Features of development of satellite networks of broadband access (BBA) in the Ka-range

Afonin, Alexander A.; Shevtsov, V. A.

doi:10.1109/icton.2011.5970857

Cited by 5 publications

(6 citation statements)

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“…177 The self-assembling hexameric nanorings represent a widely studied model of RNA NANP. 137,138 Fully assembled nanorings consist of six dumbbell-like units connected by RNA kissing loop-loop interactions. 137 Formation of the functional light-up split aptamer confirmed the assembly of nanorings, whereas the maximum fluorescence occurred only when a fully assembled ring was formed.…”

Section: Light-up Aptamers As Trackers Of Nanp Assemblymentioning

confidence: 99%

“…Versatility of the technique was confirmed in mammalian cells and in vivo with the triggering of various functionalities and silencing of several genes (HIV-1 genes, eGFP, or glutathione S-transferaseP1), fluorescence resonance energy transfer (FRET), and aptamer binding the malachite green ( Figure 6C). 179 Physical interactions of sense and antisense parts leads to the interaction of respective complementary counterparts by toehold interactions and restores functionality. 180 One of the ways to monitor this process is the splitting of the Broccoli aptamer sequence into two separate nonfunctional strands (called Broc and Coli).…”

Section: Light-up Aptamers As Trackers For Intracellular Activation Omentioning

confidence: 99%

“…131 Computer-assisted rational design tremendously expands the possibilities for generating versatile NANPs, thus creating an alternative approach to pRNA-based designs. 61,[132][133][134] The compact multifunctional RNA NANPs designed in silico, 135 extensively tested in vitro, [136][137][138] and then demonstrated in animal models 60 have the capacity to simultaneously carry on a combination of several aptamers ( Figure 5B) and/or RNAi inducers designed to simultaneously silence up to six different genes required, for example, in HIV-1 replication. 60 Recently, the simple modification of NANP structures allowed for doubling the number of functionalities per the same construct.…”

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Aptamers as Modular Components of Therapeutic Nucleic Acid Nanotechnology

et al. 2019

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Nucleic acids play a central role in all domains of life, either as genetic blueprints or as regulators of various biochemical pathways. The chemical makeup of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA), generally represented by a sequence of four monomers, also provides precise instructions for folding and higher-order assembly of these biopolymers that, in turn, dictate biological functions. The sequence-based specific 3D structures of nucleic acids led to the development of the directed evolution of oligonucleotides, SELEX (systematic evolution of ligands by exponential enrichment), against a chosen target molecule. Among the variety of functions, selected oligonucleotides named aptamers also allow targeting of cell-specific receptors with antibody-like precision and can deliver functional RNAs without a transfection agent. The advancements in the field of customizable nucleic acid nanoparticles (NANPs) opened avenues for the design of nanoassemblies utilizing aptamers for triggering or blocking cell signaling pathways or using aptamer-receptor combinations to activate therapeutic functionalities. A recent selection of fluorescent aptamers enables real-time tracking of NANP formation and interactions. The aptamers are anticipated to contribute to the future development of technologies, enabling an efficient assembly of functional NANPs in mammalian cells or in vivo. These research topics are *

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Section: Light-up Aptamers As Trackers Of Nanp Assemblymentioning

confidence: 99%

Section: Light-up Aptamers As Trackers For Intracellular Activation Omentioning

confidence: 99%

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Aptamers as Modular Components of Therapeutic Nucleic Acid Nanotechnology

et al. 2019

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“…RNA 3WJ and other nanoparticles have the ability to self-assemble co-transcriptionally under isothermal conditions. 43,49,72,[87][88][89] To assemble the pRNA-3WJ co-transcriptionally, 3WJ-a, 3WJ-b, and 3WJ-c sequences were incorporated in separate circular dsDNA constructs. All constructs demonstrated cleavage and release of the product RNA ( Figure 4A, red box).…”

Section: One-pot Co-transcriptional Assembly Of 3wj Nanoparticlesmentioning

confidence: 99%

“…71 Additionally, in vitro transcription is not limited by length, and one-stranded or multistranded nanoparticle assembly can occur co-transcriptionally, reducing the total number of steps required for RNA nanoparticle preparation. 43,49,72 However, previous methods of circular DNA preparation for RCT are not amenable for ssDNA templates displaying stable secondary structure. Bacterial RNA polymerases are sensitive to secondary structure, falling off template DNA when encountering DNA hairpins and loops.…”

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One-Pot Production of RNA Nanoparticles via Automated Processing and Self-Assembly

2019

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From the original sequencing of the human genome, it was found that about 98.5% of the genome did not code for proteins. Subsequent studies have now revealed that a much larger portion of the genome is related to short or long noncoding RNAs that regulate cellular activities. In addition to the milestones of chemical and protein drugs, it has been proposed that RNA drugs or drugs targeting RNA will become the third milestone in drug development (Shu, Y.; et al. Adv. Drug Deliv. Rev. 2014, 66, 74.). Currently, the yield and cost for RNA nanoparticle or RNA drug production requires improvement in order to advance the RNA field in both research and clinical translation by reducing the multiple tedious manufacturing steps. For example, with 98.5% incorporation efficiency of chemical synthesis of a 100 nucleotide RNA strand, RNA oligos will result with 78% contamination of aborted byproducts. Thus, RNA nanotechnology is one of the remedies, because large RNA can be assembled from small RNA fragments via bottom-up selfassembly. Here we report the one-pot production of RNA nanoparticles via automated processing and self-assembly. The continuous production of RNA by rolling circle transcription (RCT) using a circular dsDNA template is coupled with self-cleaving ribozymes encoded in the concatemeric RNA transcripts. Production was monitored in real-time. Automatic production of RNA fragments enabled their assembly either in situ or via one-pot co-transcription to obtain RNA nanoparticles of desired motifs and functionalities from bottom-up assembly of multiple RNA fragments. In combination with the RNA nanoparticle construction process, a purification method using a largescale electrophoresis column was also developed.

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Optical and Microwave Technologies for Telecommunication Networks

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Features of development of satellite networks of broadband access (BBA) in the Ka-range

Cited by 5 publications

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Aptamers as Modular Components of Therapeutic Nucleic Acid Nanotechnology

Aptamers as Modular Components of Therapeutic Nucleic Acid Nanotechnology

One-Pot Production of RNA Nanoparticles via Automated Processing and Self-Assembly

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