Features of adsorption of globular proteins by nanoporous silica

El'tekova, N. A.; El’tekov, A. Yu.

doi:10.1134/s2070205110010089

Cited by 5 publications

(6 citation statements)

References 14 publications

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“…When the pore average size is more than two and a half times larger than that of the molecule, adsorption increases less dramatically with an increase in the average pore diameter. Such a dependence of the BSA adsorption on the silica adsorbents pore size (porous glasses, silica gels and silochromes) was obtained in [5]. According to the authors of [5], the maximum value of BSA adsorption is 1.1 mg/m 2 , which is close to the value of hemoglobin adsorption on silochrome S 120 and somewhat less than those of macroporous silica gel and silochrome obtained in the present work.…”

Section: Resultssupporting

confidence: 64%

“…Such a dependence of the BSA adsorption on the silica adsorbents pore size (porous glasses, silica gels and silochromes) was obtained in [5]. According to the authors of [5], the maximum value of BSA adsorption is 1.1 mg/m 2 , which is close to the value of hemoglobin adsorption on silochrome S 120 and somewhat less than those of macroporous silica gel and silochrome obtained in the present work. The molecular weights and molecule sizes for BSA and hemoglobin are similar.…”

Section: Resultssupporting

confidence: 64%

“…The molecular weights and molecule sizes for BSA and hemoglobin are similar. The slight difference in the maximum adsorption values of these proteins could be due to the different experimental conditions (6°С in the present work and 20°С in [5]). Alumina is an exception from the general regularity shown in Fig.…”

Section: Resultsmentioning

confidence: 57%

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Adsorption properties of hemoglobin

Atyaksheva

Dobryakova

Ivanova

et al. 2012

Russ. J. Phys. Chem.

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Section: Resultssupporting

confidence: 64%

Section: Resultssupporting

confidence: 64%

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Adsorption properties of hemoglobin

Atyaksheva

Dobryakova

Ivanova

et al. 2012

Russ. J. Phys. Chem.

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“…Interaction of solvent molecules with polymer and adsorbent in this adsorption system seems to be weakened. Lability of more compacted macromolecular coil increases encouraging greater adsorption of PS under these conditions [3,[13][14][15].…”

Section: Resultsmentioning

confidence: 99%

“…Under static conditions, adsorption from diluted solutions was studied with the help of an interferometer by changes in the polymer concentration [13][14][15]. Chromatographic experiments were run using liquid chromatograph Waters-200 (column 1.2 m, particle size 85+5 µm) and liquid chromatograph LC-1309 (NTO, S-Peterburg, Russia) (column 300x0.5 mm, particles size 5+2 µm).…”

Section: Methodsmentioning

confidence: 99%

Effect of temperature on adsorption and chromatography of polystyrene macromolecules

El’tekov¹

2018

sorpchrom

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Study of the effect of temperature on the parameters of adsorption and liquid chromatography of molecules and macromolecules constantly attracts the attention of specialists. The potential benefits of elevated temperature column, particularly enhanced kinetic and transport properties, which are based on the reduction of mobile phase viscosity and increase the diffusion of analyte at high temperature, began to be actively used for the rapid analysis of molecules and macromolecules by chromatographic methods in recent years. It is now recognized that temperature is an important tool to optimize chromatographic parameters, such as retention, selectivity and efficiency, particularly for macromoleculesThe effect of temperature on adsorption and liquid chromatography of polystyrene macromolecules on silicas has been investigated by static and .chromatographic methods. The sign and value of the temperature adsorption coefficient depend on many factors, namely, the chemical nature of the solvent, the chemical structure of macromolecules and adsorbent surface chemistry. Thus temperature elevation in the chromatographic system alters the macromolecular coil dimensions and total energy of elution of solvent molecules from the adsorption space of the porous adsorbents. Keeping these factors in view we can find the sign of the temperature adsorption coefficient. Diffusion coefficients increase in proportion to temperature, thus efficiency is usually enhanced, too, as peaks become more narrow. Prospects for optimization of the chromatographic process and, above all, the efficiency of separation of components of mixtures, include the effect of temperature on the adsorption of the components and their retention in the chromatographic column.

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