Fecal Bacteria in Hokkaido Native Horses as Characterized by Microscopic Enumeration and Competitive Polymerase Chain Reaction Assays.

Koike, Satoshi; Shingu, Yuko; Inaba, Hiroyuki; Kawai, Masahito; Kobayashi, Yasuo; Hata, Hiroshi; Tanaka, Keiichi; Okubo, Masahiko

doi:10.1294/jes.11.45

Cited by 21 publications

(32 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although there is no detailed comparative study, we assumed that fecal microbiota could reflect hindgut microbiota to some extent, since no significant difference in microscopic enumeration was observed between the rectum and colon of horses (Koike et al 2000). This was also reported by Kern et al (1974) who investigated microbes of the terminal colon (close to the rectum) and cecum of the pony.…”

Section: Introductionmentioning

confidence: 63%

“…However, there was a clear shift in the proportion of Gram-negative rods, showing significant increases in the winter seasons (Table 1). Koike et al (2000) found that Hokkaido native horses have a bacterial adaptation to low-quality roughage diets on winter woodland pasture in their increased levels of Fibrobacter succinogenes, one of the most potent fibrolytic species present in the equine hindgut. They also observed an increase in Gram-negative rods in winter (Koike et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Koike et al (2000) found that Hokkaido native horses have a bacterial adaptation to low-quality roughage diets on winter woodland pasture in their increased levels of Fibrobacter succinogenes, one of the most potent fibrolytic species present in the equine hindgut. They also observed an increase in Gram-negative rods in winter (Koike et al 2000). Since F. succinogenes is categorized with the Gram-negative rods, the increased proportion of this group in winters of the present study (Table 1) may be partly explained by the increase in F. succinogenes in winter of up to 1.0-4.4% of total bacteria (Koike et al 2000).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Hindgut microbes, fermentation and their seasonal variations in Hokkaido native horses compared to light horses

Kobayashi

Koike

Miyaji

et al. 2005

Ecological Research

Self Cite

View full text Add to dashboard Cite

Fecal bacteria and protozoa of Hokkaido native horses and light horses were enumerated to compare seasonal variation in hindgut microbes and fermentation between the two breeds. Fecal samples were collected in winter and summer from eight horses (four for each breed) that had been reared together under the same conditions after birth (on woodland pasture in winter and on grassland pasture for the rest of the year). Total fecal bacteria counts for both breeds showed temporal variation, with the highest levels occurring in summer (P<0.05). For both breeds, Gram-negative rods were the major constituents (58–69%) and showed higher counts in winter (P<0.05) than in summer. Total protozoa counts in both breeds were lower in winter than in summer (P<0.05). The proportion of large cellulolytic protozoa such as Cochliatoxum periachtum was increased (P<0.05) in winter, and this tended to be more pronounced in native horses. Although total volatile fatty acids (VFA) in feces were lower in winter (P<0.05), the reduction was smaller in native horses (P<0.05). Fecal VFA pattern showed a shift toward more acetate and less propionate production in winter regardless of the horse breed. Evaluation of digestive tract organs in 12 animals showed that the relative weight of the colon in body weight or total digestive tract weight is larger in native horses than in light horses (P<0.05). The present results suggest that hindgut microbial adaptation to winter diets occurs to a greater extent in native horses, as partly characterized by advantages in anatomy

show abstract

Section: Introductionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hindgut microbes, fermentation and their seasonal variations in Hokkaido native horses compared to light horses

Kobayashi

Koike

Miyaji

et al. 2005

Ecological Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…The microbial ecology of foregut fermenters is researched extensively (Sylvester et al 2004;Denman & McSweeney 2006;Mosoni et al 2007;Wanapat & Cherdthong 2009). However, the literature regarding microbial ecology of hindgut fermenters is limited to horses (Koike et al 2000;Daly et al 2001;Yamano et al 2008). Results of present study serve as a baseline data for future research in this direction.…”

Section: Gut Microbial Profilementioning

confidence: 99%

Influence of work on nutrient utilisation in semicaptive Asian elephants (Elephas maximus)

Katole

Das

Agarwal

et al. 2014

Journal of Applied Animal Research

View full text Add to dashboard Cite

Two feeding trials were conducted to evaluate the feed consumption, nutrient utilisation, blood biochemical and faecal microbial profile of working and nonworking semicaptive Asian elephant. During each trial, six captive elephants were placed in two groups of three each. Elephants in one group performed the scheduled work at the park, i.e., 4-h safari with tourists, while the other group performed no work. During nighttime, all the elephants were kept in respective individual enclosure so that feed consumption and faeces voided could be measured accurately. During day time, all the elephants were allowed to forage in nearby forest. Intake (kg/d) of sugarcane (Saccharum officinarum) and sugarcane leaves was more (P < 0.01) in nonworking elephants as compared to working elephants. Working elephants consumed more (P < 0.01) forages during foraging than nonworking elephants. Average daily dry matter intake (DMI) and DMI (% body weight, BW) were comparable between the groups. Apparent digestibility (%) of DM, OM, crude protein (CP), neutral detergent fibre (NDF), acid detergent fibre (ADF), hemicellulose, cellulose and gross energy (GE) were higher (P < 0.01) in working than nonworking elephants. Activity of ALT (IU/l) was higher in working as compared to nonworking elephants. The relative population of Fibrobacter succinogenes and Ruminococcus flavefaciens and total fungi were numerically increased in working elephants as compared to nonworking elephants. It was concluded that 4 h of work has no adverse impact on food consumption and blood metabolite profile of semicaptive Asian elephants; rather it improved the digestibility of nutrients. Work showed positive effect in restricting the calorie supply closer to requirement.

show abstract

“…As amilolíticas, principalmente Lacobacillus e Streptococcus digerem principalmente carboidratos que escapam à digestão enzimática no intestino delgado. Koike et al (2000) encontraram no ceco de cavalos bactérias celulolíticas principalmente de cepas específicas de A Odontoplastia é baseada inicialmente na retomada do equilíbrio da oclusão, através do desgaste da coroa dentária dos molares e incisivos, o tratamento consiste em estabelecer uma mordida coerente sem sobrecarregar a articulação temporomandibular, todos os procedimentos nesta área visam aumentar o conforto do cavalo. O primeiro passo para este tipo de intervenção é a realização de um exame físico do animal e anamnese detalhada para garantir que não existem outras afecções envolvidas.…”

Section: Digestão Dos Carboidratosunclassified

<b>Efeito do tratamento odontológico sobre parâmetros digestivos e metabólicos de equinos</b>

Filho¹,

de²

View full text Add to dashboard Cite

DEDICATÓRIADedico esse trabalho exclusivamente aos meus pais Luiz A. J. de Moraes e Telma S. de Almeida J. de Moraes e a minha irmã Lais J. de Moraes, pois sempre estiveram ao meu lado me incentivando e me apoiando, e se existem vitórias na minha vida, são todas dedicadas ao amor que essa família sempre me deu! Changes in feed for horses led to change also the natural tooth wear, making it cluttered and even inefficient, contributing broadly to digestive disorders. The aim of this study was to investigate the implications of dental treatment on the digestive and metabolic parameters of horses. Eight horses, Arabian race, castrated male, mean age 80 ± 7 months and average weight of 460 ± 28 kg never undergoing dental treatment were used. The animals were housed in individual stals, fed a diet consisting of 2% of body weight (BW) in MS/day, divided into 0.75% of pelletized concentrate formulated for equine maintenance and 1.25% grass hay ( Cynodon sp. cv. Tifton 85). RESUMOThe treatments were divided into control group (D0; animals without any dental treatment), D20 group (animals evaluated twenty days after dental treatment) and D40 group (animals evaluated forty days after the dental treatment). The animals went through a period of adjustment to the 15-day diet and five days of total collection. The floating teeth consisted of sedation and, as a result, reduction of excessive enamel points and occlusion adjustment, seeking to improve the range of masticatory movements. The variables evaluated were: apparent digestibility coefficient (CD) of dry matter (DM), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP) and ether-extract (EE); area under the curve (AUC) of glucose and insulin, ingestive behavior, cholesterol, triglycerides, and lipoproteins concentration, fecal pH, short chain fatty acids (SCFA) and lactic acid in the stool. The experimental design was completely randomized with repeated measures time, and data were submitted to analysis of variance considering a 5% significance. There were differences in apparent digestibility (P<0.05) with reduction in the ADF in D20 and D40 groups. For SCFA profile, floating teeth increased propionic acid D40 group compared to the control and reduction of lactic acid in the feces of the D40 group compared to the control. The animals of group D40 had weight reduction (P<0.05).The odontoplastia did not interfere in the remaining parameters. It can be concluded that after floating teeth there were changes in voluntary intake of roughage, which promotes change in concentrate/roughage diet of horses, interfering in the fermentation environment and postprandial metabolic.

show abstract

Fecal Bacteria in Hokkaido Native Horses as Characterized by Microscopic Enumeration and Competitive Polymerase Chain Reaction Assays.

Cited by 21 publications

References 12 publications

Hindgut microbes, fermentation and their seasonal variations in Hokkaido native horses compared to light horses

Hindgut microbes, fermentation and their seasonal variations in Hokkaido native horses compared to light horses

Influence of work on nutrient utilisation in semicaptive Asian elephants (Elephas maximus)

<b>Efeito do tratamento odontológico sobre parâmetros digestivos e metabólicos de equinos</b>

Contact Info

Product

Resources

About