Features of the Processes of Lipoperoxidation and Antioxidant Protection in the Pathogenesis of Infertility in Men of Different Ethnic Groups, Carriers of Nonfunctional Polymorphisms of the GSTT1 and GSTM1 Genes

Abstract

A comparative analysis was carried out of the parameters of lipoperoxidation and antioxidant protection in fertile and infertile men of two ethnic groups (Caucasians and Mongoloids), who were carriers of different genotypes of xenobiotic biotransformation genes (GSTT1 and GSTM1). In serum and ejaculate, we determined the content of TBA-active products, α-tocopherol, reduced glutathione (GSH) and oxidized glutathione (GSSG); the activity of glutathione-S-transferase (GST), glutathione peroxidase (GPO) and glutathione reductase (GR); and total antioxidant activity (AOA). It was found that in Caucasian fertile men, carriers of genotypes GSTT1(*0/*0)/GSTM1(*0/*0), there was a decrease in GST activity in serum and ejaculate with an increase in AOA in these substrates in comparison with similar indicators in carriers of genotypes GSTT1(1+/1+)/GSTM1(1+/1+). In Mongoloid fertile men, carriers of genotypes GSTT1(*0/*0)/GSTM1(*0/*0), in comparison with similar indices in carriers of genotypes GSTT1(1+/1+)/GSTM1(1+/1+), we found an increase in serum content of α-tocopherol and activity of GPO, a decrease in GST activity, and a decrease in the level of α-tocopherol in the ejaculate. In Caucasians with infertility, carriers of genotypes GSTT1(*0/*0)/GSTM1(0*/*0), we found decreased activity of GST in the blood and ejaculate. In this group, we also registered a decrease in the GSH level, with an increase in the level TBA-active products and GPO activity. In infertile Mongoloids, carriers of genotypes GSTT1(*0/*0)/GSTM1(*0/*0), we found an increase in the level TBA-active products and GPO activity and a decrease in serum α-tocopherol level.

Association of Lipid Peroxidation/Antioxidant System Activity with Glutathione S-Transferase P1 Polymorphisms in Infertile Men

“…(16,21,23) Xenobiotic detoxification system enzymes are involved in metabolic reactions aimed at reducing the activity of foreign substances; the deletion polymorphism of the GST gene family can contribute to the formation of reproductive disorders in men. (29,30) An analysis of the frequency with which the Ile105Val polymorphism of the GSTP1 gene occurred in men with infertility and in fertile men found statistically significant differences (χ 2 =7.487; P=0.024) ( Table 1) . Men in the control group with proven fertility have a homozygous Ile105Ile genotype in 54% of cases, while in men with infertility this genotype was observed in 67% of cases.…”