Female Gametophyte Development

Yadegari, Ramin; Drews, Gary N.

doi:10.1105/tpc.018192

Cited by 368 publications

(318 citation statements)

References 86 publications

Supporting

Mentioning

314

Contrasting

Unclassified

Order By: Relevance

“…The female gametophyte is embedded in sporophytic tissues and its development depends on them (Yang and Sundaresan, 2000;Yadegari and Drews, 2004). Missing or abnormal integuments result in meiotic arrest, which is supported by observations on the aintegumenta and bel1 mutants (Reiser et al, 1995;Klucher et al, 1996).…”

Section: Introductionmentioning

confidence: 57%

See 1 more Smart Citation

Truncation of a Protein Disulfide Isomerase, PDIL2-1, Delays Embryo Sac Maturation and Disrupts Pollen Tube Guidance inArabidopsis thaliana

et al. 2008

View full text Add to dashboard Cite

Pollen tubes must navigate through different female tissues to deliver sperm to the embryo sac for fertilization. Protein disulfide isomerases play important roles in the maturation of secreted or plasma membrane proteins. Here, we show that certain T-DNA insertions in Arabidopsis thaliana PDIL2-1, a protein disulfide isomerase (PDI), have reduced seed set, due to delays in embryo sac maturation. Reciprocal crosses indicate that these mutations acted sporophytically, and aniline blue staining and scanning electron microscopy showed that funicular and micropylar pollen tube guidance were disrupted. A PDIL2-1-yellow fluorescent protein fusion was mainly localized in the endoplasmic reticulum and was expressed in all tissues examined. In ovules, expression in integument tissues was much higher in the micropylar region in later developmental stages, but there was no expression in embryo sacs. We show that reduced seed set occurred when another copy of full-length PDIL2-1 or when enzymatically active truncated versions were expressed, but not when an enzymatically inactive version was expressed, indicating that these T-DNA insertion lines are gain-of-function mutants. Our results suggest that these truncated versions of PDIL2-1 function in sporophytic tissues to affect ovule structure and impede embryo sac development, thereby disrupting pollen tube guidance.

show abstract

Section: Introductionmentioning

confidence: 57%

“…However, in the sterile apetala mutant, megasporogenesis is arrested, although the development of the integuments are normal (Byzova et al, 1999). How sporophytic tissues influence female gametophyte development is still elusive (Yang and Sundaresan, 2000;Yadegari and Drews, 2004).…”

Section: Introductionmentioning

confidence: 99%

Truncation of a Protein Disulfide Isomerase, PDIL2-1, Delays Embryo Sac Maturation and Disrupts Pollen Tube Guidance inArabidopsis thaliana

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The smaller generative cell undergoes PMII and divides again, giving rise to two sperm cells. The female embryo sac develops from only one of the meiotic products and typically undergoes three successive syncytial divisions followed by cellularization into seven cells, including the two female gametes, namely the egg cell and the central cell ( Figure 1; [1]). …”

Section: Introductionmentioning

confidence: 99%

Endosperm: an integrator of seed growth and development

Berger

Grini

Schnittger

2006

Current Opinion in Plant Biology

165

107

View full text Add to dashboard Cite

“…Therefore, mutations affecting karyogamy should affect the female gametophyte and, as a consequence, should result in segregation distortion and reduced seed set (Drews and Yadegari, 2002). Using these criteria, we previously identified a large collection of female gametophyte mutants (Yadegari and Drews, 2004). Within this mutant collection were nine mutants with defects in fusion of the polar nuclei.…”

Section: Identification Of Nine Mutants Affected In Karyogamy Duringmentioning

confidence: 99%

NUCLEAR FUSION DEFECTIVE1 Encodes the Arabidopsis RPL21M Protein and Is Required for Karyogamy during Female Gametophyte Development and Fertilization

et al. 2006

View full text Add to dashboard Cite

Karyogamy, or nuclear fusion, is essential for sexual reproduction. In angiosperms, karyogamy occurs three times: twice during double fertilization of the egg cell and the central cell and once during female gametophyte development when the two polar nuclei fuse to form the diploid central cell nucleus. The molecular mechanisms controlling karyogamy are poorly understood. We have identified nine female gametophyte mutants in Arabidopsis (Arabidopsis thaliana), nuclear fusion defective1 (nfd1) to nfd9, that are defective in fusion of the polar nuclei. In the nfd1 to nfd6 mutants, failure of fusion of the polar nuclei is the only defect detected during megagametogenesis. nfd1 is also affected in karyogamy during double fertilization. Using transmission electron microscopy, we showed that nfd1 nuclei fail to undergo fusion of the outer nuclear membranes. nfd1 contains a T-DNA insertion in RPL21M that is predicted to encode the mitochondrial 50S ribosomal subunit L21, and a wildtype copy of this gene rescues the mutant phenotype. Consistent with the predicted function of this gene, an NFD1-green fluorescent protein fusion protein localizes to mitochondria and the NFD1/RPL21M gene is expressed throughout the plant. The nfd3, nfd4, nfd5, and nfd6 mutants also contain T-DNA insertions in genes predicted to encode proteins that localize to mitochondria, suggesting a role for this organelle in nuclear fusion.

show abstract

Female Gametophyte Development

Cited by 368 publications

References 86 publications

Truncation of a Protein Disulfide Isomerase, PDIL2-1, Delays Embryo Sac Maturation and Disrupts Pollen Tube Guidance inArabidopsis thaliana

Truncation of a Protein Disulfide Isomerase, PDIL2-1, Delays Embryo Sac Maturation and Disrupts Pollen Tube Guidance inArabidopsis thaliana

Endosperm: an integrator of seed growth and development

NUCLEAR FUSION DEFECTIVE1 Encodes the Arabidopsis RPL21M Protein and Is Required for Karyogamy during Female Gametophyte Development and Fertilization

Contact Info

Product

Resources

About