Fenofibrate inhibited pancreatic cancer cells proliferation via activation of p53 mediated by upregulation of LncRNA MEG3

Hu, Duanmin; Su, Cunjin; Jiang, Min; Shen, Yating; Shi, Aiming; Zhao, Fu-Jun; Chen, Ruidong; Shen, Zhu; Bao, Junjie; Tang, Wen

doi:10.1016/j.bbrc.2016.01.169

Cited by 85 publications

(70 citation statements)

References 25 publications

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“…LncRNA is another group of non-coding RNA involved in pancreatic cancer by regulating cell metastasis, proliferation and apoptosis by different mechanisms [50][51][52], which also plays an important role in CSCs [52][53][54]. And our profiling of PCSCs showed 34 differently expressed LncRNAs, among which, MEG3 has been shown to be able to regulate cell proliferation via activation of p53 in pancreatic cancer [53,55], while others have not been reported yet on cancers, but have numerous potential targets predicted by several LncRNA target-predicting programs. For the RNAs we are interested in, we may study them with further verification of their expression in tumor-derived PCSCs and spheroid cells from other pancreatic cancer cell line.…”

Section: Discussionmentioning

confidence: 86%

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Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

Yang¹,

Zhang²,

Tang³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Pancreatic cancer remains one of the deadliest human malignancies, the lethality of which may be attributed to the presence of pancreatic cancer stem cells (PCSCs), a small subpopulation of cells existing within pancreatic tumor with high carcinogenesis. Therefore, it is crucial to establish an efficient enrichment and culture system of PCSCs and identify the key genes involved in the regulation of PCSCs. The three-dimensional (3D) liquid suspension mammosphere culture system has been established for enrichment and culture of PCSCs in vitro as the cell spheres are likely to originate from individual cell clone, but it has been challenged because the cell spheroids could be a result of cell aggregation. Methods: We optimized the existing culture system by adding methylcellulose to create a 3D semi-solid system which prevented the non-specific aggregation. Then we identified the CSC properties of Panc-1 spheroid cells cultured by this system by detecting the genes associated with stemness and by evaluation of the tumorigenicity in vitro and in vivo through invasion, migration and xenograft experiments methods. Subsequently, we performed high-throughput sequencing (HTS) of the Panc-1 spheroid cells. Results: We confirmed the PCSCs properties and high malignancy of the Panc-1 spheroid cells enriched by our novel 3D semi-solid system both in vitro and in vivo. Hundreds of mRNA, microRNA (miRNA) and dozens of long non-coding RNA (LncRNA) were identified to be differentially regulated in PCSCs-like Panc-1 spheroid cells compared with their parental cells by HTS. Conclusions: Our results demonstrate an efficient enrichment and culture system for Panc-1 spheroid cells with the PCSCs properties. The differentially expressed genes and their targets identified by the HTS of the Panc-1 spheroid cells can serve as new potential biomarkers for pancreatic cancer diagnosis and targeted therapy.

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Section: Discussionmentioning

confidence: 86%

“…In KEGG analysis of mRNA and miRNA, the "proteoglycans in cancer" pathway is the most significant. Proteoglycans have been shown to play an important role in regulating cancer progression [55,57]. Furthermore, stemness can also be regulated by some proteoglycans [56,58].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

Yang¹,

Zhang²,

Tang³

et al. 2018

Cell Physiol Biochem

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“…LncRNAs contribute to physiological processes as well as pathological conditions, and play a variety of roles as tumor suppressors, preoperative predictors, and prognostic biomarkers [13–17]. A previous study has demonstrated that upregulation of chromatin-interacting lncRNA, MEG3, inhibits PCa cell proliferation via the activation of p53 [18]. Additionally, lncRNA-NUTF2P3-001 contributes to PCa proliferation and invasion by derepressing the miR-3923/KRAS pathway [19].…”

Section: Discussionmentioning

confidence: 99%

Plasma and tumor levels of Linc-pint are diagnostic and prognostic biomarkers for pancreatic cancer

Zhang

Chen

et al. 2016

Oncotarget

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Long intergenic non-protein coding RNA, p53 induced transcript (Linc-pint) is a long noncoding RNA (lncRNA) that regulates tumor cell viability and proliferation. We used qRT-PCR and RNA FISH analysis to evaluate Linc-pint levels in the plasma and tumor tissues of pancreatic cancer (PCa) patients. Our data demonstrate that Linc-pint expression is lower in plasma samples from PCa patients than from healthy individuals, and indicate that plasma Linc-pint levels are more sensitive than CA19-9 for detecting PCa. Our data also show that Linc-pint levels are lower in PCa tumors than in adjacent tissues, carcinoma of the ampulla of Vater (CAV) and cholangiocarcinoma (CCA), and suggest that Linc-pint could be used for distinguishing the cause of malignant obstructive jaundice. Low plasma Linc-pint levels correlate with tumor recurrence, while low tumor Linc-pint levels correlate with poor prognosis for PCa patients after pancreatectomy. These results thus indicate that low plasma Linc-pint expression could serve as a minimally invasive biomarker for early PCa detection, and that low Linc-pint levels in PCa tumors could be used for predicting patient prognosis.

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“…Maternally Expressed Gene 3 (MEG3), a tumor suppressor, is involved in the development and progression of many cancers [47–49]. MEG3 is an imprinted gene located at chromosome 14q32 that encodes a 1.6-kb noncoding RNA from the maternal allele [50].…”

Section: Lncrnas In Hncmentioning

confidence: 99%

Long noncoding RNAs in head and neck cancer

Cao

Gong

et al. 2016

Oncotarget

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Head and neck cancers (HNCs) include a series of malignant tumors arising in epithelial tissues, typically oral cancer, laryngeal cancer, nasopharynx cancer and thyroid cancer. HNCs are important contributors to cancer incidence and mortality, leading to approximately 225,100 new patients and 77,500 deaths in China every year. Determination of the mechanisms of HNC carcinogenesis and progression is an urgent priority in HNC treatment. Long noncoding RNAs (lncRNAs) are noncoding RNAs longer than 200 bps. lncRNAs have been reported to participate in a broad scope of biological processes, and lncRNA dysregulation leads to diverse human diseases, including cancer. In this review, we focus on lncRNAs that are dysregulated in HNCs, summarize the latest findings regarding the function and molecular mechanisms of lncRNAs in HNC carcinogenesis and progression, and discuss the clinical application of lncRNAs in HNC diagnosis, prognosis and therapy.

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Fenofibrate inhibited pancreatic cancer cells proliferation via activation of p53 mediated by upregulation of LncRNA MEG3

Cited by 85 publications

References 25 publications

Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

Transcriptome Profiling of Panc-1 Spheroid Cells with Pancreatic Cancer Stem Cells Properties Cultured by a Novel 3D Semi-Solid System

Plasma and tumor levels of Linc-pint are diagnostic and prognostic biomarkers for pancreatic cancer

Long noncoding RNAs in head and neck cancer

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