Fecal microbiota transplantation as tool to study the interrelation between microbiota composition and miRNA expression

Wortelboer, Koen; Bakker, Guido J.; Winkelmeijer, Maaike; Riel, Natal A. W. van; Levin, Evgeni; Nieuwdorp, Max; Herrema, Hilde; Davids, Mark

doi:10.1016/j.micres.2022.126972

Cited by 9 publications

(6 citation statements)

References 62 publications

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“…Gut microbiota has been shown to affect the expression of circulating miRNAs [ 105 ] and thus determine intestinal epithelial proliferation and differentiation. Results from mouse models have demonstrated that miR-156 inhibited intestinal cell proliferation by the Wnt/β-catenin signaling pathway [ 106 ] and miR-31 promoted intestinal epithelial cell proliferation through the Wnt/Hippo signaling pathway to increase epithelial regeneration following injury [ 74 ].…”

Section: The Gut Microbiota–microrna Interactions and Intestinal Immu...mentioning

confidence: 99%

Gut Microbiota–MicroRNA Interactions in Intestinal Homeostasis and Cancer Development

et al. 2022

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Pre-clinical models and clinical studies highlight the significant impact of the host–microbiota relationship on cancer development and treatment, supporting the emerging trend for a microbiota-based approach in clinical oncology. Importantly, the presence of polymorphic microbes is considered one of the hallmarks of cancer. The epigenetic regulation of gene expression by microRNAs affects crucial biological processes, including proliferation, differentiation, metabolism, and cell death. Recent evidence has documented the existence of bidirectional gut microbiota–microRNA interactions that play a critical role in intestinal homeostasis. Importantly, alterations in microRNA-modulated gene expression are known to be associated with inflammatory responses and dysbiosis in gastrointestinal disorders. In this review, we summarize the current findings about miRNA expression in the intestine and focus on specific gut microbiota–miRNA interactions linked to intestinal homeostasis, the immune system, and cancer development. We discuss the potential clinical utility of fecal miRNA profiling as a diagnostic and prognostic tool in colorectal cancer, and demonstrate how the emerging trend of gut microbiota modulation, together with the use of personalized microRNA therapeutics, might bring improvements in outcomes for patients with gastrointestinal cancer in the era of precision medicine.

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Section: The Gut Microbiota–microrna Interactions and Intestinal Immu...mentioning

confidence: 99%

Gut Microbiota–MicroRNA Interactions in Intestinal Homeostasis and Cancer Development

et al. 2022

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“…Another recent study using fecal microbiota transplantation in humans was performed with two groups, an allogenic (received feces from lean healthy donor) and an autologous (control = received own feces). This study observed a significant correlation between the fecal miRNA expression and microbiota composition for both groups, evidencing an involvement of the gut microbiota in regulating intestinal miRNA expression [ 30 ].…”

Section: Interaction Between Micrornas and Microbiota In Inflammatory...mentioning

confidence: 99%

“…Recently, the role of miRNAs and their interactions with the host and its microbiota have been gaining prominence and becoming the target of several studies that demonstrate the participation of miRNAs in the modulation of the intestinal microbiota and induction of dysbiosis, whereas the intestinal microbiota, in turn, can regulate the expression of miRNAs and consequently alter intestinal homeostasis [ 29 , 30 , 116 , 117 , 118 ] ( Figure 1 ).…”

Section: Interaction Between Micrornas and Microbiota In Inflammatory...mentioning

confidence: 99%

“…Both the intestinal microbiota and miRNAs have been the target of recent studies aimed at gaining an appreciation of their roles and relationship with IBD [ 29 , 30 , 31 , 32 ]. Considering that intestinal microbiota and miRNAs are both strongly related to IBD, understanding the role of each in the IBD process can provide vital information that could result in the development of more effective and accurate diagnostic tools and target treatments for patients with IBD.…”

Section: Introductionmentioning

confidence: 99%

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Intestinal Microbiota and miRNA in IBD: A Narrative Review about Discoveries and Perspectives for the Future

Oliveira

Quaglio

Magro

et al. 2023

IJMS

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Inflammatory bowel disease (IBD) includes Crohn’s disease (CD) and ulcerative colitis (UC) and comprises a chronic gastrointestinal tract disorder characterized by hyperactive and dysregulated immune responses to environmental factors, including gut microbiota and dietary components. An imbalance of the intestinal microbiota may contribute to the development and/or worsening of the inflammatory process. MicroRNAs (miRNAs) have been associated with various physiological processes, such as cell development and proliferation, apoptosis, and cancer. In addition, they play an important role in inflammatory processes, acting in the regulation of pro- and anti-inflammatory pathways. Differences in the profiles of miRNAs may represent a useful tool in the diagnosis of UC and CD and as a prognostic marker in both diseases. The relationship between miRNAs and the intestinal microbiota is not completely elucidated, but recently this topic has gained prominence and has become the target of several studies that demonstrate the role of miRNAs in the modulation of the intestinal microbiota and induction of dysbiosis; the microbiota, in turn, can regulate the expression of miRNAs and, consequently, alter the intestinal homeostasis. Therefore, this review aims to describe the interaction between the intestinal microbiota and miRNAs in IBD, recent discoveries, and perspectives for the future.

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“…Fecal microbiota transplantation (FMT) is an effective method for reestablishing the gut microbiota by transplanting the intestinal microbial community of a healthy donor into the gastrointestinal tract of the recipient, thereby rapidly changing the recipient's gut microbiota composition [15]. An oral glucose tolerance test (OGTT) showed that FMT significantly decreased blood glucose and glycated hemoglobin (HbA1c) levels, improved insulin resistance, and increased insulin sensitivity in T2DM mice [16].…”

Section: Introductionmentioning

confidence: 99%

Phellinus igniarius Polysaccharides Ameliorate Hyperglycemia by Modulating the Composition of the Gut Microbiota and Their Metabolites in Diabetic Mice

Ni,

Li,

Qian

et al. 2023

Molecules

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Gut microbiota dysbiosis has been reported as a risk factor in the development of type 2 diabetes mellitus (T2DM). Polysaccharides from Phellinus igniarius (P. igniarius) possess various properties that help manage metabolic diseases; however, their underlying mechanism of action remains unclear. Therefore, in this study, we aimed to evaluate the effect of P. igniarius polysaccharides (SH-P) on improving hyperglycemia in mice with T2DM and clarified its association with the modulation of gut microbiota and their metabolites using 16S rDNA sequencing and liquid chromatography–mass spectrometry. Fecal microbiota transplantation (FMT) was used to verify the therapeutic effects of microbial remodeling. SH-P supplementation alleviated hyperglycemia symptoms in T2DM mice, ameliorated gut dysbiosis, and significantly increased the abundance of Lactobacillus in the gut. Pathway enrichment analysis indicated that SH-P treatment altered metabolic pathways associated with the occurrence and development of diabetes. Spearman’s correlation analysis revealed that changes in the dominant bacterial genera were significantly correlated with metabolite levels closely associated with hyperglycemia. Additionally, FMT significantly improved insulin sensitivity and antioxidative capacity and reduced inflammation and tissue injuries, indicating improved glucose homeostasis. These results indicate that the ameliorative effects of SH-P on hyperglycemia are associated with the modulation of gut microbiota composition and its metabolites.

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Fecal microbiota transplantation as tool to study the interrelation between microbiota composition and miRNA expression

Cited by 9 publications

References 62 publications

Gut Microbiota–MicroRNA Interactions in Intestinal Homeostasis and Cancer Development

Gut Microbiota–MicroRNA Interactions in Intestinal Homeostasis and Cancer Development

Intestinal Microbiota and miRNA in IBD: A Narrative Review about Discoveries and Perspectives for the Future

Phellinus igniarius Polysaccharides Ameliorate Hyperglycemia by Modulating the Composition of the Gut Microbiota and Their Metabolites in Diabetic Mice

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