I would like to thank my supervisor Prof. M. Zervakis for his support and motivation in carrying out this project. I would also like to thank the members of the supervising committee, Prof. Minos Garofalakis, Prof. Dionissios Hristopulos, Assoc. Prof. Alexandros Potamianos, Assoc. Prof. Konstantinos Balas, Assoc. Prof. Evripidis Petrakis, Assoc. Prof. Georgios Karystinos for their time and useful remarks. Many thanks also due to Prof. Sabine Van Huffel, Prof. Dirk Timmerman and Ben Van Calster and the ESAT research team at Catholic University of Leuven, Belgium, for their help and guidance in significant parts of this research. Financial support for this study was provided in part by EU: BIOPATTERN (FP6-2002-IST 508803). The funding agreement ensured the authors' independence in designing the study, interpreting the data, writing, and publishing the report.