Fermentation of glucose and xylose in <i>Prevotella ruminicola</i> AR29

Abstract

Prevotella ruminicola is a fibrolytic rumen bacterium that degrades complex carbohydrates and ferments the resulting hexoses and pentoses. Cultures of strain AR29 grew more rapidly on glucose than on xylose, but produced almost the same metabolite profiles on both carbon sources. Glucose in glucose-plus-xylose medium was used preferentially. The production of cell dry matter, growth yields of dry matter and protein, and cell composition (cell carbohydrate and protein) were not significantly different in glucose-and xylose-grown cultures. High aldolase (EC 4.1.2.13) activity was found both in glucose-and xylose-grown cells of the strain AR29. A very low phosphoketolase (EC 4.1.2.9) activity was detected in cells cultured on xylose. In contrast, enzymes of the EntnerDoudoroff pathway (6-phosphogluconate dehydrase and 2-keto-3-deoxy-6-phosphogluconate aldolase) could not be evidenced. KEY WORDS: Prevotella ruminicola, metabolism, carbohydrates INTRODUCTIONAnaerobic bacteria belonging to the species Prevotella ruminicola represent one of the most numerous bacterial groups inhabiting the rumen (Stewart and Bryant, 1988). The predominance of this organism can be explained by its ability to use a variety of substrates and also by its efficient energy metabolism. Strains that have been isolated from the rumen ferment soluble sugars including soluble cellodextrins, polysaccharides, and decompose protein, peptides and amino acids. Several studies attempted to elucidate carbohydrate metabolism of P. ruminicola. Experiments by Joyner and Baldwin (1966) and Mountfort and Roberton (1978)

Metabolism of pectin in rumen bacteria Butyrivibrio fibrisolvens and Prevotella ruminicola

“…The taxonomy of this isolate with respect to suggestions of Avguštin et al . (1997) has been discussed recently by Kovár et al . (1999) .…”

Improving the Efficiency of Rumen Function: When to Intervene

“…The abundance of P. ruminicola is associated with the predicted high gene frequency for propionate metabolism at day 137. P. ruminicola produces propionate along with other VFAs from glucose and xylose fermentation (Kovár et al 1999). Strobel (1993) reported the utilisation of pentose in plant cell walls by P. ruminicola, which could be reflected in high numbers of pentose and glucuronate interconversion genes, observed in this study.…”

“…In the current work, the most abundant OTU classified as Prevotella (OTU 246608), had 92% sequence similarity to Prevotella ruminicola (Purushe et al 2010). Prevotella ruminicola is a fibrolytic rumen bacterium that degrades complex carbohydrates (Kovár et al 1999) with propionate as a primary end product of fermentation (Strobel 1992). Succinisclasticum (OTU 204600) had 95% sequence similarity to Succinisclasticum ruminis (Van Gylswyk 1995).…”

“…The results of the study showed that the profile of the liquid-associated bacteria community was clustered according to the diet treatments (Abecia et al 2014b). This was expected as the liquidassociated bacteria are dominated by Prevotellaceae which is well known as generalist bacteria which utilise various substrates including proteins and starches (Wallace et al 1997a;Kovár et al 1999;Walker et al 2005).…”

Effects of yeast culture and oxalic acid supplementation on in vitro nutrient disappearance, rumen fermentation, and bacterial community composition

“…Prevotella strains have been found to ferment hemicellulose but exhibit differences in fermentation end products (58). It contains the Prevotella ruminicola species which plays a role in nitrogen metabolism and is also a fibrolytic bacteria that possesses carbohydrate esterases, facilitating hemicellulose degradation (59). The Butyrivibrio genus can also degrade hemicellulose (arabinoxylans) and pectin and produce butyrate (60).…”