Fermentation of 12% (w/v) Glucose to 1.2 m Lactate by Escherichia coli Strain SZ194 using Mineral Salts Medium

Zhou, Shengde; Shanmugam, K. T.; Yomano, Lorraine P.; Grabar, Tammy Bohannon; Ingram, L. O.

doi:10.1007/s10529-006-0032-5

Cited by 75 publications

(43 citation statements)

References 40 publications

(41 reference statements)

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“…Such a strain is unable to recycle NADH, thereby creating a driving force for reactions that consume NADH. Similar strategies have been successfully applied to the production of (D/L)-lactate (49), succinate (26), ethanol (29,44), and L-alanine (46) in E. coli.…”

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confidence: 98%

Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli

Shen

Lan

Dekishima

et al. 2011

Appl Environ Microbiol

565

508

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1-Butanol, an important chemical feedstock and advanced biofuel, is produced by Clostridium species. Various efforts have been made to transfer the clostridial 1-butanol pathway into other microorganisms. However, in contrast to similar compounds, only limited titers of 1-butanol were attained. In this work, we constructed a modified clostridial 1-butanol pathway in Escherichia coli to provide an irreversible reaction catalyzed by trans-enoyl-coenzyme A (CoA) reductase (Ter) and created NADH and acetyl-CoA driving forces to direct the flux. We achieved high-titer (30 g/liter) and high-yield (70 to 88% of the theoretical) production of 1-butanol anaerobically, comparable to or exceeding the levels demonstrated by native producers. Without the NADH and acetyl-CoA driving forces, the Ter reaction alone only achieved about 1/10 the level of production. The engineered host platform also enables the selection of essential enzymes with better catalytic efficiency or expression by anaerobic growth rescue. These results demonstrate the importance of driving forces in the efficient production of nonnative products.

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mentioning

confidence: 98%

Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli

Shen

Lan

Dekishima

et al. 2011

Appl Environ Microbiol

565

508

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“…L(+)-lactic acid is produced commercially by lactic acid bacteria such as Lactobacillus, Lactococcus, etc., at high yield and titers from glucose and sucrose at temperatures between 30°C and 40°C (8,10). Derivatives of Escherichia coli are the only known commercial D(−)-lactic acid producers (11,12) and these also operate optimally at 40°C or lower.…”

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confidence: 99%

Evolution of D-lactate dehydrogenase activity from glycerol dehydrogenase and its utility for D-lactate production from lignocellulose

Wang

Ingram

Shanmugam

2011

Proc. Natl. Acad. Sci. U.S.A.

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Lactic acid, an attractive, renewable chemical for production of biobased plastics (polylactic acid, PLA), is currently commercially produced from food-based sources of sugar. Pure optical isomers of lactate needed for PLA are typically produced by microbial fermentation of sugars at temperatures below 40°C. Bacillus coagulans produces L(+)-lactate as a primary fermentation product and grows optimally at 50°C and pH 5, conditions that are optimal for activity of commercial fungal cellulases. This strain was engineered to produce D(−)-lactate by deleting the native ldh (L-lactate dehydrogenase) and alsS (acetolactate synthase) genes to impede anaerobic growth, followed by growth-based selection to isolate suppressor mutants that restored growth. One of these, strain QZ19, produced about 90 g L −1 of optically pure D(−)-lactic acid from glucose in <48 h. The new source of D-lactate dehydrogenase (D-LDH) activity was identified as a mutated form of glycerol dehydrogenase (GlyDH; D121N and F245S) that was produced at high levels as a result of a third mutation (insertion sequence). Although the native GlyDH had no detectable activity with pyruvate, the mutated GlyDH had a D-LDH specific activity of 0.8 μmoles min −1 ðmg proteinÞ −1 . By using QZ19 for simultaneous saccharification and fermentation of cellulose to D-lactate (50°C and pH 5.0), the cellulase usage could be reduced to 1∕3 that required for equivalent fermentations by mesophilic lactic acid bacteria. Together, the native B. coagulans and the QZ19 derivative can be used to produce either L(+) or D(−) optical isomers of lactic acid (respectively) at high titers and yields from nonfood carbohydrates.

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“…It has also been increasing in importance as a feedstock for manufacture of polylactic acid (PLA), which could be a good substitute for synthetic plastic derived from petroleum feedstock. Approximately 90% of the total lactic acid produced worldwide is by bacterial fermentation (20). The chemical synthesis of lactic acid always leads to racemic mixture, which is major disadvantage.…”

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confidence: 99%

Utilization of Molasses Sugar for Lactic Acid Production by Lactobacillus delbrueckii subsp. delbrueckii Mutant Uc-3 in Batch Fermentation

Dumbrepatil

Adsul

Chaudhari

et al. 2008

Appl Environ Microbiol

150

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Efficient lactic acid production from cane sugar molasses by Lactobacillus delbrueckii mutant Uc-3 in batch fermentation process is demonstrated. Lactic acid fermentation using molasses was not significantly affected by yeast extract concentrations. The final lactic acid concentration increased with increases of molasses sugar concentrations up to 190 g/liter. The maximum lactic acid concentration of 166 g/liter was obtained at a molasses sugar concentration of 190 g/liter with a productivity of 4.15 g/liter/h. Such a high concentration of lactic acid with high productivity from molasses has not been reported previously, and hence mutant Uc-3 could be a potential candidate for economical production of lactic acid from molasses at a commercial scale.

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Fermentation of 12% (w/v) Glucose to 1.2 m Lactate by Escherichia coli Strain SZ194 using Mineral Salts Medium

Cited by 75 publications

References 40 publications

Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli

Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli

Evolution of D-lactate dehydrogenase activity from glycerol dehydrogenase and its utility for D-lactate production from lignocellulose

Utilization of Molasses Sugar for Lactic Acid Production by Lactobacillus delbrueckii subsp. delbrueckii Mutant Uc-3 in Batch Fermentation

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