Feedback regulation mode of gene circuits directly affects the detection range and sensitivity of lead and mercury microbial biosensors

Du, Ruoxi; Guo, Mingzhang; He, Xiaoyun; Huang, Kunlun; Luo, Yunbo; Xu, Wentao

doi:10.1016/j.aca.2019.08.006

Cited by 29 publications

(18 citation statements)

References 29 publications

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“…It is well known that silver chloride is insoluble in water, and chlorizated salts formed by other metal ions except for mercury is completely ionized and dissolved in the aqueous solution. Although mercury chloride has a tendency to exist in its molecular form in aqueous solution, bioavailable Hg(II) can still be well sensed by whole-cell biosensors (Du et al 2019;Zhang et al 2021). To avoid the possible effect of sodium chloride involved in LB culture medium on the performance of two developed biosensors, the specificity of indigoidinebased biosensors was further investigated in SLB culture medium.…”

Section: Responses Of the Indigoidine-based Biosensors Incubated In Lb Culture Medium Toward Different Heavy Metal Ionsmentioning

confidence: 99%

Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor

Hui¹,

Guo

Li³

et al. 2021

Appl Microbiol Biotechnol

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During the last few decades, whole-cell biosensors have attracted increasing attention for their enormous potential in monitoring bioavailable heavy metal contaminations in the ecosystem. Visual and measurable output signals by employing natural pigments have been demonstrated to offer another potential choice to indicate the existence of bioavailable heavy metals in recent years. The biosynthesis of the blue pigment indigoidine has been achieved in E. coli following heterologous expression of both BpsA (a single-module non-ribosomal peptide synthetase) and PcpS (a PPTase to activate apo-BpsA). Moreover, we demonstrated herein the development of the indigoidine-based whole-cell biosensors to detect bioavailable Hg(II) and Pb(II) in water samples by employing metal-responsive transcriptional regulator MerR and PbrR as the sensory elements, and the indigoidine biosynthesis gene cluster as a reporter element. The resulting indigoidine-based biosensors presented a good selectivity and high sensitivity to target metal ions. High concentration of target metal exposure could be clearly recognized by the naked eye due to the color change by the secretion of indigoidine, and quantified by measuring the absorbance of the culture supernatants at 600 nm. Doseresponse relationships existed between the exposure concentrations of target heavy metals and the production of indigoidine. Although fairly good linear relationships were obtained in a relatively limited concentration range of the concentrations of heavy metal ions, these findings suggest that genetically controlled indigoidine biosynthesis triggered by the MerR family transcriptional regulator can enable a sensitive, visual, and qualitative whole-cell biosensor for bioindicating the presence of bioaccessible heavy metal in environmental water samples. Key points• Biosynthesis pathway of indigoidine reconstructed in a high copy number plasmid in E. coli.• Visual and colorimetric detection of Hg(II) and Pb(II) by manipulation of indigoidine biosynthesis through MerR family metalloregulator. •Enhanced detection sensitivity toward Hg(II) and Pb(II) achieved using novel pigment-based whole-cell biosensors.

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Section: Responses Of the Indigoidine-based Biosensors Incubated In Lb Culture Medium Toward Different Heavy Metal Ionsmentioning

confidence: 99%

Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor

Hui¹,

Guo

Li³

et al. 2021

Appl Microbiol Biotechnol

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“…Jia et al have reported that the rearrangement of the genetic circuit of the lead resistance operon pbr improved the lead sensitivity by about ten times [68]. Moreover, it has also been reported that the detection range and sensitivity to analytes are modulated by the feedback regulation of genetic circuits [69]. Most of the whole-cell biosensors reported employ genetic systems responsive to external stimuli, including heavy metals, chemicals, and other environmental changes.…”

Section: Bacterial Cell-based Biosensorsmentioning

confidence: 99%

Section: Transcription Factor-based Biosensorsmentioning

confidence: 99%

Transcription Factor-Based Biosensors for Detecting Pathogens

et al. 2022

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Microorganisms are omnipresent and inseparable from our life. Many of them are beneficial to humans, while some are not. Importantly, foods and beverages are susceptible to microbial contamination, with their toxins causing illnesses and even death in some cases. Therefore, monitoring and detecting harmful microorganisms are critical to ensuring human health and safety. For several decades, many methods have been developed to detect and monitor microorganisms and their toxicants. Conventionally, nucleic acid analysis and antibody-based analysis were used to detect pathogens. Additionally, diverse chromatographic methods were employed to detect toxins based on their chemical and structural properties. However, conventional techniques have several disadvantages concerning analysis time, sensitivity, and expense. With the advances in biotechnology, new approaches to detect pathogens and toxins have been reported to compensate for the disadvantages of conventional analysis from different research fields, including electrochemistry, nanotechnology, and molecular biology. Among them, we focused on the recent studies of transcription factor (TF)-based biosensors to detect microorganisms and discuss their perspectives and applications. Additionally, the other biosensors for detecting microorganisms reported in recent studies were also introduced in this review.

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“…Researchers were designed three unique systems of gene circuits in Pseudomonas aeruginosa (Yang et al (2020). The first system was combined with the green fluorescent protein (GFP) gene that can give a proper visual response, the second system was uncombined with the GFP gene and the third was semicombined with the GFP gene and they found wider detection range for mercury (10 nM -7.5 mM) (Du et al, 2019).…”

Section: Whole-cell Biosensorsmentioning

confidence: 99%

Modern nanobiotechnologies for efficient detection and remediation of mercury

Gaur

Yadav

Kushwah

et al. 2021

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Purpose This information will be useful in the selection of materials and technology for the detection and removal of mercury ions at a low cost and with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. Design/methodology/approach Different nano- and bio-materials allowed for the development of a variety of biosensors – colorimetric, chemiluminescent, electrochemical, whole-cell and aptasensors – are described. The materials used for their development also make it possible to use them in removing heavy metals, which are toxic contaminants, from environmental water samples. Findings This review focuses on different technologies, tools and materials for mercury (heavy metals) detection and remediation to environmental samples. Originality/value This review gives up-to-date and systemic information on modern nanotechnology methods for heavy metal detection. Different recognition molecules and nanomaterials have been discussed for remediation to water samples. The present review may provide valuable information to researchers regarding novel mercury ions detection sensors and encourage them for further research/development.

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Feedback regulation mode of gene circuits directly affects the detection range and sensitivity of lead and mercury microbial biosensors

Cited by 29 publications

References 29 publications

Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor

Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor

Transcription Factor-Based Biosensors for Detecting Pathogens

Modern nanobiotechnologies for efficient detection and remediation of mercury

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