Fecal Calprotectin and serum chromogranin A as potential biomarkers of irritable bowel syndrome symptom severity

Pletikosić, Sanda; Plavsic, Ivana; Hauser, Goran; Tkalčić, Mladenka

doi:10.1016/j.mehy.2015.06.008

Cited by 5 publications

(5 citation statements)

References 42 publications

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“…Clostridial species have been implicated in gastrointestinal pro‐inflammatory states including IBD 37,38 . This finding, as well as the mild increase in fecal calprotectin levels in IBS patients in our study, may indicate an inflammatory mechanism by which the microbiome affects the gut–brain axis 39 . A preliminary study from our group has shown greater symptom severity and disability with higher fecal calprotectin concentrations in children with FAPD 40 .…”

Section: Discussionsupporting

confidence: 63%

“… 37 , 38 This finding, as well as the mild increase in fecal calprotectin levels in IBS patients in our study, may indicate an inflammatory mechanism by which the microbiome affects the gut–brain axis. 39 A preliminary study from our group has shown greater symptom severity and disability with higher fecal calprotectin concentrations in children with FAPD. 40 However, in contrast to prior studies, we did not reproduce any microbial compositional associations with clinical measures, including abdominal pain.…”

Section: Discussionmentioning

confidence: 86%

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The microbiome in adolescents with irritable bowel syndrome and changes with percutaneous electrical nerve field stimulation

Castillo

Denson

Hommel

et al. 2023

Neurogastroenterology Motil

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Background Irritable bowel syndrome (IBS), a disorder of the gut–brain axis, is affected by the microbiome. Microbial studies in pediatric IBS, especially for centrally mediated treatments, are lacking. We compared the microbiome between pediatric IBS patients and healthy controls (HC), in relation to symptom severity, and with percutaneous electrical nerve field stimulation (PENFS), a non‐invasive treatment targeting central pain pathways. Methods We collected a stool sample, questionnaires and a 1–2 week stool and pain diary from 11 to 18 years patients with IBS. A patient subset completed 4 weeks of PENFS and repeated data collection immediately after and/or 3 months after treatment. Stool samples were collected from HC. Samples underwent metagenomic sequencing to evaluate diversity, composition, and abundance of species and MetaCyc pathways. Key Results We included 27 cases (15.4 ± 2.5 year) and 34 HC (14.2 ± 2.9 year). Twelve species including Firmicutes spp., and carbohydrate degradation/long‐chain fatty acid (LCFA) synthesis pathways, were increased in IBS but not statistically significantly associated with symptom severity. Seventeen participants (female) who completed PENFS showed improvements in pain (p = 0.012), disability (p = 0.007), and catastrophizing (p = 0.003). Carbohydrate degradation and LCFA synthesis pathways decreased post‐treatment and at follow‐up (FDR p‐value <0.1). Conclusions and Inferences Firmicutes, including Clostridiaceae spp., and LCFA synthesis pathways were increased in IBS patients suggesting pain‐potentiating effects. PENFS led to marked improvements in abdominal pain, functioning, and catastrophizing, while Clostridial species and LCFA microbial pathways decreased with treatment, suggesting these as potential targets for IBS centrally mediated treatments.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 86%

The microbiome in adolescents with irritable bowel syndrome and changes with percutaneous electrical nerve field stimulation

Castillo

Denson

Hommel

et al. 2023

Neurogastroenterology Motil

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“…The (37). F-calprotectin has been reported as a marker for the differentiation between IBS and inflammatory bowel diseases (16,17), and as a potential biomarker for the severity of IBS (12). Measurable F-calprotectin levels were most often noted in participants who experienced incomplete evacuation when defecating, and in participants with a higher degree of constipation.…”

Section: Discussionmentioning

confidence: 99%

“…Further investigation of AXIN1, and on its associations with several subtypes of endometriosis may allow for a deeper understanding of this disease. In addition, investigation of the potential correlation of AXIN1 expression with the expression of other readily measurable inflammatory markers, such as high-sensitivity c-reactive protein (hs-CRP) and faecal-calprotectin (F-calprotectin), may potentially offer additional diagnostic information, as these proteins have been demonstrated to be useful biomarkers in detecting inflammation (11,12). These factors may be altered in chronic inflammation and are performed as routine analyses in daily clinical practice (6).…”

Section: Introductionmentioning

confidence: 99%

Plasma AXIN1 expression exhibit negative correlations with inflammatory biomarkers and is associated with gastrointestinal symptoms in endometriosis

Dihm

Roth

et al. 2020

biom rep

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The cytoplasmic protein AXIN1 is involved in the Wnt signalling pathway and its expression is increased in patients with endometriosis compared with healthy controls. The aim of the present cross-sectional study was to further assess the levels of AXIN1 and other inflammatory biomarkers in patients with endometriosis. Patients with laparoscopy-verified endometriosis were recruited (n=172) and completed a questionnaire regarding socioeconomic factors, lifestyle habits and medical history. Plasma AXIN1 and high-sensitivity C-reactive protein (hs-CRP) levels were analysed by ELISA. The levels of calprotectin were determined in the faeces, and the haemoglobin concentration and number of erythrocytes, leukocytes and platelets were determined in the blood in a subgroup of 64 patients during clinical routine procedures. F-calprotectin expression was detected in 18 women (28.1%), who had more severe constipation and more frequently experienced incomplete evacuation when defecating, and 5 women (7.8%) exhibited elevated levels. P-AXIN1 levels were higher in patients who received hormonal treatment, and correlated inversely with faecal-calprotectin levels (P=0.003), B-haemoglobin levels (P=0.030) and the numbers of B-erythrocytes (P=0.033) and B-platelets (P=0.017), but were not correlated with hs-CRP levels (P=0.818). Higher levels of AXIN1 were associated with the duration of the gastrointestinal symptoms and with diarrhoea, constipation, vomiting and nausea and the intestinal symptoms' effect on quality of life, and tended to be associated with the duration of endometriosis. Hs-CRP expression was not associated with the clinical characteristics or symptoms of endometriosis, but higher levels were associated with obesity (P=0.002) and hormonal treatment (P=0.011). In conclusion, P-AXIN1 expression was negatively correlated with certain inflammatory biomarkers and was positively associated with gastrointestinal symptoms. P-AXIN1 levels were increased in patients who received hormonal treatment, highlighting the importance of obtaining native samples for future studies regarding its role in the development and presentation of endometriosis. However, hs-CRP and other studied biomarkers seemed to be of no value for the assessment and diagnosis of endometriosis.

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“…Due to this hypothesis, there is a growing interest to identify and validate biological markers that help in diagnosing functional GI disorders, such as IBS (Mujagic et al, 2016). One of those markers which proved to be significantly altered in IBS patients compared to individuals in the healthy control group is fecal calprotectin, pointing to a low-grade pro-inflammatory state in an IBS patient subgroup (Mujagic et al, 2016;Pletikosic, Plavsic, Hauser, & Tkalcic, 2015). Furthermore, our preliminary results (Pletikosic et al, 2015) indicate that fecal calprotectin levels significantly correlate with the physical component of healthrelated quality of life (HRQoL).…”

Section: Introductionmentioning

confidence: 99%

Brain-Gut Miscommunication

Tončić

Tkalčić

Hauser

2018

Psihol. teme (Online)

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Irritable bowel syndrome (IBS) is a complex disorder that results from interactions of numerous factors. The biopsychosocial model describes a number of predisposing, precipitating, and perpetuating factors, which contribute to the onset and maintenance of symptoms and consequently to quality of life (QoL) impairment. The aim of this study was to examine the impact of several psychological and biological factors on the physical and mental components of QoL in IBS patients. A total of 46 IBS patients completed a set of questionnaires (Big Five Inventory, State-Trait Anxiety Inventory, Beck Depression Inventory-II, Medical Outcome Study Short-Form 36) and kept a diary of their mood, daily stress, and symptoms over a period of two weeks. Patients' heart rate variability, serum cortisol, and fecal calprotectin levels were also measured. The results of regression analyses showed that depression (β = -.30) and negative mood (β = -.28) predicted physical QoL, while depression (β = -.45) and positive mood (β = .33) significantly predicted mental QoL. The model, which included calprotectin, cortisol, anxiety, depression, and positive and negative mood, explained a total of 47% of variance of physical and 57% of variance of mental QoL. Our results confirm the role of negative affect in IBS QoL impairment. They also indicate that biological factors seem important for physical QoL in IBS patients. The role of positive mood as a protective factor for mental QoL might be significant for psychological interventions with IBS patients.

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Fecal Calprotectin and serum chromogranin A as potential biomarkers of irritable bowel syndrome symptom severity

Cited by 5 publications

References 42 publications

The microbiome in adolescents with irritable bowel syndrome and changes with percutaneous electrical nerve field stimulation

The microbiome in adolescents with irritable bowel syndrome and changes with percutaneous electrical nerve field stimulation

Plasma AXIN1 expression exhibit negative correlations with inflammatory biomarkers and is associated with gastrointestinal symptoms in endometriosis

Brain-Gut Miscommunication

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