Fermentation of simple and complex substrates to biohydrogen using pure Bacillus cereus RTUA and RTUB strains

Saleem, Aimen; Umar, H Umar H; Shah, Tawaf Ali; Tabassum, Romana

doi:10.1016/j.eti.2020.100704

Cited by 25 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proteases have thermostable activity at optimal temperature ranges of up to 60°C, making them a perfect t for the detergent industry. Such thermostable proteases can also be utilized to reduce the chance of contamination by using procedures that are inappropriate for mesophilic microbes [24]. Proteases are useful in the detergent industries as they are stable in surfactants [21].…”

Section: Discussionmentioning

confidence: 99%

Production of Alkaline protease from Bacillus amyloliquefaciens TAS-2 and Optimizing fermentation conditions

SHAH,

Li,

Zhang

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

The aim of the present investigation was to standardize the conditions of batch fermentation for the production of a commercially significant alkaline protease from Bacillus amyloliquefaciens TAS-2. The B. amyloliquefaciens TAS-2 exhibited a distinct zone of clearance on the skim milk agar medium and demonstrated an enzyme activity of 38.6 U/mL after 24 hours of incubation at a pH of 7 and a temperature of 37°C. B. amyloliquefaciens TAS-2 was identified through genotypic analysis using 16S rRNA sequencing. The highest protease activity was obtained after 24 hours of incubation time, at pH 9, and temperature of 40°C. Similarly, maximum activity was observed with an agitation speed of 150 rpm, an inoculum age of 24 hours, an inoculum volume of 3% v/v, a substrate concentration of 3%, and a flask capacity of 250 mL. The activity was positively enhanced with addition of various nitrogen and carbon sources. Similarly, the presence of amino acids and metal ions induced protease production. However, the addition of Fe2 + and Zn2 + ions at specific concentrations in the medium was found to be inhibitory. Conversely, the addition of Mg2 + and Ca2 + ions had a stimulating effect on protease production. All the optimized parameters were incorporated into the basal medium, and fermentation was conducted under optimal conditions. The precipitation of the maximum amount of protein was achieved at 70–80% saturation of ammonium sulfate. The protease activity was 1.56 time higher for the partially purified protease compared to the crude supernatant. The partially purified protease exhibited optimum activity at a temperature of 55°C and a pH of 9. At 5 mM, PMSF significantly suppressed enzyme activity, whereas Triton X-100 and CTAB increased enzyme activity. Among the different metal ions tested, Ca2+ (5 mM), Mg2+ (5 mM), and Mn2+ (5 mM) stimulated enzyme activity, while Zn2 + and Fe2 + decreased protease activity. The enzyme demonstrated remarkable stability, retaining its activity even after being heated to 60°C for 60 minutes and remaining stable within a pH range of 8 to 11. The study suggests that the alkaline protease of B. amyloliquefaciens TAS-2 that is thermotolerant and surfactant stable can have potential applications across different industries due to its ability to improve yield and properties.

show abstract

Section: Discussionmentioning

confidence: 99%

Production of Alkaline protease from Bacillus amyloliquefaciens TAS-2 and Optimizing fermentation conditions

SHAH,

Li,

Zhang

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Various renewable organic wastes such as sake lees, cassava, sago, glycerol, rice straw, vegetable waste, food waste, date seeds, sugarcane molasses, corn stover, alligator weed, oil palm sap and wheat straw have been explored as the potential substrate for dark fermentative biohydrogen production (Chen et al 2021 ; Choiron et al 2020 ; Li et al 2020 ; Liu et al 2013 ; Moreno-Andrade et al 2015 ; Noparat et al 2012 ; Oliveira et al 2020 ; Panin et al 2020 ; Pason et al 2020 ; Rambabu et al 2020 ; Saleem et al 2020 ; Ulhiza et al 2018 ; Zhang et al 2011 ). Palm oil mill effluent (POME), a wastewater generated in large quantity during palm oil extraction process is another renewable organic waste of interest that is currently under intense investigations as biohydrogen production substrate (Abdullah et al 2020 ; Akhbari et al 2021 ; Audu et al 2021 ; Jamali et al 2019 ; Zainal et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Microbiomes of biohydrogen production from dark fermentation of industrial wastes: current trends, advanced tools and future outlook

Dzulkarnain

Audu

Dagang

et al. 2022

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Biohydrogen production through dark fermentation is very attractive as a solution to help mitigate the effects of climate change, via cleaner bioenergy production. Dark fermentation is a process where organic substrates are converted into bioenergy, driven by a complex community of microorganisms of different functional guilds. Understanding of the microbiomes underpinning the fermentation of organic matter and conversion to hydrogen, and the interactions among various distinct trophic groups during the process, is critical in order to assist in the process optimisations. Research in biohydrogen production via dark fermentation is currently advancing rapidly, and various microbiology and molecular biology tools have been used to investigate the microbiomes. We reviewed here the different systems used and the production capacity, together with the diversity of the microbiomes used in the dark fermentation of industrial wastes, with a special emphasis on palm oil mill effluent (POME). The current challenges associated with biohydrogen production were also included. Then, we summarised and discussed the different molecular biology tools employed to investigate the intricacy of the microbial ecology associated with biohydrogen production. Finally, we included a section on the future outlook of how microbiome-based technologies and knowledge can be used effectively in biohydrogen production systems, in order to maximise the production output.

show abstract

New Development in Renewable Energy Research

Shah

Li²,

Li³

et al. 2023

Environmental Science and Engineering

View full text Add to dashboard Cite

Fermentation of simple and complex substrates to biohydrogen using pure Bacillus cereus RTUA and RTUB strains

Cited by 25 publications

References 47 publications

Production of Alkaline protease from Bacillus amyloliquefaciens TAS-2 and Optimizing fermentation conditions

Production of Alkaline protease from Bacillus amyloliquefaciens TAS-2 and Optimizing fermentation conditions

Microbiomes of biohydrogen production from dark fermentation of industrial wastes: current trends, advanced tools and future outlook

New Development in Renewable Energy Research

Contact Info

Product

Resources

About