Features and mechanisms of propofol-induced protein kinase C (PKC) translocation and activation in living cells
Soma Noguchi,
Taketoshi Kajimoto,
Takuya Kumamoto
et al.
Abstract:Background and purpose: In this study, we aimed to elucidate the action mechanisms of propofol, particularly those underlying propofol-induced protein kinase C (PKC) translocation.Experimental approach: Various PKCs fused with green fluorescent protein (PKC-GFP) or other GFP-fused proteins were expressed in HeLa cells, and their propofol-induced dynamics were observed using confocal laser scanning microscopy. Propofol-induced PKC activation in cells was estimated using the C kinase activity receptor (CKAR), an… Show more
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