Female-specific doublesex dsRNA interrupts yolk protein gene expression and reproductive ability in oriental fruit fly, Bactrocera dorsalis (Hendel)

Chen, Shiu-Ling; Dai, Shu‐Mei; Lu, Kuang‐Hui; Chang, Cheng‐Nan

doi:10.1016/j.ibmb.2007.10.003

Cited by 79 publications

(76 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Duox has been extensively studied for its role in the antibacterial defense against infectious bacteria; our study highlights the importance of BdDuox in the control of intestinal bacterial community homeostasis in the intestine. The silencing effect of target genes by RNAi techniques lasts for only 4-6 days in B. dorsalis, as previously reported (Chen et al, 2008;Li et al, 2015), and the expression level of the BdDuox gene was downregulated with the 1-7 DPR treatment. The silencing of the BdDuox gene led to an increase in intestinal bacterial density and altered both the structure and richness of the intestinal indigenous bacterial community.…”

Section: Discussionsupporting

confidence: 72%

The dual oxidase gene BdDuox regulates the intestinal bacterial community homeostasis of Bactrocera dorsalis

Yao¹,

Wang²,

Li³

et al. 2015

The ISME Journal

View full text Add to dashboard Cite

The guts of metazoans are in permanent contact with the microbial realm that includes beneficial symbionts, nonsymbionts, food-borne microbes and life-threatening pathogens. However, little is known concerning how host immunity affects gut bacterial community. Here, we analyze the role of a dual oxidase gene (BdDuox) in regulating the intestinal bacterial community homeostasis of the oriental fruit fly Bactrocera dorsalis. The results showed that knockdown of BdDuox led to an increased bacterial load, and to a decrease in the relative abundance of Enterobacteriaceae and Leuconostocaceae bacterial symbionts in the gut. The resulting dysbiosis, in turn, stimulates an immune response by activating BdDuox and promoting reactive oxygen species (ROS) production that regulates the composition and structure of the gut bacterial community to normal status by repressing the overgrowth of minor pathobionts. Our results suggest that BdDuox plays a pivotal role in regulating the homeostasis of the gut bacterial community in B. dorsalis.

show abstract

Section: Discussionsupporting

confidence: 72%

The dual oxidase gene BdDuox regulates the intestinal bacterial community homeostasis of Bactrocera dorsalis

Yao¹,

Wang²,

Li³

et al. 2015

The ISME Journal

View full text Add to dashboard Cite

show abstract

“…In the tephritids, the gene tra controls also the sex-specific splicing of the dsx primary transcript (Pane et al, 2002;Lagos et al, 2007;Salvemini et al, 2009). The gene dsx has been characterised in the tephritids Bactrocera tryoni (Shearman and Frommer 1998), Bactrocera oleae (Lagos et al, 2005), Bactrocera dorsalis (Chen et al, 2008), Ceratitis capitata , and in eleven Anastrepha species 2007b). The molecular organisation of the dsx ORF varies among these insects, but in all cases dsx encodes male-and female-specific RNAs that encode putative male-and femalespecific Dsx proteins that share the N-terminal region but which differ in their C-terminal regions (as in Drosophila).…”

Section: B C D E Fmentioning

confidence: 99%

Effect of the gene transformer of Anastrepha on the somatic sexual development of Drosophila

Ruiz¹,

Sánchez

2010

Int. J. Dev. Biol.

View full text Add to dashboard Cite

The gene transformer (tra) is the key regulatory memory device for sex determination in tephritid insects. The present manuscript addressed the question about the functional conservation of the tephritid Anastrepha Transformer protein to direct somatic sexual development in Drosophila (Drosophilidae). The transformer cDNA of Anastrepha encoding the putative fulllength Tra protein was cloned in pUAST and introduced into Drosophila melanogaster. To express this protein, the GAL4-UAS system was used. The Anastrepha Tra protein induced the femalespecific splicing of both dsx and fru pre-mRNAs in Drosophila XY male flies, so that these became transformed into females, though this transformation was incomplete (the sexually dimorphic foreleg basitarsus and the external terminalia were monitored). It was found that the degree of female transformation directly depended on the dose of Anastrepha tra and Drosophila transformer-2 (tra-2) genes, and that the Anastrepha Tra-Drosophila Tra2 complex is not as efficient as the Drosophila Tra-Tra2 complex at inducing the female-specific splicing of Drosophila dsx premRNA. This can explain why the Anastrepha Tra protein cannot fully substitute for the endogenous Drosophila Tra protein.

show abstract

“…As the blockage of SODD expression in leukemic cells would be conducive to the restoration of the sensitivity of tumor cells during chemotherapy and to the increase in survival rate, SODD-targeting gene therapy can be of great potential value (Chen et al, 2008Ito et al, 2011). Gene therapy is a technique for correcting defective genes or abnormal gene expression responsible for disease development.…”

Section: Discussionmentioning

confidence: 99%

Significance of SODD expression in childhood acute lymphoblastic leukemia and its influence on chemotherapy

Tao¹,

Liu²,

Fang³

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. This study explored the clinical significance of silencer of death domain (SODD) expression in childhood acute lymphoblastic leukemia (ALL) and its influence on chemotherapy as well as the effect of SODD expression on apoptosis of leukemic cells. The expression of SODD proteins in different ALL groups was determined by immunocytochemistry. The SODD RNAi-interfering plasmid was constructed and transferred to Jurkat cells, and the effects of SODD expression on cell proliferation and apoptosis were analyzed using the MTT and FCM methods. The expressions of SODD, Phospho-NF-κB-P65, Bcl-2, and Caspase 3 were detected by Western blot analysis. The expression of SODD proteins was significantly higher in the ALL groups than in the control group (P < 0.05). The positive expression rate of SODD was significantly higher in refractory/relapsed and clinical high-risk groups than in standard-risk, initial treatment, and complete remission groups (P < 0.05). Microtubule-targeting drugs such as vincristine and taxol can notably down-regulate SODD expression during apoptosis, whereas DNR, and Ara-c cannot. The sensitivity of Jurkat cells to chemotherapeutic drugs increased with down-regulated SODD expression induced by SODD-interfering plasmid transfection. The sensitivity of the cells transfected with SODD-cloning genes decreased. SODD expression was high in the ALL children. These findings indicated that SODD over-expression might be correlated with the clinical classification, curative effect, and prognosis of ALL cells. Microtubule-targeting drugs can specifically down-regulate SODD expression in leukemic cells, thereby increasing the sensitivity of leukemic cells to SODD-targeting chemotherapeutics. In contrast, increased SODD expression tends to reduce sensitivity.

show abstract

Female-specific doublesex dsRNA interrupts yolk protein gene expression and reproductive ability in oriental fruit fly, Bactrocera dorsalis (Hendel)

Cited by 79 publications

References 46 publications

The dual oxidase gene BdDuox regulates the intestinal bacterial community homeostasis of Bactrocera dorsalis

The dual oxidase gene BdDuox regulates the intestinal bacterial community homeostasis of Bactrocera dorsalis

Effect of the gene transformer of Anastrepha on the somatic sexual development of Drosophila

Significance of SODD expression in childhood acute lymphoblastic leukemia and its influence on chemotherapy

Contact Info

Product

Resources

About