Fermentation of raw glycerol to 1,3-propanediol by new strains ofClostridium butyricum

Several microorganisms are known for their efficient anaerobic conversion of glycerol to 1,3-propanediol, with Clostridium diolis DSM 15410 as one of the better performers in terms of molar yield and volumetric productivity. However, this performance is still insufficient to compete with established chemical processes. Previous studies have shown that high concentrations of 1,3-propanediol, glycerol, and fermentation side products can limit the productivity of C. diolis DSM 15410. Here, we describe the use of genome shuffling for improved 1,3-propanediol fermentation by the strict anaerobe C. diolis DSM 15410. By using chemical mutagenesis, strains with superior substrate and product tolerance levels were isolated and higher product yields were obtained. These superior strains were then used for genome shuffling and selection for 1,3-propanediol and organic acid tolerance. After four rounds of genome shuffling and selection, significant improvements were observed, with one strain attaining a 1,3-propanediol volumetric yield of 85 g/liter. This result represents an 80% improvement compared to the yield from the parental wild-type strain.

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“…Previous investigations showed that crude glycerol can be used as the sole carbon source for 1,3-PD production by C. butyricum (18,27).…”

Section: Discussionmentioning

confidence: 99%

Genome Shuffling in Clostridium diolis DSM 15410 for Improved 1,3-Propanediol Production

Otte

Grunwaldt

Mahmoud

et al. 2009

Appl Environ Microbiol

109

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“…However, the use of the engineered strains has rarely been tested with substrates of technical quality. For raw glycerol qualities, it has been observed that production of 1,3-propanediol, butanol, and lipids from glycerol by Clostridium species or by Yarrowia lipolytica (6,20,41,43,46) is influenced by the glycerol purity, and the resistance of C. glutamicum to impurities present in raw glycerol remains to be studied. The C. glutamicum strains for amino acid production from glycerol described here might prove valuable for efficient use of glycerol, which arises in large quantities in the biodiesel process as a major by-product of plant seed oil transesterification with methanol.…”

Section: Discussionmentioning

confidence: 99%

Engineering of a Glycerol Utilization Pathway for Amino Acid Production by Corynebacterium glutamicum

Rittmann

Lindner²,

Wendisch³

2008

Appl Environ Microbiol

130

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The amino acid-producing organism Corynebacterium glutamicum cannot utilize glycerol, a stoichiometric by-product of biodiesel production. By heterologous expression of Escherichia coli glycerol utilization genes, C. glutamicum was engineered to grow on glycerol. While expression of the E. coli genes for glycerol kinase (glpK) and glycerol 3-phosphate dehydrogenase (glpD) was sufficient for growth on glycerol as the sole carbon and energy source, additional expression of the aquaglyceroporin gene glpF from E. coli increased growth rate and biomass formation. Glutamate production from glycerol was enabled by plasmid-borne expression of E. coli glpF, glpK, and glpD in C. glutamicum wild type. In addition, a lysine-producing C. glutamicum strain expressing E. coli glpF, glpK, and glpD was able to produce lysine from glycerol as the sole carbon substrate as well as from glycerol-glucose mixtures.

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“…Alternatively, several species of clostridia, including C. butylicum [52], Clostridium pasteurianum [53][54][55], and Clostridium butyricum [52,[55][56][57][58][59], are also capable of utilizing glycerol as the sole carbon source under anaerobic conditions. In general, these bacteria are the best natural option for glycerol fermentation, in that they are not pathogenic, and in some species the pathway for glycerol utilization is not dependent on the presence of vitamin B 12 [60,61].…”

Section: Biological Conversion Of Glycerolmentioning

confidence: 99%

The glycerin glut: Options for the value‐added conversion of crude glycerol resulting from biodiesel production

Johnson¹,

Taconi²

2007

Environ. Prog.

758

442

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Dramatic increases in the price of crude oil, and consequently, transportation fuels, coupled with increased environmental concerns have resulted in rapid growth in biodiesel production, both in the United States and worldwide. As biodiesel production increases, so does production of the primary coproduct, glycerol. Since the existing glycerol supply and demand market was tight, recent increases in glycerol production from biodiesel refining has created a glut in the glycerol market. As a result, the price of glycerol has fallen significantly and biodiesel refiners are faced with limited options for managing the glycerol by‐product, which in the biodiesel industry, has essentially become a waste stream. This article is a review of promising options for both the catalytic and biological conversion of glycerol into various value‐added products, many of which are bio‐based alternatives to petroleum‐derived chemicals. © 2007 American Institute of Chemical Engineers Environ Prog, 26: 338–348, 2007

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Fermentation of raw glycerol to 1,3-propanediol by new strains ofClostridium butyricum

Cited by 108 publications

References 12 publications

Genome Shuffling in Clostridium diolis DSM 15410 for Improved 1,3-Propanediol Production

Genome Shuffling in Clostridium diolis DSM 15410 for Improved 1,3-Propanediol Production

Engineering of a Glycerol Utilization Pathway for Amino Acid Production by Corynebacterium glutamicum

The glycerin glut: Options for the value‐added conversion of crude glycerol resulting from biodiesel production

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