Feeding preference of the sand flies Lutzomyia umbratilis and L. spathotrichia (diptera: Psychodidae, Phlebotominae) in an urban forest patch in the city of Manaus, Amazonas, Brazil

Nery, Liliane Coelho da Rocha; Lorosa, Nery Elias S; Franco, Antônia Maria Ramos

doi:10.1590/s0074-02762004000600006

Cited by 28 publications

(7 citation statements)

References 10 publications

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“…guyanensis DNA; however, it cannot be ascertained whether these two parasites were within the same phlebotomine or in different specimens of that pool. In this study and in the literature [53–56] it is convergent with the possibility of N . umbratilis feed on different animals that act as potential reservoirs of Leishmania spp.…”

Section: Discussionsupporting

confidence: 83%

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Leishmania DNA detection and species characterization within phlebotomines (Diptera: Psychodidae) from a peridomicile-forest gradient in an Amazonian/Guianan bordering area

et al. 2019

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In the border region between Brazil and French Guiana, American cutaneous leishmaniasis is a worrisome public health issue, and entomological studies are required there to better identify classical and putative emerging transmission patterns. The present study aimed to detect and characterize Leishmania DNA in the phlebotomine population of Oiapoque (Amapá State, Brazil). Phlebotomines were captured in anthropized and wild environments in the outskirts of Oiapoque municipality, using CDC light traps installed in vertical (ground/canopy level) and horizontal (peridomicile/extradomicile/forest-edge/forest) strata. Captured specimens were identified according to their morphology. Females were processed for Leishmania DNA detection and characterization using a multiplex polymerase chain reaction targeting kinetoplast DNA (kDNA) and the phlebotomine cacophony gene. The kDNA positive samples were characterized by cloning and sequencing the Leishmania 234 bp- hsp70 gene. Among the 3957 phlebotomine specimens captured, 26 pooled female samples were positive for Leishmania ( Viannia ) spp. DNA. Sequencing analysis allowed species-specific identification of L . ( V .) braziliensis DNA in Trichophoromyia ininii , Bichromomyia flaviscutellata , Nyssomyia umbratilis , and Evandromyia infraspinosa , and L . ( V .) guyanensis DNA in Ny . umbratilis . A pooled sample of Ny . umbratilis was positive for both L . ( V .) braziliensis and L . ( V .) guyanensis DNA. The present study provided additional information regarding ACL ecology in Oiapoque, highlighting the presence of L . ( V .) braziliensis DNA in different phlebotomine species. The epidemiological implications of these findings and the determinant incrimination of L . ( V .) braziliensis as proven vectors in that region must be clarified. In this regard, studies on Leishmania spp. infection and suggestive anthropophilic behavior of associated phlebotomines need to be prioritized in entomological surveillance.

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Section: Discussionsupporting

confidence: 83%

“…flaviscutellata [52] and N . umbratilis has been found alternatively feeding on blood from rodents in an environmentally-impacted area close to Manaus, Amazonian Brazil [53]. Additionally, E .…”

Section: Discussionmentioning

confidence: 99%

Leishmania DNA detection and species characterization within phlebotomines (Diptera: Psychodidae) from a peridomicile-forest gradient in an Amazonian/Guianan bordering area

et al. 2019

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“…Tiny volumes of blood meals together with variations in blood digestion and defecation pace pose complications to successful host identification. First methods used for blood source determination were serological techniques like precipitin test [9,10] or ELISA [11,12], which is still used nowadays. Later, DNA-based molecular methods were developed and are widely applied since; for phlebotomine sand flies the most often used is sequencing of cytochrome b (cyt b) gene [13,14], although this approach is laborious and quite expensive.…”

Section: Introductionmentioning

confidence: 99%

A novel MALDI-TOF MS-based method for blood meal identification in insect vectors: A proof of concept study on phlebotomine sand flies

et al. 2019

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BackgroundIdentification of blood sources of hematophagous arthropods is crucial for understanding the transmission cycles of vector-borne diseases. Many different approaches towards host determination were proposed, including precipitin test, ELISA, DNA- and mass spectrometry-based methods; yet all face certain complications and limitations, mostly related to blood degradation. This study presents a novel method for blood meal identification, peptide mass mapping (PMM) analysis of host-specific hemoglobin peptides using MALDI-TOF mass spectrometry.Methodology/Principal findingsTo identify blood meal source, proteins from abdomens of engorged sand fly females were extracted, cleaved by trypsin and peptide fragments of host hemoglobin were sequenced using MALDI-TOF MS. The method provided correct host identification of 100% experimentally fed sand flies until 36h post blood meal (PBM) and for 80% samples even 48h PBM. In females fed on two hosts, both blood meal sources were correctly assigned for 60% of specimens until 36h PBM. In a validation study on field-collected females, the method yielded unambiguous host determination for 96% of specimens. The suitability of PMM-based MALDI-TOF MS was proven experimentally also on lab-reared Culex mosquitoes.Conclusions/SignificancePMM-based MALDI-TOF MS analysis targeting host specific hemoglobin peptides represents a sensitive and cost-effective method with a fast and simple preparation protocol. As demonstrated here on phlebotomine sand flies and mosquitoes, it allows reliable and rapid blood source determination even 48h PBM with minimal material input and provides more robust and specific results than other currently used methods. This approach was also successfully tested on field-caught engorged females and proved to be a promising useful tool for large-scale screening of host preferences studies. Unlike other methods including MALDI-TOF protein profiling, it allows correct identification of mixed blood meals as was demonstrated on both experimentally fed and field-collected sand flies.

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“…(continued) 2010,Rojas-Ahumada et al 2012), but most exhibit anthropogenic alterations(Rocha et al 2004, Ramos et al 2014.…”

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confidence: 99%

Under the light: high prevalence of haemoparasites in lizards (Reptilia: Squamata) from Central Amazonia revealed by microscopy

Picelli

Ramires

Masseli

et al. 2020

An. Acad. Bras. Ciênc.

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Blood samples from 330 lizards of 19 species were collected to investigate the occurrence of haemoparasites. Samplings were performed in areas of upland (terrafi rme) forest adjacent to Manaus municipality, Amazonas, Brazil. Blood parasites were detected in 220 (66%) lizards of 12 species and comprised four major groups: Apicomplexa (including haemogregarines, piroplasms, and haemosporidians), trypanosomatids, microfi larid nematodes and viral or bacterial organisms. Order Haemosporida had the highest prevalence, with 118 (35%) animals from 11 species. For lizard species, Uranoscodon superciliosus was the most parasitised host, with 103 (87%; n = 118) positive individuals. This species also presented the highest parasite diversity, with the occurrence of six taxa. Despite the diffi culties attributed by many authors regarding the use of morphological characters for taxonomic resolution of haemoparasites, our lowcost approach using light microscopy recorded a high prevalence and diversity of blood parasite taxa in a relatively small number of host species. This report is the fi rst survey of haemoparasites in lizards in the study region. It revealed a high diversity of lizard haemoparasites and highlights the need to understand their impacts on hosts.

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Feeding preference of the sand flies Lutzomyia umbratilis and L. spathotrichia (diptera: Psychodidae, Phlebotominae) in an urban forest patch in the city of Manaus, Amazonas, Brazil

Cited by 28 publications

References 10 publications

Leishmania DNA detection and species characterization within phlebotomines (Diptera: Psychodidae) from a peridomicile-forest gradient in an Amazonian/Guianan bordering area

Leishmania DNA detection and species characterization within phlebotomines (Diptera: Psychodidae) from a peridomicile-forest gradient in an Amazonian/Guianan bordering area

A novel MALDI-TOF MS-based method for blood meal identification in insect vectors: A proof of concept study on phlebotomine sand flies

Under the light: high prevalence of haemoparasites in lizards (Reptilia: Squamata) from Central Amazonia revealed by microscopy

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