Femtomolar Detection of Dengue Virus DNA with Serotype Identification Ability

Chowdhury, Ankan Dutta; Ganganboina, Akhilesh Babu; Nasrin, Fahmida; Takemura, Kenshin; Doong, Ruey‐an; Utomo, Doddy Irawan Setyo; Lee, Jaewook; Khoris, Indra Memdi; Park, Enoch Y.

doi:10.1021/acs.analchem.8b01802

Cited by 59 publications

(40 citation statements)

References 38 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the cost of reagents and time required could be considered drawbacks of these methods. Also, although quantification of a disease can be accomplished, the selectivity may be a challenge in cases of cross-reactivity with other infectious diseases ( Chowdhury et al, 2018 ; Xiang et al, 2018 ). These facts call for the development of new, fast, and accurate diagnostic tools for the detection of pathogenic viruses.…”

Section: Methods Of Detection Of Viral Pathogens Of Humansmentioning

confidence: 99%

“…Nitrogen alters the electronic characteristics and amplifies the electrochemical activity, whereas sulfur creates numerous anchoring sites for noble nanoparticle attachment. In addition, the presence of edge carboxylic groups on GQDs allows antibody conjugation ( Chowdhury et al, 2019 , 2018 ). The tunable fluorescence properties, inherent redox activity, chemiluminescence, and catalytic effect of GQDs make them promising materials for optical, electrochemical, and electrochemiluminescence (ECL) biosensing applications with enhanced selectivity and sensitivity.…”

Section: Carbon-based Nanomaterialsmentioning

confidence: 99%

“…MB was used as a redox indicator for the DPV measurements. This technique is also an example of DNA hybridization detection ( Chowdhury et al, 2018 ).…”

Section: Graphene-based Materials In Virus Sensingmentioning

confidence: 99%

See 2 more Smart Citations

Human virus detection with graphene-based materials

Vermisoglou

Panáček

Jayaramulu

et al. 2020

Biosensors and Bioelectronics

153

117

View full text Add to dashboard Cite

Our recent experience of the COVID-19 pandemic has highlighted the importance of easy-to-use, quick, cheap, sensitive and selective detection of virus pathogens for the efficient monitoring and treatment of virus diseases. Early detection of viruses provides essential information about possible efficient and targeted treatments, prolongs the therapeutic window and hence reduces morbidity. Graphene is a lightweight, chemically stable and conductive material that can be successfully utilized for the detection of various virus strains. The sensitivity and selectivity of graphene can be enhanced by its functionalization or combination with other materials. Introducing suitable functional groups and/or counterparts in the hybrid structure enables tuning of the optical and electrical properties, which is particularly attractive for rapid and easy-to-use virus detection. In this review, we cover all the different types of graphene-based sensors available for virus detection, including, e.g., photoluminescence and colorimetric sensors, and surface plasmon resonance biosensors. Various strategies of electrochemical detection of viruses based on, e.g., DNA hybridization or antigen-antibody interactions, are also discussed. We summarize the current state-of-the-art applications of graphene-based systems for sensing a variety of viruses, e.g., SARS-CoV-2, influenza, dengue fever, hepatitis C virus, HIV, rotavirus and Zika virus. General principles, mechanisms of action, advantages and drawbacks are presented to provide useful information for the further development and construction of advanced virus biosensors. We highlight that the unique and tunable physicochemical properties of graphene-based nanomaterials make them ideal candidates for engineering and miniaturization of biosensors.

show abstract

Section: Methods Of Detection Of Viral Pathogens Of Humansmentioning

confidence: 99%

Section: Carbon-based Nanomaterialsmentioning

confidence: 99%

See 1 more Smart Citation

Human virus detection with graphene-based materials

Vermisoglou

Panáček

Jayaramulu

et al. 2020

Biosensors and Bioelectronics

153

117

View full text Add to dashboard Cite

show abstract

“…The high surface area of nanoparticles was exploited for loading of a high amount of capture DNA. Chowdhury et al had detected Dengue virus DNA using nanocomposites of gold nanoparticles (AuNP) with nitrogen and sulfur co-doped graphene quantum dots (N,S-GQDs@AuNP) [ 56 ]. First, N,S-GQDs@AuNP were coated with a capture DNA (polydA) using Au-thiol bond formation.…”

Section: Signal Amplification Approaches For the Dna/rna Electrochmentioning

confidence: 99%

DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment

Santhanam

Algov

Alfonta

2020

Sensors

View full text Add to dashboard Cite

Pandemics require a fast and immediate response to contain potential infectious carriers. In the recent 2020 Covid-19 worldwide pandemic, authorities all around the world have failed to identify potential carriers and contain it on time. Hence, a rapid and very sensitive testing method is required. Current diagnostic tools, reverse transcription PCR (RT-PCR) and real-time PCR (qPCR), have its pitfalls for quick pandemic containment such as the requirement for specialized professionals and instrumentation. Versatile electrochemical DNA/RNA sensors are a promising technological alternative for PCR based diagnosis. In an electrochemical DNA sensor, a nucleic acid hybridization event is converted into a quantifiable electrochemical signal. A critical challenge of electrochemical DNA sensors is sensitive detection of a low copy number of DNA/RNA in samples such as is the case for early onset of a disease. Signal amplification approaches are an important tool to overcome this sensitivity issue. In this review, the authors discuss the most recent signal amplification strategies employed in the electrochemical DNA/RNA diagnosis of pathogens.

show abstract

“…The biosensor sensitivity is of great importance to detect pathogens in environmental, agrifood, or biological samples because pathogens are typically present in very low concentrations. Therefore, various diagnostic assays using functionalized nanoparticles to amplify the signal and boost the sensitivity have been reported in recent years [25][26][27][28]. Herein, we propose to evaluate the sensitivity increase of MELISA for Influenza A detection via the nucleoprotein A, using immunomagnetic beads for the capture step and biotin-coated nanoparticles (biotin-NP) to enhance the signal at the detection step.…”

Section: Introductionmentioning

confidence: 99%

Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay

et al. 2020

View full text Add to dashboard Cite

This study reports the development of a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the panreactive detection of the Influenza A virus. The assay combines immunomagnetic beads and biotin-nanoparticle-based detection to quantify a highly conserved viral nucleoprotein in virus lysates. At the capture step, monoclonal antibody-coated magnetic microbeads were used to bind and concentrate the nucleoprotein in samples. The colorimetric detection signal was amplified using biotinylated silica nanoparticles (NP). These nanoparticles were functionalized on the surface with short DNA spacers bearing biotin groups by an automated supported synthesis method performed on nano-on-micro assemblies with a DNA/RNA synthesizer. A biotin-nanoparticle and immunomagnetic bead-based assay was developed. We succeeded in detecting Influenza A viruses directly in the lysis buffer supplemented with 10% saliva to simulate the clinical context. The biotin-nanoparticle amplification step enabled detection limits as low as 3 × 10 3 PFU mL −1 and 4 × 10 4 PFU mL −1 to be achieved for the H1N1 and H3N2 strains respectively. In contrast, a classical ELISA test based on the same antibody sandwich showed detection limit of 1.2 × 10 7 PFU mL −1 for H1N1. The new enhanced MELISA proved to be specific, as no cross-reactivity was found with a porcine respiratory virus (PRRSV).

show abstract

Femtomolar Detection of Dengue Virus DNA with Serotype Identification Ability

Cited by 59 publications

References 38 publications

Human virus detection with graphene-based materials

Human virus detection with graphene-based materials

DNA/RNA Electrochemical Biosensing Devices a Future Replacement of PCR Methods for a Fast Epidemic Containment

Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay

Contact Info

Product

Resources

About