Female sex and Western-style diet protect mouse resistance arteries during acute oxidative stress

Norton, Charles E.; Jacobsen, Nicole L.; Sinkler, Shenghua Y.; Manrique‐Acevedo, Camila; Segal, Steven S.

doi:10.1152/ajpcell.00342.2019

Cited by 16 publications

(51 citation statements)

References 56 publications

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“…MDA is the end product of lipid peroxidation and is an essential biochemical parameter used as an oxidative stress marker. Both the ketogenic diet and the western diet were reported to affect oxidative stress (Norton et al, 2020). In the present study, it was observed that feeding with short-term KD caused increases in antioxidant parameters in the liver.…”

Section: Discussionsupporting

confidence: 54%

“…Although the physiological mechanisms of these diets on organs are not well understood, both experimental and human studies have shown that obesity is associated with increased oxidative stress markers and lipid peroxidation (Wilson et al, 2007). In the studies examining the effects of feeding with WD; unlike KD, WD has been reported to increase oxidative stress (Jenkins et al, 2016;Norton et al, 2020). Irregularity of hepatic lipogenesis and prolonged exposure of the heart to a high fuel supply have been shown to be important causes of heart failure in rats fed with high-fat WD (Harmancey et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Irregularity of hepatic lipogenesis and prolonged exposure of the heart to a high fuel supply have been shown to be important causes of heart failure in rats fed with high-fat WD (Harmancey et al, 2010). In a different study, it was stated that even though WD increases oxidative stress, vascular cells adapt to this stress by resisting (Norton et al, 2020). In rats fed a diet like a WD diet which is rich in saturated fatty acids and fructose, oxidative stress markers (lipid peroxidation and Nitro-Tyrosine content) were significantly increased, but the antioxidant enzyme CAT decreased (Mazzoli et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

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Effects of ketogenic and western diets on proliferation, vasculogenesis and oxidative stress in the liver

Doğanay¹,

Budak²,

Bahtiyar³

et al. 2021

JECM

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In this study, it was aimed to investigate the effects of different diets on lipid peroxidation, antioxidants, proliferation and vasculogenesis in liver tissue. BALBC female rats (21) were divided as the group fed with standard chow (SC), the group fed with a ketogenic diet (KD) and the group fed with western diet (WD). The rats were fed with tap water and a rat diet specially prepared according to the determined diets for 4 weeks. Liver tissue oxidative damage, proliferation and vasculogenesis were evaluated using spectrophotometric and immunohistopathological methods. At the end of the experiment, it was found that the highest weight gain was in the WD group and the least weight gain was in the KD group. The mean body weight of the WD group was statistically significantly higher compared to the SC and KD groups (p<0.05). MDA levels were found to be lower in the KD group compared to the SC and WD groups. GSH and CAT levels were higher in the KD group compared to the SC and WD groups. In IHC evaluation results, it was seen that Ki-67 percentage in the KD group increased compared to the WD and SC groups (p = 0.000), VEGF was close to each other in all three groups and there was no significant difference in the comparisons between groups (p>0.05). These results revealed that ketogenic diet prevents tissue damage by decreasing lipid peroxidation in liver tissue as well as inducing cellular proliferation and vasculogenesis.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Effects of ketogenic and western diets on proliferation, vasculogenesis and oxidative stress in the liver

Doğanay¹,

Budak²,

Bahtiyar³

et al. 2021

JECM

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“…Although we did not detect changes in Nos3 mRNA, it is not known whether NO production or bioavailability was altered. Interestingly, greater resistance to oxidant stress has been reported in endothelial cells of rodents pre‐conditioned with HF diet, which may protect against endothelial dysfunction. It also was reported that ischaemia itself blunts vasodilatory responses within the microvascular networks of post‐ischaemic muscle .…”

Section: Discussionmentioning

confidence: 99%

High‐fat diet pre‐conditioning improves microvascular remodelling during regeneration of ischaemic mouse skeletal muscle

et al. 2020

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Aim Critical limb ischaemia (CLI) is characterized by inadequate angiogenesis, arteriolar remodelling and chronic myopathy, which are most severe in type 2 diabetic patients. Hypertriglyceridaemia, commonly observed in these patients, compromises macrovascular function. However, the effects of high‐fat diet‐induced increases in circulating lipids on microvascular remodelling are not established. Here, we investigated if high‐fat diet would mimic the detrimental effect of type 2 diabetes on post‐ischaemia vascular remodelling and muscle regeneration, using a mouse model of hindlimb ischaemia. Methods Male C57Bl6/J mice were fed with normal or high‐fat diets for 8 weeks prior to unilateral femoral artery ligation. Laser doppler imaging was used to assess limb perfusion recovery. Vascular recovery, inflammation, myofibre regeneration and fibrosis were assessed at 4 or 14 days post‐ligation by histology and RNA analyses. Capillary‐level haemodynamics were assessed by intravital microscopy of control and regenerating muscles 14 days post‐ligation. Results High‐fat diet increased muscle succinate dehydrogenase activity and capillary‐level oxygen supply. At 4 days post‐ligation, no diet differences were detected in muscle damage, inflammatory infiltration or capillary activation. At 14 days post‐ligation, high fat‐fed mice displayed accelerated limb blood flow recovery, elevated capillary and arteriole densities as well as greater red blood cell supply rates and capillary‐level oxygen supply. Regenerating muscles from high fat‐fed mice displayed lower interstitial fat and collagen deposition. Conclusion The muscle‐level adaptations to high‐fat diet improved multiple aspects of muscle recovery in response to ischaemia and did not recapitulate the worse outcomes seen in diabetic CLI patients.

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“…The copyright holder for this preprint this version posted August 4, 2021. ; https://doi.org/10.1101/2021.08.02.454805 doi: bioRxiv preprint 28 dying cells (14,15). Following BaCl2 exposure, respective nuclear dyes (in standard PSS) were perfused through the lumen of a cannulated SEA (0.1 mL/min) or superfused over the surface of an endothelial tube for min followed by min wash in standard PSS.…”

Section: Cell Deathmentioning

confidence: 99%

Myofiber injury induces capillary disruption and regeneration of disorganized microvascular networks

Jacobsen

Norton

Shaw

et al. 2021

Preprint

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Myofibers regenerate following injury, however the microvasculature must also recover to restore skeletal muscle function. We aimed to define the nature of microvascular damage and repair during skeletal muscle injury and regeneration induced by BaCl2. To test the hypothesis that microvascular disruption occurred secondary to myofiber injury in mice, isolated microvessels were exposed to BaCl2 or the myotoxin was injected into the gluteus maximus (GM) muscle. In isolated microvessels, BaCl2 depolarized smooth muscle cells and endothelial cells while increasing [Ca2+]i, but did not elicit cell death. At 1 day post injury (dpi) of the GM, capillary fragmentation coincided with myofiber degeneration while arteriolar and venular networks remained intact; neutrophil depletion before injury did not prevent capillary damage. Perfused capillary networks reformed by 5 dpi in association with more terminal arterioles and were dilated through 10 dpi; with no change in microvascular area or branch point number in regenerating networks, fewer capillaries aligned with myofibers and capillary networks were no longer organized into microvascular units. By 21 dpi, capillary orientation and organization had nearly recovered to that in uninjured GM. We conclude that following their disruption secondary to myofiber damage, capillaries regenerate as disorganized networks that remodel while regenerated myofibers mature.

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Female sex and Western-style diet protect mouse resistance arteries during acute oxidative stress

Cited by 16 publications

References 56 publications

Effects of ketogenic and western diets on proliferation, vasculogenesis and oxidative stress in the liver

Effects of ketogenic and western diets on proliferation, vasculogenesis and oxidative stress in the liver

High‐fat diet pre‐conditioning improves microvascular remodelling during regeneration of ischaemic mouse skeletal muscle

Myofiber injury induces capillary disruption and regeneration of disorganized microvascular networks

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