni et al, 1992;Verduyn et al, 1988;Heick and Barrette, 1970). Organic wastes are sugar-rich and low-cost substrates for ethanol production, as well as a desirable environment for ethanol-tolerant fermentable microorganisms (Arshad et al, 2019;Tiwari et al, 2013). External factors and microbes' development usually damage fruits, and its low pH and high sugar content encourage yeast, molds, and acid-tolerant bacteria growth.…”

Section: Introductionmentioning

confidence: 99%

Screening and Characterization of Potential Ethanol Oxidizing Yeast Isolates From Sugar-Rich Organic Waste Resources Using Schiff’s-Base Reaction

Subashri

Mahalingam²

2023

Alcohol dehydrogenase is an oxidoreductase enzyme that carries both alcohol oxidation and aldehyde/ketone reduction activity. Alcohol dehydrogenase (ADH) activity of yeast cells were essential for industrial applications. Sugar-rich organic waste resources including spoiled fruits, fruit peel waste, spoiled vegetables, raw and composed sludge from the dairy industry, and cane molasses spill from the sugar industry, provide a favorable habitat for ethanol-oxidizing microorganisms with ADH activity. The total ethanol oxidizing microbial population of organic waste resources differs considerably from the overall microbial community. Yeast from the natural habitats has a great potential for developing novel enzymes for industrial uses. Under this backdrop, the present study was focused on screening and characterization of potential ethanol oxidizing yeast strains from sugar-rich organic waste resources. Totally seventeen ethanol oxidizing yeast strains belongs to Pichia sp. (9 strains), Candida sp. (2 strains) and Debaryomyces sp. (2 strains), Hanseniaspora sp, Lachancea sp, Torulaspora sp, and Magnusiomyces sp. have been identified. The ability of strains to oxidize ethanol into acetaldehyde was verified by the Schiff’s-base reaction through p-rosaniline assay. The selected five potential ethanol oxidizing strains were identified by 18S rRNA gene sequence as Pichia kluyveri FYES5, Pichia kudriavzevii FYES6, Pichia kudriavzevii VYES2, Candida mengyuniae CSYES1, and Magnusiomyces capitatus CSYES2 with the p-r index of 2.64±0.40, 3.79±0.54, 3.73±0.69, 2.28±0.24, and 2.87±0.58 respectively at 28°C. This is the first report to identify ethanol oxidizing yeast strains from sugar-rich organic waste resources and by this study found that the strain Candida mengyuniae has a better ability to oxidize ethanol.

“…The selection of feedstock is an important part of bioethanol production. Some researchers used cereals like barley, maize, oat and sugar beet [4][5][6][7][8][9]29 , jatropha oil cake, Azolla, waste fruits, deoiled rice bran and rice bran for bioethanol production.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Sachharification and Fermentation of Rice Residues and its Comparative Analysis for Bioethanol Production

Sinha¹,

Tiwari²,

Jadhav³

2019

Def. Life. Sc. Jl.

Energy consumption has inflated steadily over the last century because the world population has fully grown and additional countries became industrialised. Bioethanol is an alcohol produced by fermentation of plant biomass, containing carbohydrate and its production depends upon feedstock availability, variability, and sustainability. The selection of feedstock and its pretreatment is an important part of bioethanol production process. In present work, the exploration of the potential of agro-waste rice residues such as, rice bran and rice husk was done, because it contains sufficient amount of carbohydrate which can be ferment into bioethanol. The aim of the research was also to investigate how different pretreatment methods with moderate conditions differ in hydrolysis and fermentation efficiencies. Pretreatment plays an important role in the hydrolysis of cellulose and lignocellulose. It was found that biological pretreatment was a most effective method in terms of production of bioethanol and it enhances the production as well as fermentation efficiency.