Feline herpesvirus type 1 glycoproteins eliciting virus neutralizing and hemagglutination-inhibiting antibodies

Abstract

Monoclonal antibodies (MoAbs) to feline herpesvirus type 1 (FHV-1) were produced to identify the virus-specified immunogenic proteins. When antigens recognized by the MoAbs were investigated by immunoblot and immunoprecipitation assays using FHV-1-infected cell lysate, four groups of immunogenic proteins were identified. MoAbs directed against 60 kDa, 113 kDa, and 143 kDa/108 kDa glycoproteins had virus neutralizing activities, but those against 170 kDa protein did not. Furthermore, MoAbs to the 60 kDa glycoprotein also showed hemagglutination-inhibition activity, indicating that this glycoprotein might be the hemagglutinin.

Adhesion of Insect Cells Expressing the Feline Herpesvirus Type 1 Hemagglutinin(gD) to Feline Cell Lines.

“…3). However, the adhesion was not inhibited by MAbs 22F4 and 17C11 against FHV-1 gB and gC, respectively [7], when the MAbs were used as a control. These results indicated that FHV-1 gD might involve in restrictive adhesion between FHV-1 gD-expressing cells and cells susceptible to FHV-1 infection.…”

“…FHV-1 genes encoding seven glycoproteins, gB, gC, gD, gG, gH, gI and gE, were identified [14,17,18,21,22]. With the use of monoclonal antibodies (MAbs) against FHV-1, we identified three kinds of glycoproteins, gB, gC, and gD which induce virusneutralizing (VN) antibodies [7]. Especially, some of the MAbs against FHV-1 gD had hemagglutination (HA)-inhibition (HI) and complement-independent VN activities, and the gD expressed in Sf9 cells or affinity-purified gD induced VN antibody in mouse [7,13].…”

“…With the use of monoclonal antibodies (MAbs) against FHV-1, we identified three kinds of glycoproteins, gB, gC, and gD which induce virusneutralizing (VN) antibodies [7]. Especially, some of the MAbs against FHV-1 gD had hemagglutination (HA)-inhibition (HI) and complement-independent VN activities, and the gD expressed in Sf9 cells or affinity-purified gD induced VN antibody in mouse [7,13]. Therefore FHV-1 gD is thought to be one of the important candidates for a subunit vaccine against FHV-1 infection and to play an important role in FHV-1 infection.…”

“…On the other hand, we reported that the FHV-1 gD expressed in COS-7 cells or Spodoptera frugiperda (Sf9) cells possessed both HA and hemadsorption activities against feline red blood cells (RBC), but not against murine RBC [16,18] infected Sf9 cells were preincubated with an MAb 25C9 which recognized FHV-1 gD and possessed VN and HI activities [7] for 30 min at 4°C before placing on the CRFK cell monolayer, the adhesion was specifically inhibited by the MAb (Fig. 3).…”

Identification of the feline herpesvirus type 1 (FHV-1) genes encoding glycoproteins G, D, I and E: expression of FHV-1 glycoprotein D in vaccinia and raccoon poxviruses

“…As is typical of other herpesviruses, numerous FHV-1 glycoproteins are synthesized and then incorporated into membranes of infected cells and the virion envelope (Fargeaud et al, 1984;Maes et al, 1984;Limcumpao et al, 1990). These glycoproteins have been well characterized and are conserved among related herpesviruses.…”