Feline low-grade intestinal T cell lymphoma: a unique natural model of human indolent T cell lymphoproliferative disorder of the gastrointestinal tract

Freiche, Valérie; Cordonnier, Nathalie; Paulin, Mathieu V; Huet, Hélène; Turba, María Elena; Macintyre, Elizabeth; Malamut, Georgia; Cerf‐Bensussan, Nadine; Molina, Thierry Jo; Hermine, Olivier; Bruneau, Julie; Couronné, Lucile

doi:10.1038/s41374-021-00581-x

Cited by 24 publications

(43 citation statements)

References 35 publications

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“…Recent studies indicate that low‐grade intestinal lymphoma is of a T‐cell phenotype 1,3,17 . In our LGITL cohort, the 22 cats were confirmed to have T‐cell low‐grade intestinal lymphoma.…”

Section: Discussionmentioning

confidence: 82%

“…Moreover, all LGITL and LPE cases were double‐blindly classified according to the 2016 revision of the WHO classification of lymphoma in humans and the WSAVA classification 8,9 . As previously mentioned, this data was recently validated for LGITL cats in an original comparative prospective study 17 …”

Section: Discussionmentioning

confidence: 99%

“…The cohort of cats diagnosed with LGITL in our study has already been compared to its counterpart in humans in another study 17 . The cohort of cats, subsequently diagnosed with LPE, was recruited for the present study.…”

Section: Methodsmentioning

confidence: 99%

“…Sections from each sample were labeled using a panel of antibodies. In a previous study, proper controls were performed for each of the antibodies 17 . Anti‐CD3 antibody staining was performed to identify the T‐cell lineage (1:20 dilution, F7.2.38, DakoCytomation, Santa Clara, California).…”

Section: Methodsmentioning

confidence: 99%

“…Finally, JAK/STAT pathway alterations recently have been reported in gastrointestinal T‐cell lymphoproliferative disorders (GI‐TLPDs) in humans 15,16 . Low‐grade intestinal T‐cell lymphoma recently has been confirmed as a relevant model for GI‐LPDs, highlighting the diagnostic value of phosphoSTAT5 17 …”

Section: Introductionmentioning

confidence: 99%

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Histopathologic, phenotypic, and molecular criteria to discriminate low‐grade intestinal T‐cell lymphoma in cats from lymphoplasmacytic enteritis

Freiche

Paulin

Cordonnier

et al. 2021

Veterinary Internal Medicne

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Background Differentiation of low‐grade intestinal T‐cell lymphoma (LGITL) from lymphoplasmacytic enteritis (LPE) in cats is a diagnostic challenge for pathologists. Objective Characterize histologic, immunohistochemical, and molecular features of LGITL and LPE. Animals Forty‐four client‐owned cats, 22 diagnosed with LGITL and 22 with LPE. Methods Prospective, cohort study. Clinical suspicion of LGITL or LPE was based on persistent gastrointestinal signs, unresponsive to empirical treatments. All cats underwent a standardized diagnostic evaluation, including biopsy (preferentially full‐thickness), and were diagnosed with LGITL or LPE after review of clinical, laboratory, sonographic, histologic, immunohistochemical, and clonality results. Results A monomorphic lymphocytic population (22/22, 100%) and in‐depth mucosal infiltration (15/22, 68%) were hallmarks of LGITL. Epithelial patterns (nests and plaques) were significantly more frequent in LGITL (11/22, 50%) than in LPE (1/22, 5%) cases (P = .001). A CD3+ lymphocytic apical‐to‐basal gradient was observed in 9/22 (41%) of LGITL vs 1/22 (5%) of LPE cases (P = .004). Most LPE cases (17/18, 94%) featured marked fibrosis in the superficial part of the lamina propria. The Ki‐67 20%‐ and 30%‐thresholds discriminated between LGITL and LPE within both the epithelium (specificity >95%) and lamina propria (specificity >95%), respectively. All LGITL cases were CD3+ pSTAT3− and pSTAT5+. T‐cell receptor gamma chain gene rearrangements indicated monoclonality in 86% of LGITL cases. Surprisingly, 70% of LPE cases featured monoclonality (40%) or monoclonality on a polyclonal background (30%). Conclusions and Clinical Importance We identified new histologic, immunohistochemical, and clonality criteria to distinguish LGITL from LPE.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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