Feeding on frozen live yeast has some deleterious effects in Drosophila melanogaster

Abstract

Colinet. Feeding on frozen live yeast has some deleterious effects in Drosophila melanogaster. Experimental Gerontology, Elsevier, 2015, 69, pp.202-210. 10 severe stresses and behaviour in Drosophila melanogaster flies. The present study tested whether feeding flies with frozen yeast rather than with fresh yeast could have some effect on these traits, the other components of the food being similar in the two groups. Freezing altered live yeast quality and flies feeding on frozen yeast lived slightly less (males), were less fecund at older ages, and poorly resisted to some severe stresses (cold and starvation), no negative effect being observed on resistance to heat. It seems that, like in humans, feeding on a low quality food can negatively impact healthspan and that an appropriate food is not only a food with optimal number of calories and appropriate ratios of proteins, carbohydrates, and fat.

Dietary nutrient balance shapes phenotypic traits of Drosophila melanogaster in interaction with gut microbiota

“…Our restriction was more severe than that of Burger et al (2007) who used a restrictive diet comparable to our intermediate diet (8:8), which could explain this result. The lower knockdown temperature in yeast-rich and sugar-poor diet 2:16 diet is consistent with previous observations that excess in dietary sugar is not necessarily beneficial for cold tolerance (Colinet et al, 2013) and that yeasts, especially live ones, favor cold tolerance (Colinet and Renault, 2014;Le Bourg et al, 2015). Although increased cold tolerance of microbiota-altered flies is surprising , it could be linked to the unexpected increased yeast gut content that resulted from our antibiotic treatment.…”

“…The focus is often on bacteria as the main gut symbionts but the role of yeasts should not be neglected (Hoang et al, 2015). Live yeasts can affect Drosophila physiology and increase survival to infections and starvation, in addition to cold stress (Le Bourg et al, 2015;Le Rohellec and Le Bourg, 2009). The difference between the effects of dead and live yeasts suggests these microorganisms provide an active protection that goes beyond being a simple food source.…”

Yeast quality in juvenile diet affects Drosophila melanogaster adult life traits

“…melanogaster studies have shown that the quality of the fungal diet, provided either throughout life or only at the adult age, can affect adult life traits. For example, adults fed with frozen or heat-killed yeast showed altered development, lower fertility, shorter life span and increased sensitivity to stress than did adults fed on a fresh yeast diet 11 . Furthermore these effects persisted even when a 600-fold increased amount of heat-killed yeast was added to the diet 12 .…”

Is lifespan linked with developmental viability in Drosophila melanogaster?

“…1 in Le Bourg, 2021a) were however not used to observe lifespan. Flies used in the present article were the control groups of several articles observing the effect of various factors on lifespan (Le Bourg, 2007Bourg, , 2010Bourg, , 2011Bourg, , 2012Bourg, , 2021bLe Bourg and Lints, 1989;Le Bourg and Medioni, 1991;Le Bourg et al, 2000, 2002, 2015Le Rohellec and Le Bourg, 2009), and were subjected to the very same rearing conditions (see below). The mean viability in the present study was 74.42% (26 groups, range: 41.37-87.09%).…”