Female sex pheromone of <i>Athetis lepigone</i> (Lepidoptera: Noctuidae): Identification and field evaluation

Yan, Qi; Zheng, Mei-Yan; Xu, Junwei; Ma, Ji-Fang; Chen, Yu; Dong, Zimei; Liu, L.; Dong, Shuang-Lin; Zhang, Y.-N.

doi:10.1111/jen.12413

Cited by 18 publications

(21 citation statements)

References 21 publications

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“…Subsequently, we analyzed the relative binding affinities of purified AlepOBP6 protein to 20 different ligands using 1‐NPN as a fluorescent probe, including two sex pheromones ( Z 7‐12:Ac and Z 9‐14:Ac) from female A. lepigone 32 and 18 host plant (maize) volatiles 46–49 (Figure 2B–D and Table 1). The results showed that AlepOBP6 could bind to the sex pheromones with K i values of 21.21 ± 2.12 μ m ( Z 7‐12:Ac) and 14.50 ± 1.44 μ m ( Z 9‐14:Ac).…”

Section: Resultsmentioning

confidence: 99%

“…One of these novel approaches is based on understanding the mechanisms of insect olfaction that may lead to strategies disrupting host plant detection and other olfactory behaviors, thereby allowing insect populations to be controlled. We previously identified two female sex pheromones of A. lepigone , ( Z )‐7‐dodecenyl acetate ( Z 7‐12:Ac) and ( Z )‐9‐tetradecadienyl acetate ( Z 9‐14:Ac), 32 and 28 OBP genes, including ABPX (AlepOBP6), which showed male antennae‐biased expression, indicating that AlepOBP6 may be involved in some behaviors associated with males, such as recognition of female sex pheromones and host volatiles 33 . In this study, we further investigated the olfactory function of AlepOBP6 in A. lepigone , and binding affinities to two sex pheromones and 18 maize volatiles were examined using a fluorescence competitive binding approach.…”

Section: Introductionmentioning

confidence: 99%

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Ligand‐binding properties of odorant‐binding protein 6 in Athetis lepigone to sex pheromones and maize volatiles

Huang

et al. 2021

Pest Management Science

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BACKGROUND Athetis lepigone, a noctuid moth feeding on more than 30 different crops worldwide, has evolved a sophisticated, sensitive, and specific chemosensory system to detect and discriminate exogenous chemicals. Odorant‐binding proteins (OBPs) are the most important agent in insect chemosensory systems to be explored as an alternative target for environmentally friendly approaches to pest management. RESULTS To investigate the olfactory function of A. lepigone OBPs (AlepOBPs), AlepOBP6 was identified and expressed in Escherichia coli. The binding affinity of the recombinant OBP to 20 different ligands was then examined using a competitive binding approach. The results revealed that AlepOBP6 can bind to two sex pheromones and ten maize volatiles, and its conformation stability is pH dependent. We also carried out a structure–function study using different molecular approaches, including structure modeling, molecular docking, and a mutation functional assay to identify amino acid residues (M39, V68, W106, Q107, and Y114) involved in the binding of AlepOBP6 to both sex pheromones and maize volatiles in A. lepigone. CONCLUSION These results suggest that AlepOBP6 is likely involved in mediating the responses of A. lepigone to sex pheromones and maize volatiles, which may play a pivotal function in mating, feeding, and oviposition behaviors. This study not only provides new insight into the binding mechanism of OBPs to sex pheromones and host volatiles in moths, but also contributes to the discovery of novel target candidates for developing efficient behavior disruptors to control A. lepigone in the future. © 2021 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ligand‐binding properties of odorant‐binding protein 6 in Athetis lepigone to sex pheromones and maize volatiles

Huang

et al. 2021

Pest Management Science

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“…As a newly invasive pest in China, S. frugiperda shares pheromone components with some local moth species. Athetis lepigone and Mythimna loreyi share the same pheromone components, Z 9‐14:Ac and Z 7‐12:Ac with S. frugiperda and have similar component ratios (Takahashi et al ., 1980; Yan et al ., 2018). To avoid cross‐specific attraction and improve monitoring accuracy, the pheromone synergists and antagonists of these species need to be further developed.…”

Section: Discussionmentioning

confidence: 99%

Revisiting the sex pheromone of the fall armyworm Spodoptera frugiperda, a new invasive pest in South China

Jiang

Guo

et al. 2021

Insect Science

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The fall armyworm Spodoptera frugiperda is a worldwide serious agricultural pest, and recently invaded South China. Sex pheromone can be employed to monitor its population dynamics accurately in the field. However, the pheromone components previously reported by testing different geographic populations and strains are not consistent. On the basis of confirming that the S. frugiperda population from Yunnan Province belonged to the corn strain, we analyzed the potential sex pheromone components in the pheromone gland extracts of females using gas chromatography coupled with electroantennographic detection (GC‐EAD), gas chromatography coupled with mass spectrometry (GC‐MS) and electroantennography (EAG). The results show that (Z)‐9‐tetradecenal acetate (Z9‐14:Ac), (Z)‐11‐hexadecenyl acetate (Z11‐16:Ac), (Z)‐7‐dodecenyl acetate (Z7‐12:Ac) or (E)‐7‐dodecenyl acetate (E7‐12:Ac) with a ratio of 100 : 15.8 : 3.9 induced EAD responses to varying degrees: Z9‐14:Ac elicited a strong EAD response, Z7‐12:Ac or E7‐12:Ac elicited a small but clear EAD response, while Z11‐16:Ac elicited a weak EAD response. Further single sensillum recording (SSR) showed that Z9‐14:Ac and Z7‐12:Ac induced dose‐dependent activities in two types (A and B) of sensilla in male antennae, respectively, while the sensilla in response to E7‐12:Ac and Z11‐16:Ac was not recorded. Finally, wind tunnel tests reveal that Z9‐14:Ac and Z7‐12:Ac are two principal sex pheromone components of the tested population.

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“…The development of novel green behavioral inhibitors based on olfactory genes has become a hot spot research field for pest control (Pelosi et al ., 2018; Caballero-Vidal et al ., 2021). In order to fully understand the concept of insect chemical communication, we have recently identified two female sex pheromone of A. lepigone as (Z)-7-dodecenyl acetate (Z7-12:Ac) and (Z)-9-tetradecenyl acetate (Z9-14:Ac) with a ratio of 1:5 by analyzing the extracts of the female sex pheromone gland (Yan et al ., 2018), and obtained three antennae-enriched PBP genes (AlepPBP1-3) in A. lepigone by antennal transcriptome analysis (Zhang et al ., 2017). Afterward, we expressed and purified the recombinant AlepPBP1 protein in a prokaryotic expression system.…”

Section: Introductionmentioning

confidence: 99%

AlepPBP2, but not AlepPBP3, may involve in the recognition of sex pheromones and maize volatiles inAthetis lepigone

Yang

Zhang

et al. 2022

Bull. Entomol. Res.

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Athetis lepigone Möschler (Lepidoptera, Noctuidae) is a common maize pest in Europe and Asia. However, there is no long-term effective management strategy is available yet to suppress its population. Adults rely heavily on olfactory cues to locate their optimal host plants and oviposition sites. Pheromone-binding proteins (PBPs) are believed to be responsible for recognizing and transporting different odorant molecules to interact with receptor membrane proteins. In this study, the ligand-binding specificities of two AlepPBPs (AlepPBP2 and AlepPBP3) for sex pheromone components and host plant (maize) volatiles were measured by fluorescence ligand-binding assay. The results demonstrated that AlepPBP2 had a high affinity with two pheromones [(Z)-7-dodecenyl acetate, Ki = 1.11 ± 0.1 μM, (Z)-9-tetradecenyl acetate, Ki = 1.32 ± 0.15 μM] and ten plant volatiles, including (-)-limonene, α-pinene, myrcene, linalool, benzaldehyde, nonanal, 2-hexanone, 3-hexanone, 2-heptanone and 6-methyl-5-hepten-2-one. In contrast, we found that none of these chemicals could bind to AlepPBP3. Our results clearly show no significant differences in the functional characterization of the binding properties between AlepPBP2 and AlepPBP3 to sex pheromones and host plant volatiles. Furthermore, molecular docking was employed for further detail on some crucial amino acid residues involved in the ligand-binding of AlepPBP2. These findings will provide valuable information about the potential protein binding sites necessary for protein-ligand interactions which appear as attractive targets for the development of novel technologies and management strategies for insect pests.

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Female sex pheromone of Athetis lepigone (Lepidoptera: Noctuidae): Identification and field evaluation

Cited by 18 publications

References 21 publications

Ligand‐binding properties of odorant‐binding protein 6 in Athetis lepigone to sex pheromones and maize volatiles

Ligand‐binding properties of odorant‐binding protein 6 in Athetis lepigone to sex pheromones and maize volatiles

Revisiting the sex pheromone of the fall armyworm Spodoptera frugiperda, a new invasive pest in South China

AlepPBP2, but not AlepPBP3, may involve in the recognition of sex pheromones and maize volatiles inAthetis lepigone

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