Fenestrae in the Rough Endoplasmic Reticulum of the Exocrine Pancreatic Cells

Orci, Lelio; Perrelet, Alain; Like, Arthur A.

doi:10.1083/jcb.55.1.245

Cited by 44 publications

(6 citation statements)

References 7 publications

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“…Later, they were detected in a wide range of species and cell types ( Lieberman, 1971 ; Orci et al. , 1971 , 1972 ; Waugh et al. , 1973 ; Tani et al.…”

Section: Introductionmentioning

confidence: 99%

Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells

Puhka

Joensuu

Vihinen

et al. 2012

MBoC

162

154

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“…Later, they were detected in a wide range of species and cell types ( Lieberman, 1971 ; Orci et al. , 1971 , 1972 ; Waugh et al. , 1973 ; Tani et al.…”

Section: Introductionmentioning

confidence: 99%

Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells

Puhka

Joensuu

Vihinen

et al. 2012

MBoC

162

154

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“…Figures 9 to 11 represent fractured areas with ER and mitochondrions of the same sample as Figure 2. The arrows point to porous structures found in the ER membranes fenestrae; Orci et al, 1972!. In Figure 9, the ER is fractured perpendicular to the membranes in the middle of the image, and fractured in parallel to the membranes at the right-hand side.…”

Section: Resultsmentioning

confidence: 99%

Cryo-Fracturing and Cryo-Planing for In-Lens Cryo-SEM, Using a Newly Designed Diamond Knife

Walther

2003

Microsc Microanal

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High-pressure frozen rat pancreas tissue samples were cryo-fractured and cryo-sectioned with a diamond knife in the microtome of a freeze-etching device. The bulk fracture faces and block faces were investigated in the frozen-hydrated state by use of a cryo-stage in an in-lens SEM. With this combination, relevant biological structures can be investigated with a few nanometers resolution in a near lifelike state, preventing the artifacts of conventional fixation techniques. Compared to TEM replica techniques, the presented method is more versatile since no replica cleaning is necessary. Additional structures can be made visible by controlled sublimation of ice, leading to a better understanding of the three-dimensional organization of organelles, such as the endoplasmic reticulum.

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“…The macroconidia of F. solani, however, have endoplasmic reticulum that is in the form of stacks of fenestrated cisternae. Fenestrated endoplasmic reticulum is generally a characteristic of very active cells such as hepatocytes (Orci et al 1971;Tewari and Malhotra 1974) and exocrine pancreatic cells (Orci et a[. 1972), and its occurrence in these spores was unexpected.…”

Section: Discussionmentioning

confidence: 96%

Fine structure of the macroconidia of Fusarium solani

Tewari¹,

Skoropad²

1975

Can. J. Bot.

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TEWARI, J. P., and W. P. SKOROPAD. 1975. Fine structure of the macroconidia of Fuscrrictm solrrni. Can. J . Bot. 53: 2134-2146. Ultrastructure of the macroconidia of Frrsarilrm solani as visualized by transmission (ultrathin sectioning and freeze-etching) and scanning electron microscopy is described. The cell wall has four layers. The innermost layer is electron-lucid followed by an electron-dense layer. The next outer layer is spongy in appearance followed by a superficial layer consisting of fine filamentous processes. Freeze-etch replicas of conidia directly removed from the sporodochia and still suspended in the mucilaginous material (in which they are produced) frequently show the conidia connected by the superficial filamentous processes in the cell wall. This agglutination of the conidia is likely to increase the inoculum potential of this pathogen at the sites of infection. Structure of various membrane systems in the cells is described. The endoplasmic reticulum is fairly extensive and fenestrated. Thin sections of routinely fixed conidia show myelin figures. However, such structures were not seen in replicas of conidia that were freeze-etched without use of chemical fixation or cryoprotection. TEWARI, J. P., et W. P. SKOROPAD. 1975. Fine structure of the macroconidia of Frrsrrrirrm solrtni. Can. J . Bot. 53: 2134-2146. Les auteurs decrivent I'ultrastructure des macroconidies de Frisnrirrm solani en microscopic electronique B transmission (sections ultraminces et cryodecapage) et a balayage. La paroi cellulaire comporte quatre couches. La couche la plus interne est translucide aux electrons et est suivie par une couche dense aux electrons. La couche suivante est d'apparence spongieuse et est suivie par une couche superficielle constitute de fines protuberances filamenteuses.Des empreintes cyodecapees de conidies prelevees directement de la sporodochie et encore suspendues dans le materiel mucilagineux (dans lequel elles sont produites) montrent frkquemment des conidies attachees I'une a I'autre par les protuberances filamenteuses de leur paroi cellulaire. Cette agglutination des conidies est susceptible d'augmenter le potentiel infectieux de ce pathogkne aux points de contact avec l'h6te. Les auteurs decrivent les differents systkmes membranaires dans les cellules. Le reticulum endoplasmique est fenestrk et passablement Ctendu. Des sections minces de conidies fixees de f a~o n routinikre montrent des figures de myeline. Cependant, ces mZmes structures n'ont pu Ztre mises en evidence dans les empreintes de conidies soumises au cryodecapage sans fixation chimique prealable ou sans cryoprotection.[Traduit par le journal]

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Fenestrae in the Rough Endoplasmic Reticulum of the Exocrine Pancreatic Cells

Cited by 44 publications

References 7 publications

Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells

Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells

Cryo-Fracturing and Cryo-Planing for In-Lens Cryo-SEM, Using a Newly Designed Diamond Knife

Fine structure of the macroconidia of Fusarium solani

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