Fecal Calprotectin as a Useful Non-Invasive Screening Marker for Eosinophilic Gastrointestinal Disorder in Korean Children

Yoo, In Hyuk; Cho, Jin Min; Yang, Hye Ran

doi:10.3346/jkms.2020.35.e120

Cited by 14 publications

(14 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…fCal increased after infection, but levels remained within the normal limits and did not correlate with neutrophils circulating in the peripheral blood. However, interestingly, calprotectin has been shown to be expressed by eosinophils in mice( 30 ) and is elevated in eosinophilic gastrointestinal disorders( 31 ) therefore the changes in fCal levels observed in the current study could be of eosinophilic origin. We also measured HNL in feces as a specific marker for neutrophil inflammation( 32 ) and detected no increase in this marker, supporting the idea of a local, specific eosinophilic-driven response to hookworm infection.…”

Section: Discussionmentioning

confidence: 70%

Immune profiling, microbiome, metabolomics, and gut physiology of a 1-year controlled human hookworm infection

Vacca

Lavender

Noble

et al. 2023

Preprint

View full text Add to dashboard Cite

The observation that experimental helminth infection can be associated with immunomodulation and suppression of inflammatory diseases at distal tissue sites, has been used as rationale for trialing helminths such as Necator americanus for the treatment of inflammatory disorders in humans. However, the lack of sufficient knowledge of the immunological interplay between human host and parasite in a controlled infection setting limits ongoing clinical intervention studies. In this one-year longitudinal study, healthy volunteers were recruited and infected with N. americanus. Changes in immune responses, microbiome, plasma metabolome and gut physiology were examined over the course of the one-year period. All participants were successfully infected as confirmed by detectable eggs in the feces and adult worms visualized in the intestine. In general, individual variation in immune cells, serum cytokines, fecal microbiome and plasma metabolites were greater than changes induced by the infection. Nevertheless, eosinophils, serum IL-5, and fecal eosinophil degranulation markers transiently increased in the acute phase of infection. In addition, while we observed stability in microbial community composition through the course of infection, we found a difference in the microbial community composition of participants with moderate gastrointestinal symptoms. No significant changes were observed in gut physiology measured using SmartpillTM, except for a decrease in small bowel pH. Untargeted plasma metabolomics analysis of participant plasma over the course of infection revealed enrichment in tryptophan metabolism following infection which was associated with increased CTLA-4 expression on regulatory T cells (TREGS), CRTH2+ T helper 2 cells (TH2) and CCR6+ T helper 9 cells (TH9). In conclusion, hookworm infection is well tolerated and represents an innovative platform for investigating immunomodulatory properties of hookworm infection in a therapeutic clinical setting.

show abstract

Section: Discussionmentioning

confidence: 70%

Immune profiling, microbiome, metabolomics, and gut physiology of a 1-year controlled human hookworm infection

Vacca

Lavender

Noble

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Unlike the FC concentration, which showed a significant difference between the age groups, no sig-Most of the reported articles had been about IBD patients, infant diarrhea, and meta-analysis results of the IBD patient group[17] as well as review articles[18][19][20]. Studies on FC are Thus, we conducted this study on healthy male and female adults residing in Jeju, between the age of 30 and 90, to understand the normal range of FC concentration.Another notable finding in the present study's results is that FC showed a statistically significant difference between males and females.…”

mentioning

confidence: 99%

Age-Related Fecal Calprotectin Concentrations in Healthy Adults

박신영

2020

Korean J Clin Lab Sci.

View full text Add to dashboard Cite

Fecal calprotectin (FC) is a marker used for the differential diagnosis of inflammatory bowel disease (IBD). FC is also used to determine the effects of treatment and recurrence prediction because of its non-decomposition by bacteria, relative week stability at room temperature, and its uniform distribution within feces. Healthy male and female adults between the age of 30 and 80 living in Jeju were selected for this study. The FC concentration in the healthy control group (N=45) was distributed widely as 0∼545.9 μg/g and showed a significant difference with age in healthy adults. The FC concentration in adults over 70 years old (80.6 years on average) was 160.3 μg/g. The result is approximately 10 times higher than in adults below 50 years (44 years on average), with FC concentrations at 15.88 μg/g. Moreover, adults over 50 years, with an average age of 59.6, had FC concentrations of 35.46 μg/g, which were two times higher than the below 50-year-old group, confirming the significant correlation between age and FC concentration. As the FC test is a non-invasive and cost-effective objective marker in IBD tests, a suitable cutoff value is required for different ages. This study provides the baseline data for differential diagnoses.

show abstract

“…FC is a neutrophil-derived substance released during cell disruption and death in the gastrointestinal tract, and is widely used as a screening test for inflammatory bowel disease (IBD), colorectal cancer, celiac disease, and necrotizing enterocolitis of newborns. 14 15 It is also popularly used for monitoring disease activity in patients with IBD. 6 7 16 Several previous studies have proposed the potential role of FC in identifying colorectal polyps.…”

Section: Discussionmentioning

confidence: 99%

Potential Utility of Fecal Calprotectin in Discriminating Colorectal Polyps From Other Major Etiologies in Children Presenting With Isolated Hematochezia

Kim

Choi

et al. 2022

J Korean Med Sci

View full text Add to dashboard Cite

Background Colorectal polyps are the most common cause of isolated hematochezia in children, which requires a colonoscopy for diagnosis. We aimed to investigate the potential utility of fecal calprotectin (FC) in assessing colorectal polyps detected by colonoscopy among children presenting with isolated hematochezia. Methods Pediatric patients of the age of < 18 years who had undergone both colonoscopy and FC tests for isolated hematochezia from June 2016 to May 2020 were included in the present multicenter, retrospective, cross-sectional study. Comparative analysis was conducted between major causes of isolated hematochezia and FC cut-offs for discriminating colorectal polyps were explored. Results A total 127 patients were included. Thirty-five patients (27.6%) had colorectal polyps, followed by anal fissure (14.2%), ulcerative colitis (UC; 12.6%), and others. A significant difference in FC levels was observed between patients with colorectal polyps (median, 278.7 mg/kg), anal fissures (median, 42.2 mg/kg), and UC (median, 981 mg/kg) ( P < 0.001). According to receiver operating characteristic curve analysis, among patients diagnosed with colorectal polyp or anal fissure, the most accurate FC cut-off for discriminating colorectal polyps from anal fissures on colonoscopy was 225 mg/kg (sensitivity, 59.4%; specificity, 94.4%; positive predictive value [PPV], 95.0%; negative predictive value [NPV], 56.7%; area under the curve [AUC], 0.8; 95% confidence interval [CI], 0.678–0.923; P < 0.001), while among patients diagnosed with colorectal polyp or UC, the most accurate FC cut-off for discriminating colorectal polyps from UC on colonoscopy was 879 mg/kg (sensitivity, 81.2%; specificity, 56.2%; PPV, 78.8%; NPV, 60.0%; AUC, 0.687; 95% CI, 0.521–0.852; P < 0.001). Conclusion FC may assist in assessing the cause of lower gastrointestinal tract bleeding in children who present with isolated hematochezia.

show abstract

Fecal Calprotectin as a Useful Non-Invasive Screening Marker for Eosinophilic Gastrointestinal Disorder in Korean Children

Cited by 14 publications

References 24 publications

Immune profiling, microbiome, metabolomics, and gut physiology of a 1-year controlled human hookworm infection

Immune profiling, microbiome, metabolomics, and gut physiology of a 1-year controlled human hookworm infection

Age-Related Fecal Calprotectin Concentrations in Healthy Adults

Potential Utility of Fecal Calprotectin in Discriminating Colorectal Polyps From Other Major Etiologies in Children Presenting With Isolated Hematochezia

Contact Info

Product

Resources

About