Femtosecond Laser-Assisted Retinal Imaging and Ablation: Experimental Pilot Study

Hild, Matthias; Krause, Matthias; Riemann, Iris; Mestres, Pedro; Toropygin, S.G.; Löw, U.; Bruckner, K.; Seitz, Berthold; Jonescu-Cuypers, Christian P.; König, Karsten

doi:10.1080/02713680801956452

Cited by 11 publications

(3 citation statements)

References 30 publications

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“…Femtosecond pulse lasers have been proved to be a precision tool for ablating cells and subcellular structures during the last few years [44,45]. In combination of this laser ablation system, MPM can be utilized to conduct imaging on biological tissues clearly and select regions for multiphoton ablation.…”

Section: Discussionmentioning

confidence: 99%

Label-free detection of brain invasion in meningiomas by multiphoton microscopy

Fang

Cai

et al. 2018

Laser Phys. Lett.

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Brain invasion in meningiomas relates to the poor prognosis. Therefore, accurate detection and treatment of brain invasion are of particular importance. In this study, multiphoton microscopy (MPM) was introduced for label-free detection of brain invasion in meningiomas by the combination of the two-photon excited fluorescence imaging, second-harmonic generation imaging and spectral imaging. Results show that MPM has the ability to morphologically and spectrally identify brain invasion in meningiomas. What is more, the intensity ratios of the cerebral cortex over meningiomas at 475 nm, 511 nm and 540 nm were extracted as quantitative indicators for differentiating meningioma from surrounding normal cerebral cortex tissue. Together with custom-developed image processing algorithms, MPM could further identify the tumor boundary. With miniaturization and optimization of probes and optical fiber for in vivo imaging, combined with a laser ablation system, MPM would be used in an operating room for both the diagnosis and treatment of brain invasion in meningiomas.

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Section: Discussionmentioning

confidence: 99%

Label-free detection of brain invasion in meningiomas by multiphoton microscopy

Fang

Cai

et al. 2018

Laser Phys. Lett.

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“…Over the past decade, powerful femtosecond pulse lasers have been demonstrated in use as precise cutting tools for ablating cells and subcellular structures [37,38]. Combined with laser ablation systems, MPM offers the ability to clearly image biological tissues and simultaneously select areas for multiphoton ablation.…”

Section: Discussionmentioning

confidence: 99%

Multiphoton imaging of low grade, high grade intraepithelial neoplasia and intramucosal invasive cancer of esophagus

Jiang

Kang

et al. 2017

Laser Phys. Lett.

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Esophageal squamous cell carcinoma (ESCC) is devastating because of its aggressive lymphatic spread and clinical course. It is believed to occur through low-grade intraepithelial neoplasia (LGIN), high-grade intraepithelial neoplasia (HGIN), and intramucosal invasive cancer (IMC) before transforming to submucosal cancer. In particular, these early lesions (LGIN, HGIN and IMC), which involve no lymph node nor distant metastasis, can be cured by endoscopic treatment. Therefore, early identification of these lesions is important so as to offer a curative endoscopic resection, thus slowing down the development of ESCC. In this work, spectral information and morphological features of the normal esophageal mucosa are first studied. Then, the morphological changes of LGIN, HGIN and IMC are described. Lastly, quantitative parameters are also extracted by calculating the nuclear-to-cytoplasmic ratio of epithelial cells and the pixel density of collagen in the lamina propria. These results show that multiphoton microscopy (MPM) has the ability to identify normal esophageal mucosa, LGIN, HGIN and IMC. With the development of multiphoton endoscope systems for in vivo imaging, combined with a laser ablation system, MPM has the potential to provide immediate pathologic diagnosis and curative treatment of ESCC before the transformation to submucosal cancer in the future.

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“…The advantages of our combined method for schwannoma tissues. In recent years, the femtosecond pulse laser has proven to be a fine cutting tool for the resection of cells or subcellular structures [21,22]. With the laser ablation system, MPM technology is capable of clearly displaying the biological tissues while excising the pathological tissues.…”

Section: Diagnostic Features Identifiable In Both Hande and This Comb...mentioning

confidence: 99%

A pilot study of using multiphoton microscopy to diagnose schwannoma

Fang

Wang

et al. 2019

J. Phys. D: Appl. Phys.

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Schwannoma is a kind of slow-growing and rare neoplasm which poses diagnostic challenges owing to its clinical silence at the presentation period. Here, a schwannoma tumor was studied by multiphoton microscopy (MPM). Results indicate that MPM is capable of identifying a schwannoma tumor without the need for labels. Based on the two-photon excited fluorescence signals from cells and blood vessels and second harmonic generation signals from collagens, several significant features for the identification of schwannoma were visualized, including the Antoni A region, Antoni B region, pericellular collagen pattern, hyalinized vessels and hyalinized stroma. These conclusions highlight the potential of MPM taken as a diagnostic method for label-free identification of schwannoma tumors by these histopathologic characteristics.

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Femtosecond Laser-Assisted Retinal Imaging and Ablation: Experimental Pilot Study

Abstract: Non-amplified femtosecond lasers may allow precise surgery controlled by fast high-resolution imaging of the target.

Cited by 11 publications

References 30 publications

Label-free detection of brain invasion in meningiomas by multiphoton microscopy

Label-free detection of brain invasion in meningiomas by multiphoton microscopy

Multiphoton imaging of low grade, high grade intraepithelial neoplasia and intramucosal invasive cancer of esophagus

A pilot study of using multiphoton microscopy to diagnose schwannoma

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