Fermentation Characteristics and anti-Helicobacter pylori Activity of Aqueous Broccoli Fermented by Lactobacillus plantarum MG208

Abstract

Helicobacter pylori infection causes gastrointestinal diseases such as chronic gastritis, peptic ulcers, and may lead to gastric cancer. Several studies have reported that lactobacilli present on broccoli show inhibitory activity against H. pylori. Here, we evaluated aqueous broccoli, fermented by Lactobacillus plantarum MG208, for its fermentation characteristics and anti-H. pylori activities including antibacterial activity, growth inhibition, antiadhesion, and urease inhibition. The results indicated that the fermentation characteristics changed significantly depending on the amount of aqueous broccoli used for fermentation (p <0.05). There was no significant difference between the samples before fermentation (p >0.05). However, a significant concentrationdependent difference was noted in antibacterial activity and urease inhibition (p <0.05) following the addition of aqueous broccoli. Growth inhibition in the 10 mg/mL sample was significantly higher as compared to the negative control and similar to that with amoxicillin (positive control) (p <0.05). Anti-adhesion activity of aqueous broccoli was also significantly different (p <0.05) from the negative control. Therefore, aqueous broccoli fermented by L. plantarum MG208 could prove useful as a functional diet for protection of the gastric environment against H. pylori infection.

Biopreservation and Bioactivation Juice from Waste Broccoli with Lactiplantibacillus plantarum

“…The construction of a recombinant Lactiplantibacillus plantarum strain expressing the SARS-CoV-2 spike protein can be used as a promising food-grade oral vaccine candidate against SARS-CoV-2 infection [ 15 ]. Yang et al [ 16 ] conducted a study on the effects of aqueous fermented broccoli on the chronic inflammation of the gastrointestinal tract, including gastritis, ulcers, and gastric tumors induction by the Helicobacter pylori bacteria. They showed that the presence of Lactiplantibacillus plantarum bacilli inhibits the growth of Helicobacter bacteria.…”

“…They showed that the presence of Lactiplantibacillus plantarum bacilli inhibits the growth of Helicobacter bacteria. The fermented broccoli using the Lactiplantibacillus plantarum MG208 strain may constitute a functional diet for the treatment of Helicobacter pylori infections [ 16 ]. Mousavi et al [ 17 ] confirmed that the rate of glucose transformation is faster than fructose to lactic acid transformation by Lactiplantibacillus plantarum .…”