Femtosecond Laser-Assisted Optoporation for Drug and Gene Delivery into Single Mammalian Cells

Soman, Pranav; Zhang, Wande; Umeda, Aiko; Zhang, Zhiwen Jonathan; Chen, Shaochen

doi:10.1166/jbn.2011.1295

Cited by 28 publications

(20 citation statements)

References 17 publications

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“…Recently, there has been considerable interest in the optical transfection of cells using ultrafast pulsed light [15][16][17][18][19][20][21][22][23] because this approach offers selective targeting and higher efficiency and viability (.90% reported in vitro 22 ) than other methods. Furthermore, femtosecond (fs) near-infrared (NIR) laser-based transfection has been shown to be safe, resulting in high efficiency and high survival rates (93%) of optoporated embryos during development 20 , as well as suitable for in vivo gene delivery 24 .…”

Section: Introductionmentioning

confidence: 99%

Optical delivery of multiple opsin-encoding genes leads to targeted expression and white-light activation

et al. 2015

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In photodegenerative diseases such as retinitis pigmentosa (RP) and age-related macular degeneration (AMD), progressive loss of vision occurs as a result of degeneration of the periphery of the retina and the macula, respectively. Current optogenetic stimulation-based approaches to vision restoration offer the advantages of cellular specificity, high resolution, and minimal invasiveness over electrode arrays; however, the clinical translation of optogenetic activation suffers from the lack of a method for the delivery of opsins into spatially targeted regions of a retina that has degenerated. Non-targeted opsin delivery through viral or non-viral methods to non-photodegenerated retinal areas will perturb these already functioning retinal regions. Furthermore, viral methods are subject to limitations on the delivery of large plasmids, such as fusion constructs of multiple spectrally separated opsins (e.g., channelrhodopsin-2 (ChR2), chimeric opsin variants (C1V1), ReaChR), which can provide higher photo-excitability than can a single narrow-band opsin under ambient light conditions. Here, we report the ultrafast near-infrared laser-based spatially targeted transfection of single and multiple opsins and present a comparison with the opsin expression distribution achieved using another non-viral, but non-targeted, transfection method, lipofection. Functional evaluation of cells transfected with multiple opsins using the laser method revealed a significantly higher white-light-induced photocurrent than in cells expressing a single opsin (ChR2). The laser-assisted targeted delivery of multiple opsin-encoding genes to the peripheral retina/macula is ideal for sensitizing retinal areas that have degenerated, thus paving the way toward the restoration of lost vision in RP/AMD patients.

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Section: Introductionmentioning

confidence: 99%

Optical delivery of multiple opsin-encoding genes leads to targeted expression and white-light activation

et al. 2015

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“…11 Apart from the above, click chemistry including Diels-Alder reactions and Thiolyne reactions have been employed to synthesize polyphenylene dendrimers. [12][13][14][15][16][17][18] Since then, based on the previous synthetic methods, in order to endow more features to new-style macromolecules, some researchers also synthesized diverse metal (non-metal) as well as dual-core and multi-core dendritic compounds with some functional groups. [19][20][21][22] …”

Section: New-style Methodsmentioning

confidence: 99%

Advances in Applications of Dendritic Compounds

Ma²,

Deng³

et al. 2013

j. nanosci. nanotech.

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Dendritic compounds (so-called dendrimers) with nano-scale represent unique symmetric and spherical structures. They are usually prepared through two fundamental methods based on molecular level which involved the convergent method and divergent method, although the synthetic methods have a tendency towards the diversity and functionalization. The outershell of dendrimers with rich peripheral reactive sites are easily modified by small functional molecules. Moreover, the higher generation dendrimers also possess more exposed functional groups on the surface prior to the previous one and they are much easily customized for much more applications. Consequently, based on previous researches, this review summarized wide applications of dendritic compounds in many fields which were investigated in detail, including gene vector, drug carrier, catalysis, sensor industry, photoelectric material, etc. Dendrimers provide promising tools for the cellular delivery of molecular cargos ranging in size from small molecules and peptides to proteins and DNA. More importantly, it is necessary to explore new synthetic methods and undiscovered applications of new dendrimers.

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“…Biophysical energy such as electric fields, magnetic fields, ultrasound and mechanical forces, light and temperature gradients can act as enhancers for drug delivery. Technique-specific terms have been associated with each form of physical energy such as electroporation [37, 38] for electric current, iontophoresis for electric potential, magnetoporation [39, 40] for magnetic field, sonoporation [41, 42], mechanoporation [43] and phonophoresis [44, 45] for ultrasound, optoporation [46, 47] for pulsed light and thermoporation [48, 49] for temperature, respectively (Figure 1). Table 1 summarizes all of the different types of physical energy used in this review for porating the cells.…”

Section: Introductionmentioning

confidence: 99%