Female Sex Development and Reproductive Duct Formation Depend on Wnt4a in Zebrafish

Kossack, Michelle E.; High, Samantha K.; Hopton, Rachel E.; Yan, Yi‐Lin; Postlethwait, John H.; Draper, Bruce W.

doi:10.1534/genetics.118.301620

Cited by 46 publications

(43 citation statements)

References 54 publications

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“…Therefore, CPE is still a good candidate for fish artificial propagation since it is rich in hormones and some factors, while the functions of some hormones have not been identified so far. Wnt4a and the miR-200 cluster on chromosome 23 are required for female reproductive duct formation in zebrafish and loss of each function led to defective reproductive duct development (Kossack et al, 2019;Xiong et al, 2020), which is similar to the phenotype of defective yellow catfish in current study. Interestingly, Oxytocin and Vasotocin are potentially responsible for zebrafish ovulation (Xiong et al, 2020).…”

Section: Discussionsupporting

confidence: 84%

Synergistic Combination of Exogenous Hormones to Improve the Spawning and Post-spawning Survival of Female Yellow Catfish

Huang

Xiong

et al. 2020

Front. Genet.

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Multiple repeat spawners make large contributions to long-term population stability and aquaculture breeding programs. A high percentage of female yellow catfish (Pelteobagrus fulvidraco) died for spawning failure or incomplete spawning after artificial spawning by traditional synthetic hormones including human chorionic gonadotropin (hCG), luteinizing hormone releasing hormone (LHRH), and domperidone (DOM). The present study was designed to compare the efficacy of different combinations of exogenous hormones for inducing ovulation in yellow catfish using hCG, LHRH, DOM, and carp pituitary extraction (CPE). We found a optimal strategy for exogenous hormones administration, the mixture of LHRH/CPE for the first injection and LHRH/CPE/DOM for the second injection, could greatly improve the rates of spawning success, weight of ovulated eggs and survival rate after spawning. Interestingly, a population of female yellow catfish with defective reproductive duct could not spawn and showed high mortality after induced by a combination of hCG/LHRH/DOM, whereas a synergistic combination of hCG, LHRH, DOM, and CPE could efficiently induce spawning and reduce mortality in the defective yellow catfish, in which a significant decrease of Vitellin and E2 levels. Altogether, our findings provide an effective combination of exogenous hormones to improve spawning and post-spawning survival of female yellow catfish.

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Section: Discussionsupporting

confidence: 84%

Synergistic Combination of Exogenous Hormones to Improve the Spawning and Post-spawning Survival of Female Yellow Catfish

Huang

Xiong

et al. 2020

Front. Genet.

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“…Additionally, the D. rerio ortholog of the C. harengus trophoblast glycoprotein like ( tpbgl ) gene is wnt-activated inhibitory factor 2 ( waif2), which has been shown to be a modifier of Wnt signalling pathways ( Table 5 ). Canonical wnt pathways are important for both mammalian and D. rerio sex determination ( Harris et al, 2018 ; Jordan et al, 2001 ; Kossack et al, 2019 ; Sreenivasan et al, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

“…differentiation processes as it is involved in oocyte maturation in D. rerio and plays a role in sexual maturation in male sea lamprey (Petromyzon marinus). Additionally, the D. rerio ortholog of the C. harengus trophoblast glycoprotein like (tpbgl) gene is wntactivated inhibitory factor 2 (waif2), which has been shown to be a modifier of Wnt signalling pathways ( (Harris et al, 2018;Jordan et al, 2001;Kossack et al, 2019;Sreenivasan et al, 2014).…”

Section: Fishmentioning

confidence: 99%

Identification of male heterogametic sex‐determining regions on the Atlantic herring Clupea harengus genome

Kongsstovu

Dahl²,

Gislason

et al. 2020

Journal of Fish Biology

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The sex determination system of Atlantic herring Clupea harengus L., a commercially important fish, was investigated. Low coverage whole-genome sequencing of 48 females and 55 males and a genome-wide association study revealed two regions on chromosomes 8 and 21 associated with sex. The genotyping data of the single nucleotide polymorphisms associated with sex showed that 99.4% of the available female genotypes were homozygous, whereas 68.6% of the available male genotypes were heterozygous. This is close to the theoretical expectation of homo/heterozygous distribution at low sequencing coverage when the males are factually heterozygous. This suggested a male heterogametic sex determination system in C. harengus, consistent with other species within the Clupeiformes group. There were 76 protein coding genes on the sex regions but none of these genes were previously reported master sex regulation genes, or obviously related to sex determination. However, many of these genes are expressed in testis or ovary in other species, but the exact genes controlling sex determination in C. harengus could not be identified.

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“…The zebrafish genome contains two Wnt4 orthologs, called wnt4a and wnt4b (Liu, Majumdar, Schauerte, Haffter, & Drummond, 2000; Ungar, Kelly, & Moon, 1995). Though many of the gene duplicates that are present in zebrafish resulted from the teleost-specific whole genome duplication that occurred after this lineage diverged from that of tetrapods, phylogenetic analysis performed by Kossack et al (2019) argues instead that the duplication event that resulted in wnt4a and wnt4b occurred prior to the divergence of teleost and tetrapods. First, while tetrapod genomes contain a single Wnt4 gene, two Wnt4 genes are present in more basal tetrapods, such as turtles and Coelacanths.…”

Section: Female-promoting Genesmentioning

confidence: 99%

“…Thus, the expression pattern of wnt4a in zebrafish is similar to the expression of Wnt4 in mammals (Vainio et al, 1999). Kossack et al (2019) tested the role of Wnt4a in sex determination using wnt4a point mutants and CRISPR/Cas9-induced indel mutants. They found that while wild-type siblings had normal ratios of males and females (near 50:50), ~95% of wnt4a mutant animals developed as males.…”

Section: Female-promoting Genesmentioning

confidence: 99%

Genetic regulation of sex determination and maintenance in zebrafish (Danio rerio)

Kossack

Draper

2019

Current Topics in Developmental Biology

113

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Over the last several decades zebrafish (Danio rerio) has become a major model organism for the study of vertebrate development and physiology. Given this, it may be surprising how little is known about the mechanism that zebrafish use to determine sex. While zebrafish are a gonochoristic species (having two sexes) that do not switch sex as adults, it was appreciated early on that sex ratios obtained from breeding lab domesticated lines were not typically a 1:1 ratio of male and female, suggesting that sex was not determined by a strict chromosomal mechanism. Here we will review the recent progress toward defining the genetic mechanism for sex determination in both wild and domesticated zebrafish.

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Female Sex Development and Reproductive Duct Formation Depend on Wnt4a in Zebrafish

Abstract: Wnt4 is a key regulator of ovary development in mammals, but its role in other vertebrates is unknown. Here, Kossack et al. show that zebrafish wnt4a is the ortholog of mammalian Wnt4 and is expressed....

Cited by 46 publications

References 54 publications

Synergistic Combination of Exogenous Hormones to Improve the Spawning and Post-spawning Survival of Female Yellow Catfish

Synergistic Combination of Exogenous Hormones to Improve the Spawning and Post-spawning Survival of Female Yellow Catfish

Identification of male heterogametic sex‐determining regions on the Atlantic herring Clupea harengus genome

Genetic regulation of sex determination and maintenance in zebrafish (Danio rerio)

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