Femtosecond Near-Infrared Laser Pulses Elicit Generation of Reactive Oxygen Species in Mammalian Cells Leading to Apoptosis-like Death

Tirlapur, Uday K.; König, Karsten; Peuckert, Christiane; Krieg, Reimar; Kj, Halbhuber

doi:10.1006/excr.2000.5082

Cited by 189 publications

(164 citation statements)

References 45 publications

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“…Tirlapur et al (8) have reported evidence indicating that TPLSM at 800 nm caused apoptosis in PtK2 cells when average powers exceeded 7 mW. Using a similar TdT end-labeling assay, we were unable to find any evidence for induction of apoptosis in RBL cells by TPLSM for laser powers ranging from 5 to 17 mW at 740 nm.…”

contrasting

confidence: 67%

“…Oehring et al (7) have reported deformations in the organization of mitochondrial inner membranes resulting in impaired cell division of CHO-K1 cells with ultrashort 800 nm pulses at powers exceeding 2 mW. Tirlapur et al (8) have reported recently that massive oxidative stress leading to apoptosis can occur in PtK2 cells irradiated with 800 nm, 170 fs laser pulses with average powers exceeding 7 mW. On the other hand, Hockberger et al (5), working at 1047 nm, did not detect H 2 O 2 production with laser powers as high as 20 mW, and Squirrell et al (6) were able to maintain hamster embryo viability under prolonged imaging at 1047 nm with 13 mW average laser power.…”

Section: Introductionmentioning

confidence: 99%

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Reduction in DNA Synthesis During Two-photon Microscopy of Intrinsic Reduced Nicotinamide Adenine Dinucleotide Fluorescence¶

Nichols¹,

Barth²,

Nichols³

2005

Photochem Photobiol

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Two-photon laser scanning microscopy (TPLSM) of endogenous reduced nicotinamide adenine dinucleotide (NAD(P)H) provides important information regarding the cellular metabolic state. When imaging the punctate mitochondrial fluorescence originating from NAD(P)H in a rat basophilic leukemia (RBL) cell at low laser powers, no morphological changes are evident, and photobleaching is not observed when many images are taken. At higher powers, mitochondrial NAD(P)H fluorescence bleaches rapidly. To assess the limitations of this technique and to quantify the extent of photodamage, we have measured the effect of TPLSM on DNA synthesis. Although previous reports have indicated a threshold power for ''safe'' two-photon imaging, we find the laser power to be an insufficient indicator of photodamage. A more meaningful metric is a two-photon-absorbed dose that is proportional to the number of absorbed photon pairs. A temporary reduction of DNA synthesis in RBL cells occurs whenever a threshold dose of approximately 2 3 10 53 photon 2 cm 24 s 21 is exceeded. This threshold is independent of laser intensity when imaging with average powers ranging from 5 to 17 mW at 740 nm. Beyond this threshold, the extent of the reduction is intensity dependent. DNA synthesis returns to control levels after a recovery period of several hours.

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contrasting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Reduction in DNA Synthesis During Two-photon Microscopy of Intrinsic Reduced Nicotinamide Adenine Dinucleotide Fluorescence¶

Nichols¹,

Barth²,

Nichols³

2005

Photochem Photobiol

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“…A few of the first studies using femtosecond lasers observed the decreased cloning efficiency of cells restrained by optical traps [37][38][39] or imaged with two-photon microscopes [40]. Some cells become giant cells with multiple nuclei [41], and others undergo apoptosis because of laser-generated reactive oxygen species [42]. However, proteins involved in recognizing and repairing DNA are also present after surgery to mitigate damage [43].…”

Section: Experimental Backgroundmentioning

confidence: 99%

Surgical applications of femtosecond lasers

Chung¹,

Mazur

2009

Journal of Biophotonics

231

136

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Femtosecond laser ablation permits non‐invasive surgeries in the bulk of a sample with submicrometer resolution. We briefly review the history of optical surgery techniques and the experimental background of femtosecond laser ablation. Next, we present several clinical applications, including dental surgery and eye surgery. We then summarize research applications, encompassing cell and tissue studies, research on C. elegans, and studies in zebrafish. We conclude by discussing future trends of femtosecond laser systems and some possible application directions. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…The possibility of innate cellular proteins that can directly or nonlinearly response to photons for Ca 2+ modulation is quite attractive and may exist. It should be noted that significant physiological and molecular changes can be induced by femtosecond laser in previous studies on photodamage during multiphoton microscopy [110][111][112][113][114]. In our recent experiments, it was found that moderate and controllable Ca 2+ release could be also activated by multiphoton excitation which implied possible innate molecules inside cells that could directly response to photons for Ca 2+ modulation.…”

Section: Discussionmentioning

confidence: 70%

“…In turn, released ROS can also activate molecules like IP 3 Rs and RyRs to facilitate Ca 2+ release from internal stores [21]. Furtherly, apoptosis may be initiated by overloading mitochondria with Ca 2+ to release cytochrome c through the PTP [19,109], where ROS play a role in membrane barrier dysfunction, structural deformations and fragmentation of the nuclei and DNA strand breaks [110]. Therefore, ROS can be affected as a response to femtosecond laser and in turn induce calcium increase and thus influence relative signal pathways and cellular processes like cell death [111].…”

Section: Subsequent Cellular Responses To Femtosecond Laser Induced Cmentioning

confidence: 99%

Regulation of Cellular Molecular Signaling by Light (Invited Paper)

Cheng¹,

Zhu²,

He³

2015

PIER

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Abstract-Laser technology has been promoting various microscopy methods and thus making great progresses in life science. Further than contribution to "seeing is believing", lasers have also demonstrated their capacity of manipulating cells and even molecular signaling. Specifically, with advances of lasers and combination with other techniques, recent reports show that cell calcium ion, a universal intra-and inter-cellular messenger, can be modulated by lasers at different levels of biological organization from organelle to tissue. It is very encouraging that laser irradiation can activate or control plenty of corresponding cell processes and functions by regulating cell calcium signaling pathways, with promising potential in both scientific research and clinical application. In this paper, optical techniques for regulation of cell calcium signaling are specifically reviewed. Most methods need exogenous chemicals or genetic materials to convert incident photon into stimulation that cells can response with specific molecular dynamics. The only all-optical approach is achieved by nonlinear excitation with femtosecond laser, despite lack of specificity and controllability, providing possibility of a totally noninvasive method without any biochemical materials and thus further potential clinical application in human beings. The developments and techniques of those methods are introduced and explained, with analysis on their properties and current challenges. Potential applications and prospective development are also discussed. Researchers on biophotonics and related biological fields can benefit from this review. It also provides a systematic reference to doctors and researchers who are working on practical application of those methods.

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Femtosecond Near-Infrared Laser Pulses Elicit Generation of Reactive Oxygen Species in Mammalian Cells Leading to Apoptosis-like Death

Cited by 189 publications

References 45 publications

Reduction in DNA Synthesis During Two-photon Microscopy of Intrinsic Reduced Nicotinamide Adenine Dinucleotide Fluorescence¶

Reduction in DNA Synthesis During Two-photon Microscopy of Intrinsic Reduced Nicotinamide Adenine Dinucleotide Fluorescence¶

Surgical applications of femtosecond lasers

Regulation of Cellular Molecular Signaling by Light (Invited Paper)

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