Fenugreek (Trigonella foenum-graecum L.) Seeds Dietary Supplementation Regulates Liver Antioxidant Defense Systems in Aging Mice

Abstract

Fenugreek seeds are widely used in Asia and other places of the world for their nutritive and medicinal properties. In Asia, fenugreek seeds are also recommended for geriatric populations. Here, we evaluated for the first time the effect of fenugreek seed feed supplementation on the liver antioxidant defense systems in aging mice. The study was conducted on 12-months aged mice which were given fenugreek seed dietary supplement. We evaluated the activities of various antioxidant defense enzymes such as superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GPx), and also estimated the phenolics and free radical scavenging properties in mice liver upon fenugreek supplementation. The estimation of SOD, GPx, and GR activities in aged mice liver revealed a significant (p < 0.01) difference among all the liver enzymes. Overall, this study reveals that fenugreek seed dietary supplementation has a positive effect on the activities of the hepatic antioxidant defense enzymes in the aged mice.

Aging-Related Changes in the Ultrastructure of Hepatocytes and Cardiomyocytes of Elderly Mice Are Enhanced in ApoE-Deficient Animals

“…Mitochondria are considered the main intracellular source of superoxide anion (O 2 − ), as well as the major target of free radical attacks. ROS produced by the mitochondrial respiratory chain damage mitochondrial constituents, including proteins, lipids, and mitochondrial DNA [7][8][9][10][11].…”

Animal Nutrition and Welfare in Sustainable Production Systems

“…Fenugreek seeds are known as medicinal seeds that have several therapeutic properties such as antibacterial and anti-inflammatory [147]. They are also rich in protein, fat, carbohydrates, and minerals and contain biotin and trimethylamine, which tend to stimulate the animals' appetite [148]. Studies by Tewari et al [148] revealed that fenugreek seed dietary supplementation has a positive effect on the activities of the hepatic antioxidant defence enzymes in animals.…”

“…They are also rich in protein, fat, carbohydrates, and minerals and contain biotin and trimethylamine, which tend to stimulate the animals' appetite [148]. Studies by Tewari et al [148] revealed that fenugreek seed dietary supplementation has a positive effect on the activities of the hepatic antioxidant defence enzymes in animals. Galactomannan is the major polysaccharide found in fenugreek seeds and represents approximately 50% of the seed weight [149].…”

Untitled

“…A randomized controlled clinical trial suggested its use as adjuvant therapy to anti-diabetic drugs as it showed insignificant drop in fasting blood glucose but significant increase in fasting insulin level, significant decrease in ratio of high-to low-density lipoprotein with no notable adverse impacts on hepatic and renal functions [40] Seed dietary supplementation has a positive effect on the activities of the hepatic antioxidant defense enzymes (significant (P<0.01) difference among all the liver enzymes) in the aged mice [41] 11. Saffron 25-50 mg Neuroprotective, antidepressive and anticancer activities A double-blind, randomized and placebo-controlled trial proved significant effect on the Beck Depression Inventory (BDI) and Beck Anxiety Inventory (BAI) score [42] On liver cancer cell line QGY-7703, significant inhibition of cell growth, arrest of cell cycle in the G0/G1 phase, induction of cell apoptosis and increase in the number of senescent cells were found [43] 12.…”