Feeding effects of low-level fish meal replacement by algal meals of Schizochytrium limacinum and Nannochloropsis salina on largemouth bass (Micropterus salmoides)

Liao, Hongping; Liu, Peiqin; Yongyan, Deng; Zhang, Wenqi; Pan, Ciguang; Jia, Youming; Long, Feiping

doi:10.1016/j.aquaculture.2022.738311

Cited by 18 publications

(15 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whereas HDL-C removes TC from the blood and sedimentation in the liver, which is then excreted from the animal, LDL-C plays an important part in the immune system of shrimp (Yue et al, 2012;Chen et al, 2018). The current research showed a decrease in HDL-C and LDL-C levels and a significant decrease in HDL-C in the hemolymph of Litopenaeeus vannamei fed the LF diet, which is different from the results hemolymph in Micropterus salmoides fed low-level fish meal diet supplemented with 4% S. Limacinum (Liao et al, 2022). Reasons for this may be the differences in diet composition, species, and amount of S. Limacinum supplementation.…”

Section: Figurecontrasting

confidence: 77%

“…In addition, there have been several studies showing that dietary supplementation of S. Limacinum be significantly enhanced the non-specific immunity of Trachinotus Ovatus (Xie et al, 2019) and improve the intestinal health of Oreochromis niloticus (Souza et al, 2020) and Oncorhynchus mykiss (Lyons et al, 2016). The results of another study suggest that S. Limacinum can be a promising low-level substitute for fish meals, which could improve the fillet texture of largemouth bass (Liao et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Nonetheless, the high cost of fish meal has increased the cost of feed Previous studies found that fish meal in aquatic animal feed can be substituted by different protein sources, such as soy protein peptide (Lin et al, 2022), soy protein concentrate (Zhu et al, 2020), fermented soy pulp (Kari et al, 2022), hydrolyzed fish protein powder (Hlordzi et al, 2022), bacterial protein meal (Chen et al, 2021b), concentrated dephenolization cottonseed protein (Zhao et al, 2021), blood meal (Kirimi et al, 2016), black soldier fly (Huang et al, 2022), meat and bone meal (Ai et al, 2006), and hydrolyzed feather meal (Campos et al, 2017). Plant proteins are widely available and inexpensive, which were favored by researchers for partial replacement of fish meal in aquatic animal feed (Liao et al, 2022). However, plant protein generally has the disadvantages of amino acid imbalance and high content of anti-nutritional factors (Jannathulla et al, 2019), and adding excessive plant protein can also affect the growth performance, intestinal microecology, and nutrition metabolism of aquatic animals (Ray et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Schizochytrium limacinum supplementation to a low fish-meal diet on growth performance, lipid metabolism, apoptosis, autophagy and intestinal histology of Litopenaeus vannamei

Yao

Lin²,

Shi³

et al. 2022

Front. Mar. Sci.

View full text Add to dashboard Cite

In this experiment, we aimed to evaluate the relationship between the addition of Schizochytrium limacinum to low fish meal diets on growth performance, apoptosis, autophagy, lipid metabolism, and intestinal health of Lipenaeus vanamei. The diet containing 25% fish meal was used as a positive control (FM) and the other three diets contained 15% fish meal and were supplemented with 0, 0.3, and 0.6% S. Limacinum (LF, LFLD, LFHD). The shrimp (0.22 ± 0.00 g) were divided into four replicates of 40 shrimp per tank and fed four times daily to apparent satiation for 8 weeks. Results showed that the final weight (FBW) and weight gain rate (WGR) of shrimp fed FM and LFHD diets were significantly increased compared to those fed the LFLD diet (P<0.05), and there was no significant difference in survival rate (SR) and feed conversion rate (FCR) among the groups (P>0.05). Supplementation of S. Limacinum in low fish meal diets had no effects on shrimp body composition (P<0.05). There were significant differences (P<0.05) in low-density lipoprotein (LDL-C) glucose (GLU), triglycerides (TG), and total cholesterol (TC) in the hemolymph of shrimp fed the LF diet compared to those fed the LFLD and LFHD diets. HE staining and transmission electron microscopy (TEM) results showed that the microvilli height, mucosal folds height, mucosal folds width and muscle layer thickness in the intestine of shrimp fed the LF diet were significantly reduced compared to those fed the other three diets (P<0.05). Swelling of the endoplasmic reticulum and irregular mitochondria in the gut of shrimp fed the LF diet was also observed by TEM, and the endoplasmic reticulum and mitochondria of shrimp fed the LFHD diet returned to a healthy state. Hepatopancreas genes expression results were showed that the gene expression of 5′ -AMP-activated protein kinase (ampk), stearoyl-CoA desaturase (scd1), acetyl-CoA carboxylase 1 (acc1), and malonyl-CoA decarboxylase (mcd) of shrimp fed the LF diet was significantly increased compared to those fed the FM diet (P<0.05). The gene expression of sterol regulatory element-binding protein (srbep) and carnitine palmitoyl transferase 1 (cpt-1) of shrimp fed the LFLD diet was significantly increased compared to those fed the LF diet (P<0.05). The gene expression of acc1, mcd and scd1 of shrimp fed the LFHD diet was significantly reduced compared to those fed the LF diet (P<0.05). Results of genes expression associated with apoptosis in the hepatopancreas showed that the gene expression of B lymphocytoma-2 (bcl-2), BCL2 associated X apoptosis regulator (bax) and cysteinyl aspartate specific proteinase 8 (caspase 8) of shrimp fed the LF diet was significantly reduced compared to those fed the FM diet (P<0.05). The gene expression of bcl-2 of shrimp fed the LFHD diet was significantly reduced compared to those fed the LF diet (P<0.05). Genes related to autophagy in the hepatopancreas showed that the expression of autophagy-related protein 12 (atg 12), autophagy-related protein 13 (atg 13) and beclin1 of shrimp fed LF the diet was significantly reduced compared to those fed the FM diet (P<0.05). The gene expression of atg 12 and atg 13 of shrimp fed the LFHD diet was significantly increased compared to those fed the LF diet (P<0.05). In summary, reducing fish meal is detrimental to the growth performance and intestinal health of shrimp, and 0.6% S. Limacinum supplementation can improve the growth performance, promotes hepatopancreas lipid metabolism, reduces apoptosis, promotes autophagy and improve intestinal health of Litopenaeus vannamei.

show abstract

Section: Figurecontrasting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Schizochytrium limacinum supplementation to a low fish-meal diet on growth performance, lipid metabolism, apoptosis, autophagy and intestinal histology of Litopenaeus vannamei

Yao

Lin²,

Shi³

et al. 2022

Front. Mar. Sci.

View full text Add to dashboard Cite

show abstract

“…These results are consistent with other published works when FM was replaced by mealworm ( Tenebrio molitor ) meal in hybrid grouper [ 64 ], MBM in Ussuri catfish [ 65 ], BM in black carp ( Mylopharyngodon piceus ) [ 66 ] and silkworm pupae meal in Jian carp ( Cyprinus carpio ) [ 67 ]. However, no change in the antioxidant activities of fish was observed with FM replacement with black soldier fly [ 68 ] and algal meals of Schizochytrium limacinum and Nannochloropsis salina [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

Fish Meal Replacement and Early Mild Stress Improve Stress Responsiveness and Survival of Fish after Acute Stress

Zare

Kazempour

Hosseini

et al. 2023

Animals

View full text Add to dashboard Cite

Stress responsiveness and fish meal (FM) replacement are two of the most important concerns toward achieving sustainable aquaculture. The purpose of this study was to see how early mild stress (netting) and FM replacement with meat and bone meal (MBM) affected oscar (Astronotus ocellatus; 5.2 ± 0.9 g) growth, hematology, blood biochemistry, immune responses, antioxidant system, liver enzymes, and stress responses. Oscars were subjected to a 3 × 3 experimental design (three fish meal replacement levels: 250, 180 and 110 g/kg of FM in diets; three stress periods: 0-, 2- and 3-times early mild stress). After ten weeks of the experiment, FM levels in diets did not affect growth data, but the survival rate after the acute confinement (AC) stress was lower in 11FM treatments (47.7% compared to 67.7%) than others. Fish exposed to the 3Stress schedule had a lower growth (31.03 ± 6.50 g) and survival rate (55.5%) after the AC stress than the 2Stress group (38.92 ± 6.82 g and 70.0%). Lower survival and growth rate in the 3Stress and 11FM groups coincided with the lowest blood performance, total protein, lysozyme, complement C4, complement C3, immunoglobulin, superoxide dismutase, catalase, glutathione peroxidase, and the highest glucose, cortisol, low-density lipoprotein and aspartate aminotransferase serum levels. Altogether, this study revealed that it is possible to replace FM with MBM up to 28% (180 g/kg of FM) without negative effects on the growth and health of juvenile oscar as dietary 110 g/kg of FM impaired fish health. While fish welfare should be considered, we can conclude that mild stress (2Stress) during the farming period, but without adding excessive alternative protein sources, can improve the stress responsiveness of oscar.

show abstract

“…Liao et al . (2022) investigated the effects of low‐level replacement (4%) of dietary FM by S. limacinum and N. salina individually and in combination in the diet of juvenile LMB (IBW: 8.72 ± 0.10 g), and demonstrated that S. limacinum alone or in combination with N. salina increased the n‐3 to n‐6 ratio of polyunsaturated fatty acids in the liver and muscle, without negative effect on growth performance. Collectively, algae‐based protein possess excellent nutritional and functional properties, which exhibits a great potential as FM substitution for LMB.…”

Section: Single‐cell Proteinmentioning

confidence: 99%

Alternative proteins as fish meal substitution in diets for largemouth bass (Micropterus salmoides): a mini review

Cheng

Zhu

et al. 2023

Int J of Food Sci Tech

View full text Add to dashboard Cite

SummaryAquaculture has been challenged to explore alternative proteins to develop efficient feed formulations that foster a sustainable future growth. Largemouth bass (LMB) are economically important cultured fish with a high demand for protein. One of the traditional ingredients used to develop protein diets is fish meal (FM). With the aquaculture industry expanding, the FM price climbs with broadening the imbalance between the supply and requirement. Hence, there is a requisite to explore novel alternative proteins sources. In this review, we summarised recent knowledge about the alternative sources of proteins with potential to partially or fully substitute FM in LMB feed and the shortcomings of present alternative proteins. We also highlight the challenges faced for LMB feed in the substitution of FM with alternative proteins, which need more in‐depth investigations in the future.

show abstract

Feeding effects of low-level fish meal replacement by algal meals of Schizochytrium limacinum and Nannochloropsis salina on largemouth bass (Micropterus salmoides)

Cited by 18 publications

References 49 publications

Effect of Schizochytrium limacinum supplementation to a low fish-meal diet on growth performance, lipid metabolism, apoptosis, autophagy and intestinal histology of Litopenaeus vannamei

Effect of Schizochytrium limacinum supplementation to a low fish-meal diet on growth performance, lipid metabolism, apoptosis, autophagy and intestinal histology of Litopenaeus vannamei

Fish Meal Replacement and Early Mild Stress Improve Stress Responsiveness and Survival of Fish after Acute Stress

Alternative proteins as fish meal substitution in diets for largemouth bass (Micropterus salmoides): a mini review

Contact Info

Product

Resources

About