Fecal metabonomics combined with 16S rRNA gene sequencing to analyze the changes of gut microbiota in rats with kidney-yang deficiency syndrome and the intervention effect of You-gui pill

Chen, Ruiqun; Wang, Jia; Zhan, Runhua; Zhang, Lei; Wang, Xiufeng

doi:10.1016/j.jep.2019.112139

Cited by 61 publications

(42 citation statements)

References 66 publications

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“…We found that Sunitinib, Citrate, N2-Acetyl-L-ornithine, D-Alanyl-D-alanine, L-Valine, Tetrahydrocorticosterone, (S)-2-aminobutyric acid, (S)-Equol, Succinate, L-Leucine, Acetyl-DL-Leucine, Glutaric acid, Sarcosine, N-Acetyl-L-glutamate, D-Glucuronate, 3-Dehydroshikimic acid, Adenine, Kynurenic acid, L-Cysteic acid, Isobutyric acid, 1-Methylxanthine, N-Acetyl-L-glutamate, D-Glucuronate, 3-Dehydroshikimic acid, Adenine, Kynurenic acid, L-Cysteic acid, Isobutyric acid and 1-Methylxanthine correlated with body weight. Similarly, Some studies [3] also found that bacterially-produced metabolites such as valine, isoleucine, acetate, taurine, glycine, glycerol, and tryptophan, are involved in amino acid metabolism, energy metabolism, especially endocrine, lipid metabolism, and carbohydrate metabolism.…”

Section: Different Metabolites Might Affect the Weight Changes Of Offspringmentioning

confidence: 93%

Effects of S24–7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

et al. 2021

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Antibiotic exposure during pregnancy will adversely affect the growth of offspring; however, this remains controversial and the mechanism is poorly understood. To study this phenomenon, we added ceftriaxone sodium to the drinking water of pregnant rats and continuously monitored the body weight of their offspring. The results showed that compared with the control group, the offspring exposed to antibiotics during pregnancy had a higher body weight up to 3 weeks old but had a lower body weight at 6 weeks old. To determine the role of the gut microbiota and its metabolites in the growth of offspring, we collected feces for sequencing and further established that the experimental group has a different composition ratio of dominant bacteria at 6 week old, among which S24–7 correlated negatively with body weight and the metabolites that correlated with body weight-related unique flora were L-Valine, L-Leucine, Glutaric acid, N-Acetyl-L-glutamate, and 5-Methylcytosine. To further explore how they affect the growth of offspring, we submitted these data to Kyoto Encyclopedia of Genes and Genomes website for relevant pathway analysis. The results showed that compared with the control, the following metabolic pathways changed significantly: Valine, leucine, and isoleucine biosynthesis; Protein digestion and absorption; and Mineral absorption. Therefore, we believe that our findings support the conclusion that ceftriaxone sodium exposure in pregnancy has a long-lasting adverse effect on the growth of offspring because of an imbalance of gut microbiota, especially S24–7, via different metabolic pathways.

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Section: Different Metabolites Might Affect the Weight Changes Of Offspringmentioning

confidence: 93%

Effects of S24–7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

et al. 2021

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“…The supernatant was discarded, and the precipitate was placed in liquid nitrogen for 1 h and stored in a refrigerator at À 80°C. [37] Preparation of Fecal DNA for High-Throughput Sequencing Analysis…”

Section: Preparation Of Isolated Intestinal Flora Of Spleen Deficiencmentioning

confidence: 99%

In Vitro Effects of Ginseng and the Seed of Zizyphus jujuba var. spinosa on Gut Microbiota of Rats with Spleen Deficiency

Yang

Song

et al. 2020

Chemistry & Biodiversity

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Ginseng and the seed of Zizyphus jujuba var. spinosa, which are traditional Chinese medicinal materials, were often used in ancient Chinese recipes as a pair of medicines. They can replenish the primordial qi and tonify the spleen. This study investigated the effects of ginseng and the seed of Zizyphus jujuba var. spinosa (GS) extract on gut microbiota diversity in rats with spleen deficiency syndrome (SDS). A total of 52 compounds (including 16 flavonoids, 35 saponins, and 1 alkaloid) were identified and analyzed from the GS extract by UPLC‐Q‐Orbitrap‐MS/MS. The GS extract significantly increased the relative abundance of Firmicutes and Bacteroidetes in rats with SDS but decreased that of Proteobacteria and Actinobacteria. At the genus level, the GS extract significantly increased the relative abundance of Lactobacillus and Bifidobacterium in rats with SDS but decreased that of Streptococcus, Escherichia‐Shigella, Veillonella, and Enterococcus. In addition, the GS extract influenced glucose and amino acid metabolism. In summary, the results showed that the GS extract changed the structure and diversity of gut microbiota in rats with SDS and balanced the metabolic process.

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“…We found that Sunitinib, Citrate, N2-Acetyl-L-ornithine, D-Alanyl-D-alanine, L-Valine, Tetrahydrocorticosterone, (S)-2-aminobutyric acid, (S)-Equol, Succinate, L-Leucine, Acetyl-DL-Leucine, Glutaric acid, Sarcosine, N-Acetyl-L-glutamate, D-Glucuronate, 3-Dehydroshikimic acid, Adenine, Kynurenic acid, L-Cysteic acid, Isobutyric acid, 1-Methylxanthine, N-Acetyl-L-glutamate, D-Glucuronate, 3-Dehydroshikimic acid, Adenine, Kynurenic acid, L-Cysteic acid, Isobutyric acid and 1-Methylxanthine correlated with body weight. Similarly, Some studies (Chen et al, 2019) also found that bacterially-produced metabolites such as valine, isoleucine, acetate, taurine, glycine, glycerol, and tryptophan, are involved in amino acid metabolism, energy metabolism, especially endocrine, lipid metabolism, and carbohydrate metabolism.…”

Section: Different Metabolites Might Affect the Weight Changes Of Offspringmentioning

confidence: 86%

Effects of S24-7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

Yang

Tang

Wen

et al. 2021

Preprint

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Antibiotic exposure during pregnancy will adversely affect the growth of offspring; however, this remains controversial and the mechanism is poorly understood. To study this phenomenon, we added ceftriaxone sodium to the drinking water of pregnant rats and continuously monitored the body weight of their offspring. The results showed that compared with the control group, the offspring exposed to antibiotics during pregnancy had a higher body weight up to 3 weeks old but had a lower body weight at 6 weeks old. To determine the role of the gut microbiota and its metabolites in the growth of offspring, we collected feces for sequencing and further established that the experimental group has a different composition ratio of dominant bacteria at 6 week old, among which S24-7 correlated negatively with body weight and the metabolites that correlated with body weight-related unique flora were L-Valine, L-Leucine, Glutaric acid, N-Acetyl-L-glutamate, and 5-Methylcytosine. To further explore how they affect the growth of offspring, we submitted these data to Kyoto Encyclopedia of Genes and Genomes website for relevant pathway analysis. The results showed that compared with the control, the following metabolic pathways changed significantly: Valine, leucine, and isoleucine biosynthesis; Protein digestion and absorption; and Mineral absorption. Therefore, we believe that our findings support the conclusion that ceftriaxone sodium exposure in pregnancy has a long-lasting adverse effect on the growth of offspring because of an imbalance of gut microbiota, especially S24-7, via different metabolic pathways.

show abstract

Fecal metabonomics combined with 16S rRNA gene sequencing to analyze the changes of gut microbiota in rats with kidney-yang deficiency syndrome and the intervention effect of You-gui pill

Cited by 61 publications

References 66 publications

Effects of S24–7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

Effects of S24–7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

In Vitro Effects of Ginseng and the Seed of Zizyphus jujuba var. spinosa on Gut Microbiota of Rats with Spleen Deficiency

Effects of S24-7 on the weight of progeny rats after exposure to ceftriaxone sodium during pregnancy

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