Feed Fermentation with Reuteran- and Levan-Producing Lactobacillus reuteri Reduces Colonization of Weanling Pigs by Enterotoxigenic Escherichia coli

Yang, Yan; Galle, Sandra; Le, Minh Hong Anh; Zijlstra, R. T.; Gänzle, Michael G.

doi:10.1128/aem.01525-15

Cited by 55 publications

(44 citation statements)

References 53 publications

(65 reference statements)

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“…Protection against ETEC K88 adhesion and membrane damage Roselli et al, 2007 Bifidobacterium No development of diarrhea -all pigs remained healthy Yang et al, 2015b …”

Section: Piglet Intestinementioning

confidence: 99%

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

Roselli

Pieper

Rogel–Gaillard

et al. 2017

Animal Feed Science and Technology

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms HIGHLIGHTS-Updated literature overview about probiotics, prebiotics and synbiotics on pig gut health is provided -Both in vivo studies performed in pigs and in vitro studies conducted in pig intestinal cell lines are described -A critical outcome of the described results is provided -The concept of "proxy measurements" for pig gut health is widely discussed -The link between gut microbiota and mucsal immune system is described Abstract:Probiotics are live microorganisms that can confer a health benefit on the host, and amongst various mechanisms probiotics are believed to exert their effects by production of antimicrobial substances, competition with pathogens for adhesion sites and nutrients, enhancement of mucosal barrier integrity and immune modulation. Through these activities probiotics can support three core benefits for the host: supporting a healthy gut microbiota, a healthy digestive tract and a healthy immune system. More recently, the concept of combining probiotics and prebiotics, i.e. synbiotics, for the beneficial effect on gut health of pigs has attracted major interest, and examples of probiotic and prebiotic benefits for pigs are pathogen inhibition and immunomodulation. Yet, it remains to be defined in pigs, what exactly is a healthy gut. Because of the high level of variability in growth and feed conversion between individual pigs in commercial production systems, measuring the impact of probiotics on gut health defined by improvements in overall productivity requires large experiments. For this reason, many studies have concentrated on measuring the effects of the feed additives on proxies of gut health including many immunological measures, in more controlled experiments. With the major focus of studying the balance between gut microbiology, immunology and physiology, and the potential for prevention of intestinal disorders in pigs, we therefore performed a literature review of the immunomodulatory effects of probiotics, either alone or in combination with prebiotics, based on in vivo, in vitro and ex vivo porcine experiments. A 3 consistent number of studies showed the potential capacity in terms of immunomodulatory activities of these feed additives in pigs, but contrasting results can also be obtained from the literature.Reasons for this are not clear but could be related to differences with respect to the probiotic strain used, experimental settings, diets, initial microbiota colonization, administration route, time and frequency of administration of the probiotic strain and sampling for analysis. Hence, the use of proxy measurements of enteric health based on observable immunological parameters presents significant problems at the moment, and cannot be considered robust, reliable predictors of the probiotic activity in vivo, in relation to pig gut health. In c...

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“…Protection against ETEC K88 adhesion and membrane damage Roselli et al, 2007 Bifidobacterium No development of diarrhea -all pigs remained healthy Yang et al, 2015b …”

Section: Piglet Intestinementioning

confidence: 99%

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

Roselli

Pieper

Rogel–Gaillard

et al. 2017

Animal Feed Science and Technology

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“…Feed fermentations were performed as previously described (Yang et al . ). To confirm the identity of the inoculum with fermentation microbiota, the pH, the viable cell counts and the colony morphology of isolates from of each batch of fermented feed were monitored.…”

Section: Methodsmentioning

confidence: 97%

“…Lactobacillus reuteri TMW1.656 is a rodent‐lineage representative of the vertebrate‐host adapted species L. reuteri with documented probiotic activity in swine (Yang et al . ); L. casei has been attributed a nomadic lifestyle and L. fermentum is associated with environmental habitats (Duar et al . ).…”

Section: Methodsmentioning

confidence: 99%

Impact of probiotic Lactobacillus sp. on autochthonous lactobacilli in weaned piglets

et al. 2018

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Aims: This study aimed to determine whether host-adapted lactobacilli exhibit superior survival during intestinal transit relative to nomadic and free-living organisms, and to characterize the impact of probiotic lactobacilli on autochthonous lactobacilli. Methods and Results: Mixed cultures of Lactobacillus casei K9-1 and Lactobacillus fermentum K9-2, or reutericyclin producing Lactobacillus reuteri and its isogenic mutant were fed to piglets as freeze-dried culture, or as part of fermented feed. Lactobacilli in digesta and faecal samples were quantified by strain-specific quantitative PCR (qPCR), high-resolution-melting curve qPCR, and high-throughput sequencing of 16S rRNA gene sequence tags. The abundance of the host adapted L. reuteri in digesta and faeces was higher (P < 0Á05) when compared to L. casei or L. fermentum. Feed fermentation or chemical acidification of feed reduced (P < 0Á05) cell counts of Lactobacillus salivarius in colonic digesta. The reutericyclin producing L. reuteri TMW1.656 transiently reduced (P < 0Á05) the faecal abundance of lactobacilli. However, the overall impact of probiotic intervention on autochthonous lactobacilli was minor. Conclusions: The vertebrate host-adapted L. reuteri survives better during intestinal transit of piglets compared to L. casei and L. fermentum. Significance and Impact of the Study: Ecology and lifestyle of Lactobacillus strains may be suitable criteria for selection of probiotic strains for use in swine production.

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“…The effects of feed fermentation with L. reuteri TMWI.656 on growth performance and the abundance of ETEC in weaning piglets was studied [75]. L. reuteri strains produce reuteran or levan exopolysaccharides that inhibit ETEC adherence to the mucosa.…”

Section: Probiotic Activitiesmentioning

confidence: 99%

Enterotoxigenic <i>Escherichia coli</i> and probiotics in swine: what the bleep do we know?

Dubreuil

2017

Bioscience of Microbiota, Food and Health

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The concept of certain microorganisms conferring direct benefits to the host relates to the term “probiotic”. Probiotics are microorganisms, bacteria, or yeast that when administered orally in sufficient quantity can counteract the effect of pathogenic microorganisms. The gastrointestinal (GI) tract is the site where probiotics are believed to play the most important role. The proposed effects of probiotics include antagonism of pathogens, interference with adherence, competition for nutrients, enterotoxin inactivation, modulation of the immune response, and strengthening of the intestinal barrier. From birth to postweaning, piglets are very sensitive to gut colonisation by pathogens. Enterotoxigenic Escherichia coli represents one of the most common agents of swine diarrhoea. The enterotoxins produced by this E. coli virotype are responsible for the loss of electrolytes and water observed following infection. This review addresses more specifically the studies done during the last 10 years deciphering the molecular mechanisms at play between host cell and probiotic interactions in the swine GI tract.

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Feed Fermentation with Reuteran- and Levan-Producing Lactobacillus reuteri Reduces Colonization of Weanling Pigs by Enterotoxigenic Escherichia coli

Cited by 55 publications

References 53 publications

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

Immunomodulating effects of probiotics for microbiota modulation, gut health and disease resistance in pigs

Impact of probiotic Lactobacillus sp. on autochthonous lactobacilli in weaned piglets

Enterotoxigenic <i>Escherichia coli</i> and probiotics in swine: what the bleep do we know?

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