Febrile infection changes the expression of IgG Fc receptors and complement receptors in human neutrophils <i>in vivo</i>

Leino, Lasse; Sorvajärvi, Katja; Katajisto, Johanna; Laine, Merja; Lilius, Esa‐Matti; Pelliniemi, Tarja‐Terttu; Rajamäki, Allan; Silvoniemi, P.; Nikoskelainen, J

doi:10.1046/j.1365-2249.1997.d01-899.x

Cited by 59 publications

(43 citation statements)

References 36 publications

(33 reference statements)

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“…Earlier studies indicated that adult PB PMN expressed very low levels of Fc␥RI (35), a result consistent with our data. The up-regulation of Fc␥RI expression on PMN was observed for patients with febrile bacterial infections (31) and was shown to be induced by IFN-␥ (21,50). Human inflamed gingival tissue contains ␥␦ T cells expressing IFN-␥ mRNA (34,61).…”

Section: Discussionmentioning

confidence: 99%

Effective In Vitro Clearance ofPorphyromonas gingivalisby Fcα Receptor I (CD89) on Gingival Crevicular Neutrophils

et al. 2001

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Porphyromonas gingivalis has been implicated as a causative pathogen in periodontitis. Immunotherapeutic approaches have recently been suggested to aid in the clearance of P. gingivalis from disease sites. Because antibody-Fc receptor (FcR) interactions play a role in the effector functions of polymorphonuclear neutrophils (PMN), we evaluated which FcR on PMN from gingival crevicular fluid (GCF) serves as an optimal target molecule for FcR-directed immunotherapy. GCF PMN and peripheral blood (PB) PMN from adult periodontitis patients were analyzed for their immunoglobulin G (IgG) and IgA FcR (Fc␥R and Fc␣R, respectively) expression and function by studying IgG-and IgA-mediated elimination of P. gingivalis. GCF PMN exhibited higher Fc␣RI and Fc␥RI levels and lower Fc␥RIIa and Fc␥RIIIb levels than PB PMN. Functional studies revealed that GCF PMN exhibited less of a capacity to phagocytose and kill IgG1-opsonized P. gingivalis than PB PMN. IgA1-mediated phagocytosis and killing capacity was, however, comparable between GCF PMN and PB PMN. In summary, these in vitro results document that Fc␣RI represents a candidate target for FcRdirected immunotherapy for the clearance of P. gingivalis.

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Section: Discussionmentioning

confidence: 99%

Effective In Vitro Clearance ofPorphyromonas gingivalisby Fcα Receptor I (CD89) on Gingival Crevicular Neutrophils

et al. 2001

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“…During bacterial infection, the expression of CD64 on activated neutrophils was markedly increased (18,33,34). More important, it has also been shown that neutrophils of preterm infants expressed CD64 antigen to an extent similar to those of older children and adults (18).…”

Section: Diagnosis Of Neonatal Infection By Cd64mentioning

confidence: 98%

Neutrophil CD64 Expression: A Sensitive Diagnostic Marker for Late-Onset Nosocomial Infection in Very Low Birthweight Infants

et al. 2002

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This study aims to evaluate the diagnostic utilities of four leukocyte surface antigens-two lymphocyte antigens (CD25 and CD45RO) and two neutrophil antigens (CD11b and CD64)-for identification of late-onset nosocomial bacterial infection in preterm, very low birthweight infants, and to define the optimal cutoff value for each marker so that it may act as a reference with which future studies can be compared. Very low birthweight infants in whom infection was suspected when they were Ͼ72 h of age were eligible for the study. A full sepsis screen was performed in each episode. IL-6, C-reactive protein, and leukocyte surface antigens (CD25, CD45RO, CD11b, and CD64) were measured at 0 (at the time of sepsis evaluation), 24, and 48 h by standard biochemical methods and quantitative flow cytometric analysis. The diagnostic utilities including sensitivity, specificity, and positive and negative predictive values of each marker and combination of markers for predicting late-onset neonatal infection were determined. One hundred twenty-seven episodes of suspected clinical sepsis were investigated in 80 infants. Thirty-seven episodes were proven infection. The calculated optimal cutoff values for CD25, CD45RO, CD11b, and CD64 were 3,100, 2,900, 10,450, and 4,000 phycoerythrinmolecules bound per cell, respectively. An interim analysis of data after 68 episodes suggested that CD25 and CD45RO were poor predictors of neonatal infection with sensitivity or specificity Ͻ75% during a single measurement. Thus, these two markers were excluded from further investigation. In the final analysis, CD64 has the highest sensitivity (95-97%) and negative predictive value (97-99%) at 0 and 24 h after the onset. The addition of IL-6 or C-reactive protein (0 h) to CD64 (24 h) further enhanced the sensitivity and negative predictive value to 100%, and has the specificity and positive predictive value exceeding 88% and 80%, respectively. Neutrophil CD64 expression is a very sensitive marker for diagnosing late-onset nosocomial infection in very low birthweight infants. If further validated, the use of CD64 as an infection marker should allow early discontinuation of antibiotic treatment at 24 h without waiting for the definitive microbiologic culture results. The quantitative flow cytometric analysis applied in this study could be developed into a routine clinical test with high comparability and reproducibility across different laboratories. Vol. 51, No. 3, 2002 Copyright © 2002 Printed in U.S.A. ABSTRACT296

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“…The expression of leukocyte cell-surface receptors was measured by flow cytometry as described earlier [22]. Briefly, aliquots of leukocyte suspension (containing 300,000 leukocytes) were labeled with fluorescein isothiocyanate (FITC)-conjugated anti-FcRIII (Caltag, San Francisco, CA), anti-CR1 (CLB, Amsterdam, The Netherlands), anti-L-selectin (Becton-Dickinson, San Jose, CA), and phycoerythrin (PE)-conjugated anti-FcRII (Caltag) and anti-CR3 (Becton-Dickinson) mAbs for 30 min at 4°C.…”

Section: Measurement Of Receptor Expressionmentioning

confidence: 99%

Systemic suppression of human peripheral blood phagocytic leukocytes after whole-body UVB irradiation

Leino

Saarinen

et al. 1999

Journal of Leukocyte Biology

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We examined systemic effects of wholebody UVB irradiation on human peripheral blood phagocytes. We found that 24 h after a single erythemal dose of UVB radiation two phagocyte functions, adhesion and phagocytosis, were reduced by 50%. This functional suppression was accompanied by a significant decrease in the expression of complement receptors (CR1 and CR3) and IgG Fc receptors (FcRII and FcRIII). The greatest reduction (47%) was observed in CR3, which is important for both adhesion and phagocytosis. A kinetic analysis showed that both CR1 and CR3 levels started to decrease 15 min after the UVB exposure, reaching the lowest levels at 4.5-and 24-h time points, respectively. The down-modulation of CRs after whole-body UVB exposure was not due to a defective receptor synthesis or translocation from internal stores to plasma membrane because the maximal CR levels in stimulated cells were not affected by UVB. No change in the serum soluble ICAM-1 was detected after UVB, which rules out CD11b epitope masking by sICAM-1. UVB did not release low-receptor-density myeloid progenitor cells from storage pools into circulation. Interleukin 10, a mediator of UVB-induced immunosuppression, was unable to modulate CR expression in vitro. When seven suberythemal whole-body UVB exposures were given repeatedly within 2 weeks, a significant decrease in CR expression was seen, which was greatest after three irradiations. Our data suggest that an exposure to UVB has systemic effects in humans which, possibly due to the down-modulation of preexisting cell-surface receptors, suppress some important functions of circulating phagocytic cells. J. Leukoc. Biol. 65: 573-582; 1999.

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Febrile infection changes the expression of IgG Fc receptors and complement receptors in human neutrophils in vivo

Cited by 59 publications

References 36 publications

Effective In Vitro Clearance ofPorphyromonas gingivalisby Fcα Receptor I (CD89) on Gingival Crevicular Neutrophils

Effective In Vitro Clearance ofPorphyromonas gingivalisby Fcα Receptor I (CD89) on Gingival Crevicular Neutrophils

Neutrophil CD64 Expression: A Sensitive Diagnostic Marker for Late-Onset Nosocomial Infection in Very Low Birthweight Infants

Systemic suppression of human peripheral blood phagocytic leukocytes after whole-body UVB irradiation

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