Fed batch production of hydrogen from palm oil mill effluent using anaerobic microflora

Abstract

Anaerobic production of hydrogen from palm oil mill effluent (POME) by microflora was investigated in 5-l bioreactor at 60 °C and pH 5.5. POME sludge was collected from the anaerobic pond of a POME treatment plant at a palm oil mill and used as a source of inocula. A batch reactor was found to yield a total of 4708 ml H2H2/(l POME) and the maximum evolution rate was 454 ml-H2H2/(l POME h). A fed batch process was conducted after 50 h. Two liters of reaction medium was removed and 2 l of fresh POME was added to the reactor every 24 h (15 times). The reproducibility of the fed batch process checked by changing the feeding time every 8 h (10 times). A yield of 2382 ml H2H2/(l POME) and 2419 ml H2H2/(l POME) at maximum evolution rate of 313 ml H2H2/(l POME h) and 436 ml H2H2/(l POME h) were obtained, respectively. Throughout the study, methane gas was not observed in the evolved gas mixture.

Statistical Optimization of Biohydrogen Production from Palm Oil Mill Effluent by Natural Microflora

“…The difference of the hydrogen yield obtained in this study and that of Atif et al [20] was because of the different temperatures used during the fermentative hydrogen production. The optimal substrate concentrations obtained by Mu et al [4] were about 25 g/L sucrose.…”

“…The optimal substrate concentration obtained in this study was 80 g/L POME, while the optimal substrate concentration obtained by Atif et al [20] was 70 g/L POME. The difference of the hydrogen yield obtained in this study and that of Atif et al [20] was because of the different temperatures used during the fermentative hydrogen production.…”

“…(5) could appropriately describe the effects of pH and substrate concentration on the hydrogen yield obtained in this study. ANOVA of the adjusted model ( Table 5) also showed that the linear effects of pH (A), substrate concentration (B) and quadratic effect of pH (A 2 ) were the significant terms for hydrogen production, which means these terms had great impacts on hydrogen yield [20].…”

Bioenergy from anaerobic degradation of lipids in palm oil mill effluent

“…However, various researchers reported that anaerobic microflora found in POME was able to produce hydrogen (Chong et al 2009a, b, c) and methane gas was not observed in the evolved gas (Morimoto et al 2004;Atif et al 2005). …”

Hydrogen Fermentation of Cow Manure Mixed with Food Waste

“…Hydrogen is a clean energy carrier which produces only electricity and water when it is used with fuel cells. Anaerobic digestion to produce hydrogen, known as hydrogen fermentation, has been studied using various organic wastes, such as sugary wastewater 11 , bean curd manufacturing waste 5 , a wheat starch coproduct 3 , palm oil mill effluent 1 , food wastes 7 , and cow manure 14,15 . Wastewater treated by hydrogen fermentation can be re-used as feedstock for methane fermentation, because volatile fatty acids (VFAs) produced by hydrogen fermentation serve as substrates for methane production.…”