Female Specific Protein: Biosynthesis Controlled by Corpus Allatum in
            <i>Leucophaea maderae</i>

Engelmann, Franz

doi:10.1126/science.165.3891.407

Cited by 112 publications

(21 citation statements)

References 6 publications

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“…Serum proteins specific for one sex have been described in many species. Numerous examples exist in insects (1) where hormonally controlled female specific proteins have been detected in serums during egg production and are necessary for egg maturation (2). Similarly, a phosphoprotein, called "serum vitellin" by Laskowski (3) was found in the plasma of laying hens.…”

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confidence: 99%

A sex-limited serum protein of Syrian hamsters: definition of female protein and regulation by testosterone.

Coe¹

1977

Proc. Natl. Acad. Sci. U.S.A.

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Normal Syrian hamster females contain a serum protein not found by simple gel diffusion assay in normal adult males. This sex-limited protein was called female protein (FP). Low levels of FP were found in sera from normal weanling male hamsters. Adult male hamsters castrated or treated with diethylstilbestrol also developed serum FP, which could be suppressed by administration of testosterone. Exogenous testosterone also suppressed serum FP in adult females, and ovarian function did not appear to be critical for maintenance of serum FP. Although FP is present in significant amounts (1-2 mg/ml) in adult females, its function is presently unknown. Serum proteins specific for one sex have been described in many species. Numerous examples exist in insects (1) where hormonally controlled female specific proteins have been detected in serums during egg production and are necessary for egg maturation (2). Similarly, a phosphoprotein, called "serum vitellin" by Laskowski (3) was found in the plasma of laying hens. This protein was also found in cockerels after diethylstilbestrol (DES) treatment (4) and along with a lipoprotein component (5) apparently acts as a transport system for the developing yolk. Another sex-linked serum protein called "hormone-influenced protein" has been detected in some normal laying hens and in other chickens (male and female) after progesterone treatment (6).In mammals, sex-differences in serum levels of IgM (7, 8) have been described and two mouse complement components, C5 and C6, have been shown to be under sex hormone control (9,10 Freund's adjuvant. Specific antisera to FP were obtained either by direct addition of absorbing antigen (normal male hamster serum, NMHS) to the antisera and removal of precipitins by centrifugation or usually by passage of the antiserum through a column of Sepharose 2B (Pharmacia, Uppsala, Sweden) to which NMHS had been coupled by cyanogen bromide (14). FP was isolated by passing normal female hamster sera (NFHS) through a column containing Sepharose 2B conjugated by cyanogen bromide to the one-half saturated ammonium sulfate precipitate of specific rabbit antisera against FP. The column was thoroughly washed with phosphate buffered saline at pH 7.2 and the FP was eluted with 0.5 M acetic acid, promptly neutralized with Tris base, and then concentrated by negative pressure and dialyzed against phosphate-buffered saline.Immunodiffusion Studies. Agar electrophoresis and immunoelectrophoresis were performed on 2% agar covered microscope slides using 7 V/cm with barbital buffer at pH 8.2 (ionic strength 0.04). Agar electrophoresis patterns of FP were stained with various dyes according to Uriel (15). Simple gel diffusion was done on 1% agarose covered slides. FP was quantified by radial diffusion on glass slides covered with 1% agar containing specific rabbit antisera against FP. Five microliter samples were placed into 14 gauge needle holes and after 24 hr at room temperature the precipitin diameters were measured. The squared diameters were then compared...

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confidence: 99%

A sex-limited serum protein of Syrian hamsters: definition of female protein and regulation by testosterone.

Coe¹

1977

Proc. Natl. Acad. Sci. U.S.A.

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“…The female-specific, vitellogenic haemolymph protein, vitellogenin, is synthesized in the fat body under the influence of the juvenile hormone (ENGELMANN, 1969;BROOKES, 1969;PAN et al, 1969;HAGEDORN and JUDSON, 1972), released into the haemolymph, and enters the oocyte by pinocytosis (TELFER, 1961 ;ROTH and PORTER, 1964;ANDERSON and SPIELMAN, 1971). The vitellogenin becomes 20-30 times more concentrated in the oocyte than in the haemolymph while other proteins are largely excluded (TELFER, 1960).…”

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confidence: 99%

Vitellogenin in the Eggs of the Cockroach, Blattella Germanica: Purification and Characterization

1975

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Vitellogenin in the eggs of Blattella germanica was solubilized with solutions at high salt concentrations and high pH. This protein was purified by ammonium sulfate precipitation, acetic acid precipitation, DEAE‐cellulose chromatography, and hydroxylapatite chromatography, into a chromatographically homogeneous state. By sucrose density gradient centrifugation, the purified vitellogenin was resolved into two components. The relative amounts of the two components varied according to the pH of the solution. An equilibrium seemed to exist in the interconversion between them when the conditions of the solution were fixed. It is suggested that aggregation and disaggregation of the vitellogenin molecules may account for the apparent heterogeneity.

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“…The site of synthesis of vitellogenin has been proved to be the fat body (BROOKS, 1969;ENGELMANN, 1969, PAN et al, 1969HAGEDORN and JUDSON, 1972) and the extract of fat body has been shown to contain vitellogenin in Musca domesticu (BODNARYK and MORRISON, 1968) and Leucophaea maderae (ENGELMANN, 1969). The present study is the first to demonstrate immunohistochemically the presence of vitellogenin in the fat body.…”

Section: Discussionmentioning

confidence: 66%

“…We now know that it is synthesized by the fat body (BROOKES, 1969;ENGELMANN, 1969;PAN et al, 1969;HAGEDORN and JUDSON, 1972), released into the blood, and incorporated into the yolk protein bodies in preference to other circulating proteins (TELFER, 1954;.…”

mentioning

confidence: 99%

IMMUNOHISTOCHEMICAL DETECTION OF VITELLOGENIN IN THE OVARY AND FAT BODY DURING A REPRODUCTIVE CYCLE OF THE COCKROACH, BLATTELLA GERMANICA

Ishizaki

1974

Dev Growth Differ

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was immunohistochemically demonstrated in .the ovary and fat body during a reproductive cycle. In terminal oocytes of 4-day adults, vitdogenin began t o appear in yolk spherules at the cell periphery. The vitellogenin-containing spiierules increased in size and number t o occupy the whole cell 2 days later. In the female fat body, vitellogenin began to appear in 3-day adults. It was distributed diffusely in the cytoplasm, at a much lower concentration than in the oocytes, during the vitellogenic period. In 1 I-day adults whose vitellogenesis had terminated, a higher concentration of vitellogenin was found in the cytoplasmic inclusions of the fat body. Vitellogenin was not detected in the male fat body.

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Female Specific Protein: Biosynthesis Controlled by Corpus Allatum in Leucophaea maderae

Cited by 112 publications

References 6 publications

A sex-limited serum protein of Syrian hamsters: definition of female protein and regulation by testosterone.

A sex-limited serum protein of Syrian hamsters: definition of female protein and regulation by testosterone.

Vitellogenin in the Eggs of the Cockroach, Blattella Germanica: Purification and Characterization

IMMUNOHISTOCHEMICAL DETECTION OF VITELLOGENIN IN THE OVARY AND FAT BODY DURING A REPRODUCTIVE CYCLE OF THE COCKROACH, BLATTELLA GERMANICA

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