Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA

Rossen, John W. A.; Kouamé, Jérôme; Goedheer, Anneke; Vennema, Harry; Rottier, Peter J. M.

doi:10.1007/s007050170147

Cited by 14 publications

(13 citation statements)

References 17 publications

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“…This finding is consistent with a localized infection restricted in the intestine. Several studies have investigated the interaction of coronaviruses and other viruses with epithelial cells using a similar approach (Ren et al, 2006;Rossen et al, 1994;Wang et al, 2000;Jia et al, 2005;Tseng et al, 2005;Rossen et al, 2001Rossen et al, , 1995Rossen et al, , 1996Pratelli, 2011;Tao et al, 2013;Fuller et al, 1984). Though entry into and egress from polarized cells for several coronaviruses is restricted to the apical plasma membrane, other coronaviruses show a different pattern (see Introduction).…”

Section: Discussionmentioning

confidence: 99%

“…TGEV, HCoV-229E and severe acute respiratory syndrome associated coronavirus (SARS-CoV) (Ren et al, 2006;Rossen et al, 1994;Wang et al, 2000;Jia et al, 2005;Tseng et al, 2005). Feline coronavirus (FCoV) and mouse hepatitis coronavirus (MHV) mediated apical entry and basolateral release in polarized epithelial cells (Rossen et al, 2001(Rossen et al, , 1995(Rossen et al, , 1996. The recently identified coronavirus, Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and also the canine coronavirus (CCoV) enter and exit at both sites of polarized epithelial cells (Pratelli, 2011;Tao et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Cong

Bai

et al. 2015

Virology

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a b s t r a c tInfection of polarized intestinal epithelial cells by porcine epidemic diarrhea virus (PEDV) was characterized. Indirect immunofluorescence assay, real-time PCR, and transmission electron microscopy confirmed PEDV can be successfully propagated in immortalized swine small intestine epithelial cells (IECs). Infection involved porcine aminpeptidase N (pAPN), a reported cellular receptor for PEDV, transient expression of pAPN and siRNA targeted pAPN increased and decreased the infectivity of PEDV in IECs, respectively. Subsequently, polarized entry into and release from both Vero E6 and IECs was analyzed. PEDV entry into polarized cells and pAPN grown on membrane inserts occurs via apical membrane. The progeny virus released into the medium was also quantified which demonstrated that PEDV is preferentially released from the apical membrane. Collectively, our data demonstrate that pAPN, the cellular receptor for PEDV, mediates polarized PEDV infection. These results imply the possibility that PEDV infection may proceed by lateral spread of virus in intestinal epithelial cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Cong

Bai

et al. 2015

Virology

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“…This implies that there is frequent interspecies circulation of either CCoV in cats or FCoV in dogs, since mixed infections are required to give rise to recombination. Where the recombination takes place is unknown, but it is known that CCoV is able to use the feline aminopeptidase (fAPN) glycoprotein as a cellular receptor [36,42] and that, under experimental conditions, cats can be infected with CCoV [2]. Moreover, the possibility that FCoV might infect dogs cannot be ruled out.…”

Section: Canine Coronavirus Genomic Structure and Evolutionary Changesmentioning

confidence: 99%

Genetic evolution of canine coronavirus and recent advances in prophylaxis

Pratelli¹

2006

Vet. Res.

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-Since the first identification of the virus in 1971, the disease caused by canine coronavirus (CCoV) has not been adequately investigated and the role that the virus plays in canine enteric illness has still not been well established. In the last decade, as a consequence of the relatively high mutation frequency of RNA positive stranded viruses, CCoV has evolved and a new genotype has been identified in the faeces of infected dogs. The several studies carried out by different researchers have focused upon the epidemiological relevance of these viruses and, considering the wide diffusion of CCoV infections among dog populations, the author underlines the need for further investigation on the biology of CCoV and on the pathogenetic role of their infections.

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“…Feline FCWF cells (obtained from N.C. Pedersen, UC Davis, Davis, USA) were used for the antiviral experiments with, and propagation of, FCoV serotype II FIPV (strain 79-1146), FCoV (strain 79-1683; obtained from J. Evermann, Washington State University, USA; McKeirnan et al, 1981) and FIPV-3abcFL (de Haan et al, 2005) and the FCoV serotype I FIPV Black TN406HP (Pedersen and Black, 1983; obtained from N.C. Pedersen, UC Davis, Davis, USA). Mouse LR7 cells, a L-2 murine fibroblast cell line stably expressing the MHV receptor (Rossen et al, 2001) were used for the experiments with, and propagation of, MHV (strain A59; American Type Culture Collection, ATCC) and MHV-EFLM (de Haan et al, 2003).…”

Section: Cells Virusesmentioning

confidence: 99%

Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture

et al. 2007

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Coronaviruses are important human and animal pathogens, the relevance of which increased due to the emergence of new human coronaviruses like SARS-CoV, HKU1 and NL63. Together with toroviruses, arteriviruses, and roniviruses the coronaviruses belong to the order Nidovirales. So far antivirals are hardly available to combat infections with viruses of this order. Therefore, various antiviral strategies to counter nidoviral infections are under evaluation. Lectins, which bind to N-linked oligosaccharide elements of enveloped viruses, can be considered as a conceptionally new class of virus inhibitors. These agents were recently evaluated for their antiviral activity towards a variety of enveloped viruses and were shown in most cases to inhibit virus infection at low concentrations. However, limited knowledge is available for their efficacy towards nidoviruses. In this article the application of the plant lectins Hippeastrum hybrid agglutinin (HHA), Galanthus nivalis agglutinin (GNA), Cymbidium sp. agglutinin (CA) and Urtica dioica agglutinin (UDA) as well as non-plant derived pradimicin-A (PRM-A) and cyanovirin-N (CV-N) as potential antiviral agents was evaluated. Three antiviral tests were compared based on different evaluation principles: cell viability (MTT-based colorimetric assay), number of infected cells (immunoperoxidase assay) and amount of viral protein expression (luciferase-based assay). The presence of carbohydrate-binding agents strongly inhibited coronaviruses (transmissible gastroenteritis virus, infectious bronchitis virus, feline coronaviruses serotypes I and II, mouse hepatitis virus), arteriviruses (equine arteritis virus and porcine respiratory and reproductive syndrome virus) and torovirus (equine Berne virus). Remarkably, serotype II feline coronaviruses and arteriviruses were not inhibited by PRM-A, in contrast to the other viruses tested.

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Feline and canine coronaviruses are released from the basolateral side of polarized epithelial LLC-PK1 cells expressing the recombinant feline aminopeptidase-N cDNA

Cited by 14 publications

References 17 publications

Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Porcine aminopeptidase N mediated polarized infection by porcine epidemic diarrhea virus in target cells

Genetic evolution of canine coronavirus and recent advances in prophylaxis

Antiviral activity of carbohydrate-binding agents against Nidovirales in cell culture

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