Feed-based bivalent vaccine upregulates expressions of immune-related genes in systemic and mucosal tissues of red hybrid tilapia (Oreochromis niloticus × O. mossambicus) against Streptococcus iniae and Aeromonas hydrophila

Monir, Md. Shirajum; Yusoff, Sabri Mohd; Zulperi, Zarirah; Hassim, Hasliza Abu; Zamri‐Saad, Mohd; Amal, Mohammad Noor Azmai; Salleh, Annas; Mohamad, Aslah; Azzam-Sayuti, Mohamad; Yasin, Ina Salwany Md

doi:10.1007/s10499-022-00925-4

Cited by 2 publications

(2 citation statements)

References 59 publications

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“…The MHC‐II, CD4 and IL‐1β transcript levels were significantly higher in the vaccinated fish hindgut than the control fish hindgut ( p ≤ .05), as confirmed using the RT‐qPCR assay. Similarly, the up‐regulation of MHC‐II (11.7‐fold), CD4 (8.1‐fold) and IL‐1β (10.9‐fold) was also reported in red hybrid tilapia vaccinated with an oral vaccine containing killed whole organisms of S. iniae and A. hydrophila (Monir et al., 2022). Results suggested that the FBBV pellet successfully stimulate innate and adaptive immunological responses in red hybrid tilapia as indicated by immune‐related gene expression following vaccination.…”

Section: Discussionmentioning

confidence: 63%

“…To evaluate the immune‐related gene expression pattern throughout the 8‐week experimental period, the hindgut samples (50 mg) were subjected to total RNA extraction based on the previous study (Monir et al., 2022). The extracted total RNA sample (1 μg) was reverse transcribed using a OneScript® Hot cDNA Synthesis kit (Applied Biological Materials Inc., Canada) in order to produce cDNA.…”

Section: Methodsmentioning

confidence: 99%

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Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Ali,

Ngalimat,

Saad

et al. 2024

Journal of Fish Diseases

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Streptococcosis and aeromoniasis are the main obstacles to sustainable tilapia production. Vaccination offered an effective method to control microbial infections. Previously, a feed‐based bivalent vaccine (FBBV) containing killed whole organisms of Streptococcus agalactiae and Aeromonas hydrophila mixed with 10% palm oil was successfully developed, which provided good protection against streptococcosis and aeromoniasis in Oreochromis sp. However, the mechanisms of immunities in vaccinated fish still need clarification. Here, the hindgut transcriptome of vaccinated and control fish was determined, as the gut displays higher affinity towards antigen uptake and nutrient absorption. The efficacy of FBBV to improve fish immunity was evaluated according to the expression of immune‐related genes in the vaccinated fish hindgut throughout the 8‐week experimental period using RT‐qPCR. The vaccinated fish hindgut at week 6 was further subjected to transcriptomic analysis due to the high expression of immune‐related genes and contained killed whole organisms. Results demonstrated the expression of immune‐related genes was in correlation with the presence of killed whole organisms in the vaccinated fish hindgut. Transcriptomic analysis has allowed the prediction of robust immune‐related pathways, including innate and adaptive immunological responses in vaccinated fish hindgut than control fish. Pathways related to the regulation of lipid metabolism and modulation of the immune system were also significantly enriched (p ≤ .05). Overall, results offer a fundamental study on understanding the immunological response in Oreochromis sp. following vaccination with the FBBV pellet and support further application to prevent bacterial diseases in aquaculture.

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Section: Discussionmentioning

confidence: 63%

Section: Methodsmentioning

confidence: 99%

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Ali,

Ngalimat,

Saad

et al. 2024

Journal of Fish Diseases

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Efficacy of combined inactivated vaccines against Vibrio alginolyticus and Streptococcus agalactiae infections in Nile tilapia in Egypt

et al. 2023

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Vibrio alginolyticus and Streptococcus agalactiae are important bacterial pathogens that yielded high losses in Nile tilapia in Egypt. The present study aimed to check the protective efficacy of inactivated whole-cell bivalent vaccines against these pathogens using incomplete Freund’s adjuvant and Montanide™ IMS 1312 VG as adjuvants. The antibody titers have been determined at different weeks post-vaccination (WPV). Moreover, the protection levels against the challenged bacterial pathogens have also been examined in relation to the time-dependent protection at different WPV. The results revealed that serum antibodies were generated in all immunized fish at 1st WPV, peaked at 4th WPV, continued, and gradually decreased from 6th WPV to 14th WPV in all vaccinated groups. In addition, vaccines induced significantly higher protection of the immunized tilapia, manifested by higher survival rates. We noticed that the antibody levels and survival rates of the vaccinated fish by a vaccine adjuvanted by Montanide™ IMS 1312 VG were higher than those produced by a vaccine adjuvanted by incomplete Freund’s adjuvant at different time points. Moreover, no external clinical signs, visceral adhesions, or internal lesions were recorded in the vaccinated tilapia, demonstrating the safety of the formulated vaccines. According to the aforementioned findings, we could suggest that the prepared bivalent vaccines, using the two adjuvant types, are safe and highly protective and could be utilized as promising candidate vaccines to increase the resistance of Nile tilapia against V. alginolyticus and S. agalactiae infections. Moreover, Montanide™ IMS 1312 VG enhanced the immuno-protectivity and exhibited optimum immune response and earlier protection compared to the vaccine adjuvanted by incomplete Freund’s adjuvant, demonstrating its added value during the preparation of tilapia vaccines.

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Feed-based bivalent vaccine upregulates expressions of immune-related genes in systemic and mucosal tissues of red hybrid tilapia (Oreochromis niloticus × O. mossambicus) against Streptococcus iniae and Aeromonas hydrophila

Cited by 2 publications

References 59 publications

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Expression of immuno‐transcriptome response in red hybrid tilapia (Oreochromis sp.) hindgut following vaccination with feed‐based bivalent vaccine

Efficacy of combined inactivated vaccines against Vibrio alginolyticus and Streptococcus agalactiae infections in Nile tilapia in Egypt

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