Femtomolar endogenous adenosine triphosphate-responded photoelectrochemical biosensor based on Au@Cu2O core-shell nanocubes for the ultrasensitive determination of Escherichia coli O157:H7 in foods

Hu, Shuhao; Liu, Yuting; Liu, Liu; Yu, Zhenzhong; Gan, Ning

doi:10.1016/j.aca.2023.341868

Cited by 7 publications

(1 citation statement)

References 42 publications

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“…Photoelectrochemical (PEC) biosensors are an integrated tool based on the combination of photoelectrochemical processes and biorecognition processes that can be used to convert analyses into recognizable signals through molecular interactions . PEC biosensors mainly include two processes, one is photoelectric conversion of photoactive materials, and the other is conversion of biochemical or chemical information into electrical signals by biomolecules through various mechanisms. , To date, various photoelectric biosensor strategies have been proposed for the detection of the METTL3/METTL14 complex enzymes. For example, using MoSe 2 /BiOI as a photoactive material, the METTL3/METTL14 protein was detected by a catalytic hairpin self-assembly and alkaline phosphatase (ALP)-catalyzed L -ascorbic acid-2-phosphate (AAP) in situ generation of electron donor AA signal amplification strategy .…”

Section: Introductionmentioning

confidence: 99%

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Zhang,

Zhou

et al. 2024

Anal. Chem.

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The OPECT biosensing platform, which connects optoelectronics and biological systems, offers significant amplification and more possibilities for research in biological applications. In this work, a homogeneous organic photoelectrochemical transistor (OPECT) biosensor based on a Bi 2 S 3 /Bi 2 MoO 6 heterojunction was constructed to detect METTL3/METTL14 protein activity. The METTL3/METTL14 complex enzyme was used to catalyze adenine (A) on an RNA strand to m 6 A, protecting m 6 A-RNA from being cleaved by an E. coli toxin (MazF). Alkaline phosphatase (ALP) catalyzed the conversion of Na 3 SPO 3 to H 2 S through an enzymatic reaction. Due to the adoption of the strategy of no fixation on the electrode, the generated H 2 S was easy to diffuse to the surface of the ITO electrode. The Bi 2 S 3 /Bi 2 MoO 6 heterojunction was formed in situ through a chemical replacement reaction with Bi 2 MoO 6 , improving photoelectric conversion efficiency and realizing signal amplification. Based on this "signal on" mode, METTL3/METTL14 exhibited a wide linear range (0.00001−25 ng/μL) between protein concentration and photocurrent intensity with a limit of detection (LOD) of 7.8 fg/μL under optimal experimental conditions. The applicability of the developed method was evaluated by investigating the effect of four plasticizers on the activity of the METTL3/METTL14 protein, and the molecular modeling technique was employed to investigate the interaction between plasticizers and the protein.

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Section: Introductionmentioning

confidence: 99%

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Zhang,

Zhou

et al. 2024

Anal. Chem.

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Detection of foodborne pathogens in contaminated food using nanomaterial-based electrochemical biosensors

Flores-Ramírez,

González-Estrada,

Chacón-López

et al. 2024

Analytical Biochemistry

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From engineered photoactive materials to detection signal amplification strategies in photoelectrochemical biosensing of pathogens: New horizons and perspectives

Xu,

et al. 2024

Chemical Engineering Journal

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Femtomolar endogenous adenosine triphosphate-responded photoelectrochemical biosensor based on Au@Cu2O core-shell nanocubes for the ultrasensitive determination of Escherichia coli O157:H7 in foods

Cited by 7 publications

References 42 publications

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Detection of foodborne pathogens in contaminated food using nanomaterial-based electrochemical biosensors

From engineered photoactive materials to detection signal amplification strategies in photoelectrochemical biosensing of pathogens: New horizons and perspectives

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Femtomolar endogenous adenosine triphosphate-responded photoelectrochemical biosensor based on Au@Cu2O core-shell nanocubes for the ultrasensitive determination of Escherichia coli O157:H7 in foods

Cited by 7 publications

References 42 publications

Enzymatically Mediated In Situ Generation of Z-Scheme Bi2S3/Bi2MoO6 Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Enzymatically Mediated In Situ Generation of Z-Scheme Bi2S3/Bi2MoO6 Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Detection of foodborne pathogens in contaminated food using nanomaterial-based electrochemical biosensors

From engineered photoactive materials to detection signal amplification strategies in photoelectrochemical biosensing of pathogens: New horizons and perspectives

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Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection

Enzymatically Mediated In Situ Generation of Z-Scheme Bi₂S₃/Bi₂MoO₆ Heterojunction-Based Organic Photoelectrochemical Transistor for METTL3/METTL14 Detection