Fermentation Exhaust Gas Analysis Using Mass Spectrometry

Buckland, Barry C.; Brix, T.; Fastert, Henry; Gbewonyo, Kodzo; Hunt, Graham R.; Jain, Deepak Kumar

doi:10.1038/nbt1185-982

Cited by 54 publications

(19 citation statements)

References 5 publications

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“…Online oxygen uptake rate (and carbon dioxide production rate) have been possible to measure and calculate routinely for some time now using mass spectrometry (Buckland et al, 1985). Coupled with online dissolved oxygen measurements, this technique can also be used to measure online k L a.…”

Section: -227 Scale-up At Equal Volumetric Mass Transfer Coefficimentioning

confidence: 99%

Mixing in the Fermentation and Cell Culture Industries

Amanullah¹,

Buckland²,

Nienow

2003

Handbook of Industrial Mixing

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Section: -227 Scale-up At Equal Volumetric Mass Transfer Coefficimentioning

confidence: 99%

Mixing in the Fermentation and Cell Culture Industries

Amanullah¹,

Buckland²,

Nienow

2003

Handbook of Industrial Mixing

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“…1982 Brix et al (1988); Buckland and Fastert (1982) Distributed process control and data acquisition; calculated heat load then set limit on maximum valve stroke to avoid two sepearted cooling valves for low and high demands Bader and Petersen (1990) routinely used computers for several tasks including on-line process monitoring, control, data acquisition, and alarm scanning (Armiger and Humphrey, 1979), often with trending and graphing capabilities for measured and calculated data. These implementations achieved a primary goal of obtaining as much information as possible from fermentations, particularly those operating unattended during off-hours.…”

Section: Aspergillus Niger (Citric Acid)mentioning

confidence: 99%

Bioprocess monitoring and computer control: Key roots of the current PAT initiative

Junker

Wang

2006

Biotech & Bioengineering

113

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This review article has been written for the journal, Biotechnology and Bioengineering, to commemorate the 70th birthday of Daniel I.C. Wang, who served as doctoral thesis advisor to each of the co-authors, but a decade apart. Key roots of the current PAT initiative in bioprocess monitoring and control are described, focusing on the impact of Danny Wang's research as a professor at MIT. The history of computer control and monitoring in biochemical processing has been used to identify the areas that have already benefited and those that are most likely to benefit in the future from PAT applications. Past applications have included the use of indirect estimation methods for cell density, expansion of on-line/at-line and on-line/in situ measurement techniques, and development of models and expert systems for control and optimization. Future applications are likely to encompass additional novel measurement technologies, measurements for multi-scale and disposable bioreactors, real time batch release, and more efficient data utilization to achieve process validation and continuous improvement goals. Dan Wang's substantial contributions in this arena have been one key factor in steering the PAT initiative towards realistic and attainable industrial applications.

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“…Measured RQ values were somewhat lower for glycerol. Mass transfer coefficients were calculated using methods published previously (Buckland et al, 1985). Online measurements were monitored closely during development, especially between 50 and 150 h which was considered a key period.…”

Section: On-line Analysismentioning

confidence: 99%

Production of indole diterpenes by Aspergillus alliaceus

Junker

Walker

Connors

et al. 2006

Biotech & Bioengineering

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Production of two related indole diterpenes (differing by a dimethyl leucine side chain) by Aspergillus alliaceus was improved through several pilot scale fermentations. Media were optimized through focus primarily on initial increases, as well as mid-cycle additions, of carbon and nitrogen sources. Fermentation conditions were improved by varying ventilation conditions using various combinations of air flowrate and back-pressure set points. Production improvements were quantified based on total indole diterpene concentration as well as the ratio of the major-to-minor by-product components. Those changes with a positive substantial impact primarily on total indole diterpene concentration included early cycle glycerol shots and enhanced ventilation conditions (high air flowrate, low back-pressure). Those changes with a significant impact primarily on ratio included higher initial cerelose, soybean oil, monosodium glutamate, tryptophan, or ammonium sulfate concentrations, higher broth pH, and enhanced ventilation conditions. A few changes (higher initial glycerol and monosodium glutamate concentrations) resulted in less notable and desirable titer or ratio changes when implemented individually, but they were adopted to more fully realize the impact of other improvements or to simplify processing. Overall, total indole diterpene titers were improved at the 600 L pilot scale from 125-175 mg/L with a ratio of about 2.1 to 200-260 mg/L with a ratio of about 3.3-4.5. Thus, the ability to optimize total indole diterpene titer and/or ratio readily exists for secondary metabolite production using Aspergillus cultures.

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Fermentation Exhaust Gas Analysis Using Mass Spectrometry

Cited by 54 publications

References 5 publications

Mixing in the Fermentation and Cell Culture Industries

Mixing in the Fermentation and Cell Culture Industries

Bioprocess monitoring and computer control: Key roots of the current PAT initiative

Production of indole diterpenes by Aspergillus alliaceus

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