Features of the Interaction between Cyclodextrins and Colloidal Liposomes

Puskás, István; Barcza, Lajos; Szente, Lajos; Csempesz, Ferenc

doi:10.1007/s10847-005-4805-6

Cited by 16 publications

(23 citation statements)

References 6 publications

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“…Apparently, β-CD acts as a destabilizing agent for POPC liposomes, probably by including POPC (Puskás and Csempesz, 2007;Irie et al, 1992) molecules into its cavity and, consequently, causing a reorganization of the molecular packing in the liposomal membrane. Clear evidence of the tendency of POPC molecules to form complexes with β-CD has been obtained from ESI-MS experiments (see Figure 4), in agreement with recent findings, through binding isotherms, on the interaction between various CDs and DPPC (1,2-dipalmitoylsn-glycero-3-phosphocholine) SUV liposomes (Puskás et al, 2006).…”

Section: Stability Of the Liposomessupporting

confidence: 90%

“…Probably, the first factor is the most relevant one, as, even though the published data for the complex stability constants with β-CD are 17,000 M −1 (Frijlink et al, 1991) and about 300 M −1 (Puskás et al, 2006) for cholesterol/β-CD and phospholipid/β-CD 1:1 complex, respectively, the 10 times higher concentration of POPC over cholesterol prevents complexation of the latter.…”

Section: Stability Of the Liposomesmentioning

confidence: 99%

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Fine-tuning of POPC liposomal leakage by the use of β-cyclodextrin and several hydrophobic guests

Gasbarri

Guernelli

Boncompagni

et al. 2009

Journal of Liposome Research

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The effect of entrapped beta-cyclodextrin (beta-CD) on the stability of multilamellar vesicles (MLVs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), prepared by the dehydration-rehydration method, was studied by monitoring the release of 5(6)-carboxyfluorescein encapsulated into the liposomes. Different hydrophobic guests, such as Fullerene C(60), have been incorporated into the POPC bilayer in order to modify the membrane composition. The kinetic results as well as ESI-MS measurements evidenced that the destabilizing activity of beta-CD is due to the formation of beta-CD inclusion complexes and the consequent removal of selected bilayer constituents from the liposomal membrane. Hence, when beta-CD was added to the liposomes in the form of a strong, water-soluble 2:1 beta-CD/C(60) inclusion complex, such a destabilizing effect was not observed. However, the same beta-CD/C(60) inclusion complex does not form as a result of C(60) extraction from the bilayer. This may be attributed either to the overwhelming concentration of POPC with respect to C(60) and/or to the fact that C(60) is largely aggregated in the bilayer. Turbidimetric and fluorimetric determinations of lamellarity and entrapped volume of the studied MLVs provided further evidence of the alteration of the liposomal bilayer as a consequence of the addition of beta-CD and/or the presence of the studied guests.

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Section: Stability Of the Liposomessupporting

confidence: 90%

Section: Stability Of the Liposomesmentioning

confidence: 99%

Fine-tuning of POPC liposomal leakage by the use of β-cyclodextrin and several hydrophobic guests

Gasbarri

Guernelli

Boncompagni

et al. 2009

Journal of Liposome Research

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“…Ohvo and Slotte [23] demonstrated that the desorption rates of various sterols from the surface film to b-CD solution depend on the relative polarity of sterols and increase considerably with the surface pressure rise. Moreover, sterol desorption from cholesterol/phospholipid mixed systems was found to be notably retarded, even though the removal of phospholipid molecules was not observed [23][24][25][26][27][28][29][30]. On the contrary, phospholipids' desorption from monolayers has recently been described by other authors [31].…”

Section: Introductionmentioning

confidence: 82%

“…Langmuir monolayers [21][22][23][24][25][26] and lipid vesicles [27][28][29]. The depletion of membrane constituents, namely cholesterol, phospholipids and even proteins from cells was described in several papers.…”

Section: Introductionmentioning

confidence: 99%

X-ray grazing incidence diffraction and Langmuir monolayer studies of the interaction of β-cyclodextrin with model lipid membranes

Flasiński

Broniatowski

Majewski

et al. 2010

Journal of Colloid and Interface Science

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“…The structural changes were also assessed using differential scanning calorimetry [48], freeze-fracture electron microscopy [47], binding isotherms [49] or photon-correlation spectroscopy [19,23,49,50]. Despite all these studies, rare are those involving specifically the interaction between b-CD and phospholipids [19,49,51]. And, to the best of our knowledge, only information on the global liposome size and dispersity could be inferred from the previously mentioned techniques which do not give access to the internal structure of the bilayer.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the interaction between a β-cyclodextrin and DMPC liposomes: a small angle neutron scattering study

Joset

Grammenos

Hoebeke

et al. 2015

J Incl Phenom Macrocycl Chem

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The small angle neutron scattering technique has been applied to investigate the interaction between a cyclodextrin (CD) and liposomes. From the modelling of the experimental neutron scattering cross sections, the detailed structure of dimyristoylphosphatidylcholine (DMPC) liposomes is assessed upon addition of increasing amounts of randomly methylated b-CD (RAMEB). This study has been performed at two temperatures bracketing the phase transition of the DMPC bilayers. The fraction of DMPC molecules incorporated into the vesicles is inferred. The dose-dependent phospholipidic extraction by RAMEB is quantified as well as the concomitant evolution of the liposome radius and of the thickness of the hydrophobic and hydrophilic parts of the membrane. The possible formation of CD-DMPC inclusion complexes is also assessed. The data suggest the dose-dependent coverage by RAMEB of the outer liposome interface. Our analysis highlights the important role of temperature on the mechanism of action of RAMEB. These results are discussed in the framework of the area-difference-elasticity model.

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Features of the Interaction between Cyclodextrins and Colloidal Liposomes

Cited by 16 publications

References 6 publications

Fine-tuning of POPC liposomal leakage by the use of β-cyclodextrin and several hydrophobic guests

Fine-tuning of POPC liposomal leakage by the use of β-cyclodextrin and several hydrophobic guests

X-ray grazing incidence diffraction and Langmuir monolayer studies of the interaction of β-cyclodextrin with model lipid membranes

Investigation of the interaction between a β-cyclodextrin and DMPC liposomes: a small angle neutron scattering study

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