Feline and Canine Coronaviruses: Common Genetic and Pathobiological Features

Poder, Sophie Le

doi:10.1155/2011/609465

Cited by 61 publications

(68 citation statements)

References 112 publications

(139 reference statements)

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“…Such mutations could not be detected in ORF3c of FECV samples. It has been shown before that an intact 3c gene is considered to be essential for efficient FECV replication in the intestinal tract, but dispensable for systemic FIPV replication (Chang et al, 2010;Le Poder, 2011;Pedersen et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Mutations of 3c and spike protein genes correlate with the occurrence of feline infectious peritonitis

Bank-Wolf

Stallkamp

Wiese

et al. 2014

Veterinary Microbiology

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The genes encoding accessory proteins 3a, 3b, 3c, 7a and 7b, the S2 domain of the spike (S) protein gene and the membrane (M) protein gene of feline infectious peritonitis virus (FIPV) and feline enteric coronavirus (FECV) samples were amplified, cloned and sequenced. For this faeces and/or ascites samples from 19 cats suffering from feline infectious peritonitis (FIP) as well as from 20 FECV-infected healthy cats were used. Sequence comparisons revealed that 3c genes of animals with FIP were heavily affected by nucleotide deletions and point mutations compared to animals infected with FECV; these alterations resulted either in early termination or destruction of the translation initiation codon. Two ascites-derived samples of cats with FIP which displayed no alterations of ORF3c harboured mutations in the S2 domain of the S protein gene which resulted in amino acid exchanges or deletions. Moreover, changes in 3c were often accompanied by mutations in S2. In contrast, in samples obtained from faeces of healthy cats, the ORF3c was never affected by such mutations. Similarly ORF3c from faecal samples of the cats with FIP was mostly intact and showed only in a few cases the same mutations found in the respective ascites samples. The genes encoding 3a, 3b, 7a and 7b displayed no mutations linked to the feline coronavirus (FCoV) biotype. The M protein gene was found to be conserved between FECV and FIPV samples. Our findings suggest that mutations of 3c and spike protein genes correlate with the occurrence of FIP.

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Section: Discussionmentioning

confidence: 99%

Mutations of 3c and spike protein genes correlate with the occurrence of feline infectious peritonitis

Bank-Wolf

Stallkamp

Wiese

et al. 2014

Veterinary Microbiology

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“…In the past decade, several researchers have studied the importance of CCoV as an agent of diarrhea in puppies Le Poder, 2011;Ntafis et al, 2013;Pratelli, 2011;Stavisky et al, 2012). In Brazil, despite the availability of safe and effective vaccines, CPV is considered the most common viral agent associated with enteritis in puppies (Castro et al, 2010b(Castro et al, , 2011.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of canine coronavirus strains circulating in Brazil

Costa

Castro

Bottino

et al. 2014

Veterinary Microbiology

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To characterize canine coronavirus (CCoV) circulating in diarrheic puppies in Brazil, 250 fecal samples collected between 2006 and 2012 were tested. By using RT-PCR to partially amplify the M gene, CCoV RNA was detected in 30 samples. Sequence analysis of the M protein grouped eight strains with CCoV-I and another 19 with CCoV-II prototypes. To genotype/subtype the CCoV strains and assess the occurrence of single or multiple CCoV infections, RT-PCR of the S gene was performed, and 25/30 CCoV-positive strains amplified with one or two primer pairs. For 17/25 samples, single infections were detected as follows: six CCoV-I, nine CCoV-IIa and two CCoV-IIb. Eight samples were positive for more than one genotype/subtype as follows: seven CCoV-I/IIa and one CCoV-I/IIb. Sequence analysis revealed that the CCoV-I and IIa strains shared high genetic similarity to each other and to the prototypes. The Brazilian strains of CCoV-IIb displayed an aminoacid insertion that was also described in CCoV-IIb-UCD-1 and TGEV strains. Among the 25 CCoV-positive puppies, five had a fatal outcome, all but one of which were cases of mixed infection. The current study is the first reported molecular characterization of CCoV-I, IIa and IIb strains in Brazil.

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“…Based on analysis of the M-and/or S-protein encoding genes, CCoV strains have been classified into at least two genotypes, CCoV-I and CCoV-II Buonavoglia, 2008, 2011). Recently, CCoV-II strains were further classified into two subtypes, CCoV-IIa (classical strains) and CCoV-IIb (strains arising from putative recombination events between CCoV-II and transmissible gastroenteritis virus of swine) Buonavoglia, 2008, 2011;Le Poder, 2011). CCoVs have been detected in canine populations worldwide Buonavoglia, 2008, 2011).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of canine parvovirus and canine enteric coronavirus in diarrheic dogs on the island of St. Kitts: First report from the Caribbean region

et al. 2017

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Although canine parvovirus (CPV) and canine enteric coronavirus (CCoV) are important enteric pathogens of dogs and have been studied extensively in different parts of the world, there are no reports on these viruses from the Caribbean region. During 2015-2016, a total of 104 diarrheic fecal samples were collected from puppies and adult dogs, with or without hemorrhagic gastroenteritis, on the Caribbean island of St. Kitts (KNA). By PCR, 25 (24%, n=104) samples tested positive for CPV. Based on analysis of the complete deduced VP2 amino acid sequences, 20 of the KNA CPV strains were assigned to new CPV-2a (also designated as CPV-2a-297A). On the other hand, the VP2 genes of the remaining 5 strains were partially characterized, or could not be sequenced. New CPV-2a was the predominant CPV variant in St. Kitts, contrasting the molecular epidemiology of CPV variants reported in most studies from nearby North and South American countries. By RT-PCR, CCoVs were detected in 5 samples (4.8%, n=104). Based on analysis of partial M-protein gene, the KNA CCoV strains were assigned to CCoV-I genotype, and were closely related to CCoV-I strains from Brazil. To our knowledge, this is the first report on detection and genetic diversity of CPV and CCoV in dogs from the Caribbean region, and underscores the importance of similar studies in the other Caribbean islands.

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Feline and Canine Coronaviruses: Common Genetic and Pathobiological Features

Cited by 61 publications

References 112 publications

Mutations of 3c and spike protein genes correlate with the occurrence of feline infectious peritonitis

Mutations of 3c and spike protein genes correlate with the occurrence of feline infectious peritonitis

Molecular characterization of canine coronavirus strains circulating in Brazil

Molecular characterization of canine parvovirus and canine enteric coronavirus in diarrheic dogs on the island of St. Kitts: First report from the Caribbean region

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