Featured Gut Microbiomes Associated With the Progression of Chronic Hepatitis B Disease

Chen, Zhangran; Xie, Yurou; Zhou, Fei; Zhang, Bangzhou; Wu, Jingtong; Yang, Luxi; Xu, Shuaimei; Stedtfeld, Robert D.; Chen, Qiongyun; Liu, Jingjing; Zhang, Xiang; Xu, Hui; Ren, Jianlin

doi:10.3389/fmicb.2020.00383

Cited by 62 publications

(89 citation statements)

References 62 publications

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“…The inclusion criteria were as follows: (1) no allergic history and no family history of allergic diseases; (2) sausage or snake shaped stool; and (3) voluntary participation in this study. The exclusion criteria of non-AR populations were based on previously reported [ 24 ] and individuals with allergy disease, such as AR, asthma, eczema and urticarial were further excluded.…”

Section: Methodsmentioning

confidence: 99%

“…The procedure for DNA extraction of fecal samples (approximately 0.25 g) from non-AR healthy individuals and AR patients, the purity and concentration of the isolated DNAs, the 16S rRNA V3-V4 hypervariable region amplification for the library construction and paired-end sequencing were based on previous procedure [24]. The bioinformatics analysis procedure for raw reads were according to the previously described [24,29].…”

Section: Lab Work and The Bioinformatics Analysismentioning

confidence: 99%

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Gut microbial characteristics of adult patients with allergy rhinitis

Zhu

Wan

et al. 2020

Microb Cell Fact

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Background Although recent studies have indicated that intestinal microbiota dweller are involved in the pathogenesis of allergy rhinitis (AR), the influence of gut microbiota on AR adult has not been fully elucidated yet. Hence, we carried out this study to uncover the distinctive bacterial taxa that differentiate allergy rhinitis patients from healthy individuals. Feces samples from thirty three AR patients and thirty one healthy individuals were analyzed by 16S rRNA gene sequencing. Results Results showed that the bacterial diversity in AR group was significantly higher than that of the non-AR group. Bacterial communities between AR and non-AR group were significantly differentiated as revealed by Principal coordinates analysis (PCoA) and the variation within non-AR were higher than that of the counterpart. Firmicutes, Fusobacteria, Actinobacteria, Cyanobacteria and Chloroflexi were the significantly differed phyla taxa and the top significantly distinguished bacterial genus included Prevotella_9, Phascolarctobacterium, Roseburia, Megamonas, Alistipes, Lachnoclostridium and Fusobacterium. The higher network complexity in AR group were dominated by taxa belonging to Firmicutes. The predicted function, alpha linolenic acid metabolism and bacterial invasion of epithelial cells pathway were higher in non-AR group while gonadotropin-releasing hormone (GnRH) signaling pathway, Fc γ-R mediated phagocytosis and endocytosis were higher in AR patients. Although the bacterial diversity between moderate and severe AR patients showed no significant difference, the significant correlation between featured genus and total nasal symptom score or rhinoconjunctivitis quality of life questionnaire, such as Butyricicoccus and Eisenbergiella, revealed the potential to intervene the AR status by means of gut microbiota. Conclusions In conclusion, patients with allergy rhinitis had distinguished gut microbiota characteritics in comparison with healthy controls. The results suggest that gut microbiota might play crucial roles in influencing the course and different symptoms of AR. Trial registration ChiCTR, ChiCTR1900028613. Registered 29 December 2019, https://www.chictr.org.cn/showproj.aspx?proj=47650.

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Section: Methodsmentioning

confidence: 99%

Section: Lab Work and The Bioinformatics Analysismentioning

confidence: 99%

Gut microbial characteristics of adult patients with allergy rhinitis

Zhu

Wan

et al. 2020

Microb Cell Fact

Self Cite

View full text Add to dashboard Cite

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“…If keystone species can be considered as "indicator taxa", another term, which have been defined as those taxa that are highly indicative of a particular experimental treatment or environmental condition [87] is still unclear. The concept of indicator taxa has gained much interest from the practical point of view and was transferred from macroecology into microbiome research, and is now frequently used, e.g., in studies assessing the impact of agricultural practices on microbiomes [88] or of diseases on the human microbiota [89]; as here, simple and highly standardized qPCR-based approaches can be used for analysis.…”

Section: Microbial Network and Interactionsmentioning

confidence: 99%

Microbiome definition re-visited: old concepts and new challenges

et al. 2020

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The field of microbiome research has evolved rapidly over the past few decades and has become a topic of great scientific and public interest. As a result of this rapid growth in interest covering different fields, we are lacking a clear commonly agreed definition of the term "microbiome." Moreover, a consensus on best practices in microbiome research is missing. Recently, a panel of international experts discussed the current gaps in the frame of the European-funded MicrobiomeSupport project. The meeting brought together about 40 leaders from diverse microbiome areas, while more than a hundred experts from all over the world took part in an online survey accompanying the workshop. This article excerpts the outcomes of the workshop and the corresponding online survey embedded in a short historical introduction and future outlook. We propose a definition of microbiome based on the compact, clear, and comprehensive description of the term provided by Whipps et al. in 1988, amended with a set of novel recommendations considering the latest technological developments and research findings. We clearly separate the terms microbiome and microbiota and provide a comprehensive discussion considering the composition of microbiota, the heterogeneity and dynamics of microbiomes in time and space, the stability and resilience of microbial networks, the definition of core microbiomes, and functionally relevant keystone species as well as co-evolutionary principles of microbe-host and inter-species interactions within the microbiome. These broad definitions together with the suggested unifying concepts will help to improve standardization of microbiome studies in the future, and could be the starting point for an integrated assessment of data resulting in a more rapid transfer of knowledge from basic science into practice. Furthermore, microbiome standards are important for solving new challenges associated with anthropogenic-driven changes in the field of planetary health, for which the understanding of microbiomes might play a key role.

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“…Gamma- Proteobacteria was enriched in new-born mice and depleted in normal adult microbiota, which was regulated by a gamma- Proteobacteria -specific IgA response, thus higher abundance of gamma- Proteobacteria was associated with sustained intestinal inflammation [ 46 ] which may also perpetrate kidney injury. Phylum Fusobacteria and genus Fusobacterium were expanded in patients with CKD, and were also enriched in patients with colorectal cancer [ 47 ] and chronic hepatitis B disease [ 48 ]. The potential ability of Fusobacterium for inhibiting T-cell proliferation and inducing T-cell apoptosis may perpetrate local or system immune disorder [ 47 ] leading to immune-mediated kidney diseases.…”

Section: Discussionmentioning

confidence: 99%