Fecundity in adult Haemonchus contortus parasites is correlated with abomasal tissue eosinophils and γδ T cells in resistant Canaria Hair Breed sheep

González, J.; Hernández, A. M.; Meeusen, Elza Nicole Theresia; Rodrı́guez, Francisco; Molina, José Molina; Jaber, José Raduán; Raadsma, Herman W.; Piedrafita, David

doi:10.1016/j.vetpar.2011.01.005

Cited by 38 publications

(24 citation statements)

References 28 publications

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“…The two groups of goats have showed distinct differences in GIN resistance (see Table 1 and Figure 1), even when we allowed them to co-graze together on the same pasture under natural conditions [25]. Under artificial challenge trial with Haemonchus contortus , we observed that resistant goats had lower FECs than the susceptible goats, by approximately 50%, similar to previous studies [25,26]. Additionally, resistant YWGs not only shed fewer GIN eggs, but also tended to have a delayed egg production, indicating an anti-fecundity effect of the immune response in this selected group.…”

Section: Discussionsupporting

confidence: 87%

Exploring the Genetic Resistance to Gastrointestinal Nematodes Infection in Goat Using RNA-Sequencing

Bhuiyan

et al. 2017

IJMS

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Gastrointestinal nematodes (GINs) are one of the most economically important parasites of small ruminants and a major animal health concern in many regions of the world. However, the molecular mechanisms of the host response to GIN infections in goat are still little known. In this study, two genetically distinct goat populations, one relatively resistant and the other susceptible to GIN infections, were identified in Yichang goat and then four individuals in each group were chosen to compare mRNA expression profiles using RNA-seq. Field experiment showed lower worm burden, delayed and reduced egg production in the relatively resistant group than the susceptible group. The analysis of RNA-seq showed that 2369 genes, 1407 of which were up-regulated and 962 down-regulated, were significantly (p < 0.001) differentially expressed between these two groups. Functional annotation of the 298 genes more highly expressed in the resistant group yielded a total of 46 significant (p < 0.05) functional annotation clusters including 31 genes (9 in innate immunity, 13 in immunity, and 9 in innate immune response) related to immune biosynthetic process as well as transforming growth factor (TGF)-β, mitogen-activated protein kinase (MAPK), and cell adhesion molecules (CAMs) pathways. Our findings provide insights that are immediately relevant for the improvement of host resistance to GIN infections and which will make it possible to know the mechanisms underlying the resistance of goats to GIN infections.

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Section: Discussionsupporting

confidence: 87%

Exploring the Genetic Resistance to Gastrointestinal Nematodes Infection in Goat Using RNA-Sequencing

Bhuiyan

et al. 2017

IJMS

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“…The CHB sheep used in this study have been shown to have enhanced resistance to H contortus after a single infection, with resistance directed against the adult parasite stage manifested in reduced worm length and fecundity . Further studies have shown that this resistance correlates significantly with both δ–T cells and eosinophils and that depletion of δ–T cells abrogates protection as well as the eosinophil correlation . The present study shows a similar effect on adult worms after targeting eosinophilia through recombinant IL‐5 immunization and supports the initial hypothesis that both δ–T cells and eosinophils, possibly in cooperation, are responsible for the effect on adult worms in the resistant CHB sheep.…”

Section: Discussionsupporting

confidence: 82%

“…In immunized animals, a negative association between eosinophils and faecal egg count (FEC) has been demonstrated and eosinophils are observed surrounding the H contortus larvae in abomasal tissues . In a resistant breed of sheep, the Canaria Hair Breed (CHB) sheep, immunity manifests after a single infection with H contortus and eosinophil numbers have been correlated with reduction in adult worm burden and fecundity . These data suggest an important role for eosinophils in immune mechanisms against both larvae and adult H contortus , but do not provide direct evidence for their involvement in immune protection.…”

Section: Introductionmentioning

confidence: 99%

Increased susceptibility to Haemonchus contortus infection by interleukin‐5 modulation of eosinophil responses in sheep

Hernández

Meeusen

Rodrı́guez

et al. 2019

Parasite Immunology

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Eosinophils are prominent effector cells in immune responses against gastrointestinal nematode infections in ruminants, but their in vivo role has been hard to establish in large animals. Interleukin‐5 is a key cytokine in the induction and stimulation of anti‐parasitic eosinophil responses. This study attempted to modulate the eosinophil response in sheep through vaccination with recombinant interleukin‐5 (rIL‐5) and determine the effect on subsequent Haemonchus contortus infection. Nematode‐resistant Canaria Hair Breed (CHB) sheep vaccinated with rIL‐5 in Quil‐A adjuvant, had lower blood eosinophil counts and higher mean worm burdens than control sheep vaccinated with Quil‐A adjuvant alone. In addition, adult worms in IL‐5‐vaccinated sheep were significantly longer with higher eggs in utero in female worms, supporting an active role of eosinophils against adult parasites in CHB sheep. These results confirm that eosinophils can play a direct role in effective control of H contortus infection in sheep and offer a new approach to study immune responses in ruminants.

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“…Although the mean dose of L 3 larvae was almost the same, approximately 239 and 265 larvae per kilogram of initial body weight for resistant and susceptible goat respectively. Susceptible goats showed approximately 60% higher FEC compared to that of resistant goats that were similar to previous finding (González et al 2011). Additionally, the challenge trial also showed that resistant YWG had a tendency to delayed egg production and shed fewer eggs at day 28, 35, and 42 of post-infection.…”

Section: Discussionsupporting

confidence: 87%

Assessment of genetic resistance and abomasal tissues expression of Yichang white goat experimentally challenge with Haemonchus contortus infection

et al. 2019

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Our previous association study revealed the mutation in candidate immune genes (NOD1 & NLRP9) was significantly associated with FEC of Haemonchus contortus infection in Yichang white goats, but the relative expression of mRNA of those genes associated with resistance to H. contortus was not investigated. Aim of the current experiment was to evaluate the susceptible and resistant individuals to nematode infection within the population of Yichang white goat (YWG) and assess the differential level of mRNA expression of those candidate genes in the abomasal tissues of susceptible and resistant goats. Fecal egg count (FEC) was determined using a modified McMaster technique, and the hematological parameter was measured by Mindray auto hematology analyzer. Phenotype data were collected and analyzed using a generalized linear model with SAS statistical program. Field investigate revealed that the prevalence (76%) with maxium parasite load (734.34±84.21epg) of H. contortus occurred in August within the experimental flock FEC in resistant group (103.38±1.20epg) and susceptible group (1180.25±43.53epg) group demonstrated the presence of two distinct goat populations within this breed. Four resistant and four susceptible goats were selected from each group. The parasite infection was established by artificially challenge with 5000 infective L3 larvae of H. contortus. Abomasal tissues were collected from all experimental goats after 42 days of post-infection. FEC, Body weight, packed cell volume, and hemoglobin value were significantly different (P < 0.01) between resistant and susceptible group of goats. Quantitative real-time PCR in abomasal tissues revealed that the expression level of mRNA for NOD1 (P < 0.00001), IFNG (P < 0.0001), NLRP9, TLR8, IL32, (P < 0.001) and IGF1 (P < 0.01) was higher in resistant goat compared to susceptible, except SFTPA1. These findings revealed the presence of genetic resistant individuals to H. contortus within the goat breed and expression of NOD1 and NLRP9 genes proved the positive finding of our previous study. Presence of genetic resistant individuals in Yichang white goat YWG breed could be a good candidate for selective breeding and highly expressed genes related to resistant could be used as biomarkers to develop H. contortus resistant goat population. Progressive Agriculture 30 (2): 194-208, 2019

show abstract

Fecundity in adult Haemonchus contortus parasites is correlated with abomasal tissue eosinophils and γδ T cells in resistant Canaria Hair Breed sheep

Cited by 38 publications

References 28 publications

Exploring the Genetic Resistance to Gastrointestinal Nematodes Infection in Goat Using RNA-Sequencing

Exploring the Genetic Resistance to Gastrointestinal Nematodes Infection in Goat Using RNA-Sequencing

Increased susceptibility to Haemonchus contortus infection by interleukin‐5 modulation of eosinophil responses in sheep

Assessment of genetic resistance and abomasal tissues expression of Yichang white goat experimentally challenge with Haemonchus contortus infection

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