Fenton reaction-driven pro-oxidant synergy of ascorbic acid and iron oxide nanoparticles in MIL-88B(Fe)

Bondarenko, Lyubov; Baimuratova, Rose; Dzeranov, Artur; Pankratov, Denis; Kicheeva, Arina; Sushko, Ekaterina; Kudryasheva, Nadezhda; Valeev, Rishat; Tropskaya, Natalya; Dzhardimalieva, Gulzhian; Kydralieva, Kamila

doi:10.1039/d4nj00963k

Cited by 1 publication

(1 citation statement)

References 95 publications

(151 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3a). This phenomenon can be attributed to the capacity of Clto enhance Fenton reactions (Bondarenko et al, 2024) induced by copper and iron cations, while not exerting the same influence on other metal cations, including Ni 2+ and Mn 2+ (Shan et al, 2016). Furthermore, the addition of MES at concentrations of 31 mmol/L and higher led to an improvement in the signal of the LNNP-based assay (Fig.…”

Section: The Effect Of Buffer Molarity and Salt Additionmentioning

confidence: 96%

Enhancing Nanozyme-Based Colorimetric Assays by Optimizing Substrate Composition

Khramtsov,

Valeev,

Eremina

et al. 2024

Preprint

View full text Add to dashboard Cite

Nanozymes, while promising alternatives to natural peroxidases in colorimetric assays, are often hindered by lower catalytic efficiencies. While various strategies exist to enhance signal intensity in nanozyme-based assays, substrate optimization remains largely underexplored. The vast majority of studies rely on standard sodium acetate buffers or commercially-sourced substrates optimized for horseradish peroxidase, neglecting the unique catalytic properties of different nanozymes. This work presents a systematic optimization of 3,3',5,5'-tetramethylbenzidine (TMB)-based substrate compositions for four common nanozymes: iron oxide, LaNiO3, Mn-doped CeO2, and platinum nanoparticles. Our findings reveal that while sodium acetate buffer is suitable for LaNiO3, alternative buffers significantly enhance signal intensity (41-68%) for the other nanozymes. Further optimization of ionic strength, organic co-solvent type and concentration, and TMB/H2O2 concentrations yielded improvements in signal intensity, analytical sensitivity, and assay time. This study also identifies common pitfalls encountered during substrate optimization and proposes potential solutions. We posit that substrate composition should be a standard optimization step in the development of nanozyme-based assays, and the use of commercially-sourced substrates with undisclosed compositions should be avoided.

show abstract

Section: The Effect Of Buffer Molarity and Salt Additionmentioning

confidence: 96%

Enhancing Nanozyme-Based Colorimetric Assays by Optimizing Substrate Composition

Khramtsov,

Valeev,

Eremina

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Fenton reaction-driven pro-oxidant synergy of ascorbic acid and iron oxide nanoparticles in MIL-88B(Fe)

Abstract: MIL-88B, a promising Fe-based 3D porous metal-organic framework (MOF) catalyst for the Fenton reaction, requires modifications to enhance its pro-oxidant activity and enable magnetic control of the sample. This study...

Cited by 1 publication

References 95 publications

Enhancing Nanozyme-Based Colorimetric Assays by Optimizing Substrate Composition

Enhancing Nanozyme-Based Colorimetric Assays by Optimizing Substrate Composition

Contact Info

Product

Resources

About