Fecal g. Streptococcus and g. Eubacterium_coprostanoligenes_group combined with sphingosine to modulate the serum dyslipidemia in high-fat diet mice

Wei, Wei; Jiang, Wenbo; Tian, Zhen; Wu, Huanyu; Hua, Ning; Yan, Guangcan; Zhang, Ziwei; Li, Zixiang; Dong, Feng; Sun, Yuwei; Liu, Ying; Han, Tianshu; Wang, Maoqing; Sun, Chongde

doi:10.1016/j.clnu.2021.01.031

Cited by 74 publications

(53 citation statements)

References 40 publications

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“…Low abundance of Firmicutes microbiota can increase intestinal sensitivity to inflammation [ 59 , 60 , 61 ], suggesting that taxifolin could play an anti-inflammatory role in the intestinal tract by regulating intestinal microbiota imbalance. More importantly, a large number of the top 20 genera, 8 beneficial bacteria were significantly enriched after Taxifolin treatment, and 6 of them belong to SCFA-producing bacteria, such as Ruminiclostridium_5 , Bacteroides , Lachnospiraceae_NK4A136_group , Lactobacillus , Ruminococcaceae_UCG-014 , [ Eubacterium]_coprostanoligenes_group [ 62 , 63 , 64 , 65 , 66 , 67 ]. It has been reported that SCFA-producing bacteria can benefit the host by inhibiting the expression of inflammatory factors, providing nutritional levels to colon cells, and enhancing tight junction protein expression [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

Taxifolin Alleviates DSS-Induced Ulcerative Colitis by Acting on Gut Microbiome to Produce Butyric Acid

Zhang

et al. 2022

Nutrients

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Taxifolin is a bioflavonoid which has been used to treat Inflammatory Bowel Disease. However, taxifolin on DSS-induced colitis and gut health is still unclear. Here, we studied the effect of taxifolin on DSS-induced intestinal mucositis in mice. We measured the degree of intestinal mucosal injury and inflammatory response in DSS treated mice with or without taxifolin administration and studied the changes of fecal metabolites and intestinal microflora using 16S rRNA. The mechanism was further explored by fecal microbiota transplantation. The results showed that the weight loss and diarrhea score of the mice treated with taxifolin decreased in DSS-induced mice and longer colon length was displayed after taxifolin supplementation. Meanwhile, the expression of GPR41 and GPR43 in the colon was significantly increased by taxifolin treatment. Moreover, the expression of TNF-α, IL-1β, and IL-6 in colon tissue was inhibited by taxifolin treatment. The fecal metabolism pattern changed significantly after DSS treatment, which was reversed by taxifolin treatment. Importantly, taxifolin significantly increased the levels of butyric acid and isobutyric acid in the feces of DSS-treated mice. In terms of gut flora, taxifolin reversed the changes of Akkermansia, and further decreased uncultured_bacterium_f_Muribaculaceae. Fecal transplantation from taxifolin-treated mice showed a lower diarrhea score, reduced inflammatory response in the colon, and reduced intestinal mucosal damage, which may be related to the increased level of butyric acid in fecal metabolites. In conclusion, this study provides evidence that taxifolin can ameliorate DSS-induced colitis by altering gut microbiota to increase the production of SCFAs.

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Section: Discussionmentioning

confidence: 99%

Taxifolin Alleviates DSS-Induced Ulcerative Colitis by Acting on Gut Microbiome to Produce Butyric Acid

Zhang

et al. 2022

Nutrients

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“…Feeding Lactobacillus casei NCDC 19 in high-fat diet-induced obese mice has been reported to reduce body weight gain, epididymal fat weights, blood glucose, and plasma lipids ( 34 ). In addition, g. Streptococcus and g. Eubacterium_coprostanoligenes are thought to be two hub genera in the fecal micro-ecosystem under HFD and to mediate the effect of HFD on dyslipidemia through sphingosine ( 35 ). Overall, our results suggest that WGQK intervention selectively enriches a group of gut bacteria, which might contribute to its anti-obesity and anti-hyperlipidemic effects.…”

Section: Discussionmentioning

confidence: 99%

Whole Grain Qingke Attenuates High-Fat Diet-Induced Obesity in Mice With Alterations in Gut Microbiota and Metabolite Profile

Suo

Huang

et al. 2021

Front. Nutr.

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Whole grain Qingke (WGQK) displays anti-obesity and lipid-lowering properties; however, the underlying mechanism remains elusive. This study investigated the alteration of gut microbiota composition and metabolite profile induced by WGQK intervention in mice through the integration of 16S ribosomal RNA (rRNA) sequencing and an untargeted metabolomics study. C57BL/6J male mice were fed a normal control diet (NC), high-fat diet (HFD), and HFD plus 30% WGQK (HFD+QK) for 16 weeks. The WGQK intervention decreased body weight gain, glucose tolerance, and serum lipid levels, and alleviated liver function damage induced by HFD. Moreover, WGQK changed gut microbiota composition and enriched specific genera such as Akkermansia, Bifidobacterium, and Lactobacillus. Fecal metabolomics analysis indicated that WGQK enhanced the abundance of tryptophan metabolism-related metabolites (indole, 3-indoleacetic acid, indole acetic acid (IAA), 5-hydroxyindole-3-acetic acid), histidine metabolism-related metabolites (histamine), and some unsaturated fatty acids (oleic acid, 9,10-dihydroxy-12Z-octadecenoic acid, and alpha-linolenic acid). Spearman correlation analysis revealed that these metabolites were negatively correlated with obesity-related parameters and positively correlated with the gut genera enriched by WGQK. Moreover, WGQK promoted the expression of Cholesterol 7α-hydroxylase (CYP7A1) responsible for primary bile acids production, accompanied by a decline in intestinal FXR-FGF15 expression levels. The transcript levels of two genes associated with lipogenesis, such as lipid fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) were also decreased in the HFD+QK group. Overall, our results suggest interactions between gut microbial shifts and host amino acid/lipid metabolism, and shed light on the mechanisms underlying the anti-obesity effect of WGQK.

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“…Bacteria of the genus Caproiciproducens increase caproic acid production (50), while bacteria of the genus Enterococcus enhance anticoagulation capacity (51). Meanwhile, the Eubacterium coprostanoligenes group family in P. przewalskii is correlated with the host lipid homeostasis (52). These results collectively indicate that the two Phrynocephalus lizards have had a more tolerant gut microbiome than other animals.…”

Section: Discussionmentioning

confidence: 85%

Gut Microbiome Contributes to Cold-climate Adaptation in Lizards

Chen

Zhang

Zhao

et al. 2022

Preprint

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The metabolic cold-climate adaption hypothesis predicts that animals from cold environments have relatively high metabolic rates compared with their warm-climate counterparts. However, studies testing this hypothesis are sparse. Here, we compared gut microbes between two cold-climate lizard species of the genus Phrynocephalus to test the hypothesis that gut microbiota can help lizards adapt to cold environments by promoting metabolism and absorption efficiency. We kept lizards at 24°C and 30°C for 25 d, and then collected their fecal samples to analyze and compare the microbiota based on 16S rRNA gene sequencing technology. The gut microbiota was mainly composed of Proteobacteria, Firmicutes, Bacteroidetes, and Verrucomicrobia at the phylum level in both species (Proteobacteria > Firmicutes > Verrucomicrobiota in P. erythrurus, and Bacteroidota > Proteobacteria > Firmicutes in P. przewalskii). Further analysis revealed that the gut microbiota contributed to the host’s cold adaptation, but with differences in the relative abundance of these contributory bacteria between the two species. KEGG analysis revealed that the gut microbiota primarily played roles in metabolism, genetic information processing, cellular processes, and environmental information processing in both species. Furthermore, genes related to metabolism were more abundant in P. erythrurus at 24 °C than in other species × temperature combinations, indicating the role of gut microbiota in long-term cold-climate adaptation. Our finding that gut microbiome contributes to cold-climate adaptation in both species but more evidently in P. erythrurus using colder habitats than P. przewalskii throughout a year confirms the gut microbiota’s role in the cold-climate adaptation in lizards.

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Fecal g. Streptococcus and g. Eubacterium_coprostanoligenes_group combined with sphingosine to modulate the serum dyslipidemia in high-fat diet mice

Cited by 74 publications

References 40 publications

Taxifolin Alleviates DSS-Induced Ulcerative Colitis by Acting on Gut Microbiome to Produce Butyric Acid

Taxifolin Alleviates DSS-Induced Ulcerative Colitis by Acting on Gut Microbiome to Produce Butyric Acid

Whole Grain Qingke Attenuates High-Fat Diet-Induced Obesity in Mice With Alterations in Gut Microbiota and Metabolite Profile

Gut Microbiome Contributes to Cold-climate Adaptation in Lizards

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